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中国农学通报

中国农学通报 ›› 2014, Vol. 30 ›› Issue (28): 164-168.doi: 10.11924/j.issn.1000-6850.2014-1267

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高良姜种子超低温保存研究

曾琳,何明军,陈葵,郑希龙   

  1. 中国医学科学院药用植物研究所海南分所,中国医学科学院药用植物研究所海南分所,中国医学科学院北京协和医学院,中国医学科学院北京协和医学院
  • 收稿日期:2014-04-30 修回日期:2014-04-30 接受日期:2014-09-05 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 曾琳
  • 基金资助:
    国家科技重大专项“中药材种子种苗和种植（养殖）标准平台”(2012ZX09304006)；中央级公益性科研院所基本科研业务费项目“白木香、 降香等顽拗型药用植物种质超低温保存技术研究”(2012HNB02)；国家基本药物所需中药原料资源调查和监测项目“国家基本药物所需中药材 种苗繁育基地和种质资源库建设”[国中医药科技中药便函(2012)157、158 号]

Study on Cryopreservation of Alpinia officinarum Hance Seeds

  • Received:2014-04-30 Revised:2014-04-30 Accepted:2014-09-05 Online:2014-10-15 Published:2014-10-15

摘要： 为解决高良姜种子长期保存的问题，以高良姜的成熟种子为材料，研究含水量和玻璃化处理对其活力的影响，以及快速冷冻法和玻璃化冷冻法对种子超低温保存的影响。结果表明：（1）种子含水量在10.77%~27.25%时，其活力仍保持在60%以上，当含水量由10.77%降至7.86%时，其活力降至40%以下；（2）玻璃化处理后的种子活力低于未玻璃化处理的种子活力；（3）经液氮超低温保存后，含水量为21.10%~27.25%的种子活力降至50%以下甚至为0%；含水量为10.77%~15.61%的种子活力仍保持在60%以上；其中含水量13.58%的种子活力最高，并且玻璃化冷冻处理的种子活力(72.67%)高于快速冷冻处理的种子活力(65.81%)。据此认为，液氮超低温长期保存高良姜种子是可行的。

关键词: 生态适应性, 生态适应性

Abstract: In order to solve the problem of long-term preservation of Alpinia officinarum Hance seeds, the mature seeds of Alpinia officinarum Hance were used as materials, to study the effect of moisture content and vitrification on their vigor, as well as the effect of rapid freezing and vitrification freezing method for seeds cryopreservation. The results showed that: (1) when the seed moisture content was in the range of 10.77%-27.25%, the viability still remained at more than 60%, however, when the seed moisture content decreased from 10.77% to 7.86%, the viability dropped to below 40%; (2) seed vigor after vitrification treatment was lower than that without vitrification treatment; (3) after cryopreservation in liquid nitrogen, the seed vigor dropped to below 50% or even 0% when the seed moisture content was in the range of 21.10%-27.25%; the seed vigor remained at more than 60% when the seed moisture content was in the range of 10.77%-15.61%; furthermore, the seed vigor was the highest when the moisture content was 13.58%; finally, the seed vigor of vitrification freezing (72.67%) was higher than that of rapid freezing (65.81%). The results demonstrated the feasibility of cryopreservation for long-term storage of seeds of Alpinia officinarum Hance.