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中国农学通报 ›› 2011, Vol. 27 ›› Issue (7): 1-5.

所属专题: 生物技术 玉米

• 农学 农业基础科学 •    下一篇

转Bt毒蛋白基因玉米的研究进展

谢树章 雷开荣 林清   

  • 收稿日期:2010-10-09 修回日期:2010-11-02 出版日期:2011-04-05 发布日期:2011-04-05
  • 基金资助:

    国家转基因生物新品种培育重大专项“抗逆转基因玉米新品种培育”课题;重庆市科技创新能力建设专项

Research Progress of Maize Transformation with Bt Toxin Protein Gene

Kai-rong Lei Qing LIn   

  • Received:2010-10-09 Revised:2010-11-02 Online:2011-04-05 Published:2011-04-05

摘要:

此文首先回顾了国内外抗虫转基因玉米研究发展历程,归纳总结了转Bt毒蛋白基因玉米的常用转化方法和转基因玉米转化体的鉴定方法,对转Bt毒蛋白基因玉米后续实验的抗虫性分析鉴定及遗传稳定性评价与利用也进行了介绍。进而探讨了Bt毒蛋白基因在玉米遗传转化上善待解决的问题以及未来的发展方向。认为应加大力度对瓶颈技术进行深入研究,以期转Bt毒蛋白基因玉米能够获得更好的发展。

关键词: 酉阳, 酉阳

Abstract:

In this paper, firstly, we review research progress of transgenic maize with insect resistant, classify and summarize main approaches of maize transformation with Bt toxin protein gene and screening of transgenic lines, then the bioassay for insect resistance and evaluation and utilization of genetic stability are introduced, too. Finally, we discuss the technical difficulties and prospect of transgenic maize with insect resistant. We hold that in order to promote better development of maize transformation with Bt toxin protein gene, the key technologies must be further study positively.

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