欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (27): 225-229.

所属专题： 玉米

• 植物保护 农药 • 上一篇    下一篇

云南玉米大斑病群体遗传多样性的RAPD分析

姜开梅 范静华 刘群   

  • 收稿日期:2012-01-05 修回日期:2012-03-21 出版日期:2012-09-25 发布日期:2012-09-25
  • 基金资助:

    云南省科技攻关项目

RAPD Analysis Among Isolates of Exserohilum turcicum in Maize from Yunnan Province

  • Received:2012-01-05 Revised:2012-03-21 Online:2012-09-25 Published:2012-09-25

PDF (PC)

756

摘要：

为了从分子水平上了解云南玉米大斑病菌遗传变异情况，为玉米大斑病菌种群致病力分化和病害有效控制提供理论依据。本研究采用RAPD分析技术，对采自云南省部分地区的56个大斑病菌株进行了RAPD分析。对供试菌株间的遗传距离进行聚类分析并构建系统树状图，聚类分析结果表明，供试56个菌株被划分为10个遗传聚类组，RAPD分组与菌株的地理来源无明显相关性。云南省不同地区的玉米大斑病菌株整体亲缘关系相近，但各菌株间存在遗传差异。

关键词: 土壤健康, 土壤健康

Abstract:

In order to study the genetic variation of Yunnan Exserohilum turcicum from the molecular level, and provide theoretical basis for Exserohilum turcicum pathogenicity virulence differentiation and effective control of disease, Using random amplified polymorphic DNA technique, fifty-six Exserohilum turcicum isolates on maize that from some areas of Yunnan Province were tested for RAPD. Based on the distance, a dendrogram was constructed of the 56 strains tested, 10 genetic groups were formed. There was no correlation found between RAPD group and the geographic origin. The analysis showed that some strains from one area were closely related to some from another area. Nonetheless, rich genetic variation existed among the tested strains.