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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (18): 25-32.doi: 10.11924/j.issn.1000-6850.casb2025-0548

• 农学·农业基础科学 • 上一篇    下一篇

西藏藜麦种子可培养内生细菌多样性

王生萍1(), 卓玛曲措2, 降志兵1, 谈建鑫1, 同坚1()   

  1. 1 西藏自治区农牧科学院农业研究所/省部共建青稞和牦牛种质资源与遗传改良国家重点实验室， 拉萨 850032
    2 西藏农牧大学植物科学学院， 西藏林芝 860000
  • 收稿日期:2025-07-01 修回日期:2025-12-11 出版日期:2026-09-25 发布日期:2026-09-24
  • 通讯作者:
    同坚，男，1985年出生，陕西渭南人，高级农艺师，本科，学士，研究方向：粮油作物轻简高效栽培技术的集成与示范推广，主持省部级以上科研项目6项，发表论文8篇，内容涉及青稞肥效调控、轻简栽培技术规范等，并参与制订地方标准10项。通信地址：850032 西藏自治区拉萨市城关区金珠西路130号 西藏自治区农牧科学院农业研究所，E-mail：。
  • 作者简介:

    王生萍，女，1995年出生，研究实习员，硕士研究生，硕士，主要从事高原作物高产栽培研究。通信地址：850032 西藏自治区拉萨市城关区金珠西路130号 西藏自治区农牧科学院农业研究所，E-mail：。

  • 基金资助:
    地市级科技计划项目“阿里高寒农区青稞新品种高产高效栽培技术研究与应用”(QYXTZX-AL2024-02)

Diversity of Culturable Endophytic Bacteria from Seeds of Chenopodium quinoa in Xizang

WANG Shengping1(), ZHUO Maqucuo2, JIANG Zhibing1, TAN Jianxin1, TONG Jian1()   

  1. 1 Institute of Agriculture, Xizang Autonomous Region Academy of Agricultural and Animal Husbandry Sciences/ State Key Laboratory of Barley and Yak Germplasm Resources and Genetic Improvement, Lhasa 850032
    2 College of Plant Science, Xizang Agriculture and Animal Husbandry University, Nyingchi, Xizang 860000
  • Received:2025-07-01 Revised:2025-12-11 Published:2026-09-25 Online:2026-09-24

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摘要：

为了探究西藏藜麦种子内生可培养细菌的种类及其多样性，丰富西藏地区藜麦种子内生菌资源。采集西藏拉萨、日喀则、林芝三地藜麦种子，通过传统的分离培养法，对分离得到的内生细菌进行种类鉴定及群落结构分析。实验共成功分离并鉴定出1675株内生细菌，结合16S rRNA基因序列分析与形态特征，将其归类为3门4纲7目16科35属118种，其中优势种群为Pantoea agglomerans、Bacillus altitudinis及Bacillus siamensis。实验共采用6种培养基，其中NA培养基的分离频率最高，达23.1%（387株）。α-多样性分析结果显示，不同地区的藜麦种子内生可培养细菌群落结构存在差异：拉萨地区的细菌物种优势度与均匀度较高，林芝地区物种丰富度及相对丰度优于其他两地。总体而言，西藏藜麦种子中蕴藏着种类丰富、多样性高的可培养内生细菌，具有重要的研究价值和潜在的应用前景。

关键词: 西藏藜麦, 内生可培养细菌, 优势菌群, 16S rRNA鉴定, α-多样性, 分离培养基, 物种丰富度, 地区差异性

Abstract:

To explore the species and diversity of culturable endophytic bacteria of quinoa seeds in Xizang, and to enrich the resources of endophytic bacteria in quinoa seeds, quinoa seeds were collected from Lhasa, Shigatse, and Nyingchi in Xizang. The species and community structure characteristics of their endophytic bacteria were identified using traditional isolation and cultivation methods. A total of 1,675 strains of endophytic bacteria were obtained through isolation and cultivation. Based on 16S rRNA sequence analysis and morphological characteristics, they were classified into 118 species, 35 genera, 16 families, 7 orders, 4 classes, and 3 phyla. The dominant species were Pantoea agglomerans, Bacillus altitudinis, and Bacillus siamensis. Six types of culture media were used in the experiment, with NA medium showing the highest isolation frequency of 23.1% (387 strains). α-diversity analysis revealed that the culturable endophytic bacteria of quinoa seeds in Lhasa exhibited higher species dominance and evenness, while Nyingchi showed better species richness and relative abundance compared to other two regions. The culturable endophytic bacteria in Xizang quinoa seeds are rich and diverse, demonstrating potential research value.

Key words: Xizang Chenopodium quinoa, culturable endophytic bacteria, dominant bacterial community, 16S rRNA gene identification, α-diversity, isolation media, species richness, regional differences

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