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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (5): 14-17.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

鸡传染性支气管炎病毒RT-PCR检测方法的建立

程晓霞,王劭,朱小丽,陈仕龙,林锋强,陈少莺   

  • 收稿日期:2008-11-27 修回日期:2009-01-12 出版日期:2009-03-05 发布日期:2009-03-05

Development of RT-PCR method for detecting Infectious Bronchitis Virus

Cheng Xiaoxia,, Wang Shao,, Zhu Xiaoli, Cheng Shilong,, Lin Fengqiang,, Chen Shaoying,   

  • Received:2008-11-27 Revised:2009-01-12 Online:2009-03-05 Published:2009-03-05

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摘要： 根据GenBank上发表的鸡传染性支气管炎病毒（IBV）基因序列，针对IBV M基因全序列设计合成了一对引物, 以IBV疫苗株为模板, 建立了检测IBV的RT-PCR方法。应用该方法对IBV疫苗株RNA进行扩增, 获得与预期大小相符, 长度为1105bp的特异性目的片段；敏感性测定该RT-PCR可扩增到18pg的IBV-M-cDNA。结果表明建立的RT-PCR方法对IBV的检测敏感性高、特异性强，可用于IBV感染的诊断和流行病学调查。

关键词: 三峡库区, 三峡库区, 消落区, 对策

Abstract: A pair of primers were designed and synthesized based on the sequences of the IBV genomes, and the RT-PCR method to detect the infectious Bronchitis virus （IBV）were developed.The 1105 bp specifical fragment were obtained from the IBV vaccine strain RNA by this RT-PCR.The sensitivity of RT-PCR reached to 2.85 ng IBV-M-cDNA.These results showed that the RT-PCR method to detect IBV were of better specificity and higher sensibility. The results showed that this PCR technique was specific and applied to IBV diagnoses and epidemiology investigation．