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中国农学通报 ›› 2009, Vol. 25 ›› Issue (5): 18-21.

所属专题: 生物技术 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

崂山奶山羊瘤胃微生物总DNA的提取方法优化

王利华,隋昶生   

  • 收稿日期:2008-12-03 修回日期:2009-02-04 出版日期:2009-03-05 发布日期:2009-03-05

Extraction method optimizing of total DNA in rumen microorganism of Laoshan dariy

  • Received:2008-12-03 Revised:2009-02-04 Online:2009-03-05 Published:2009-03-05

摘要: 本试验对对瘤胃微生物总DNA提取方法进行了改进,将冻融法和CATB法相结合,可以将细菌、真菌和古细菌在内的各种微生物的细胞壁充分的粉碎,且DNA提取效果稳定,可以获得足够大的DNA片段(23kb),可以满足后续试验的需要

关键词: “三农”问题, “三农”问题, 西部地区, 农业产业化

Abstract: The present experiment was conducted to assess DNA extraction method of freezing-thawing and methods combined freezing-thawing and CATB. Results showed that the methods combination of freezing-thawing and CATB could smash cell wall of varieties of micro-organisms including bacteria and Fungus. DNA extraction was stable by this technique and DNA fragments were large enough to be applied in follow-up research. It could be concluded that freezing-thawing and CATB methods combination could extract the total rumen microbial DNA.