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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (18): 135-141.doi: 10.11924/j.issn.1000-6850.casb19030138

所属专题： 马铃薯

• 植物保护 农药 • 上一篇    下一篇

甘薯茎基部腐烂病调查及病原鉴定

黄立飞1, 刘伟明2, 刘也楠3, 何贤彪3   

  1. 1.广东省农业科学院作物研究所;2.台州科技职业学院;3.台州市农业科技研究院
  • 收稿日期:2019-03-30 修回日期:2019-06-11 接受日期:2019-05-22 出版日期:2019-07-01 发布日期:2019-07-01
  • 通讯作者: 刘伟明
  • 基金资助:
    国家现代农业（甘薯）产业技术体系“鲜食型甘薯改良岗”(CARS-10-C-17)，“广州综合试验站”(CARS-10-B-4)；2017 年广东省级农业 发展与农村工作专项“优质抗逆加工专用甘薯新品种创制及应用”；浙江省农业（粮食）新品种选育重大科技专项“甘薯、山药种质筛选及新品种 选育”(2016C02050-7-3)；台州市农业类重点科技项目“甘薯根茎部腐烂病害的病原菌鉴定、发生危害规律及防控技术研究”(1701ny12)。

Investigation on the stem base rot of sweetpotato and identification of pathogen species

  • Received:2019-03-30 Revised:2019-06-11 Accepted:2019-05-22 Online:2019-07-01 Published:2019-07-01

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摘要： 2012年以来浙江省台州市及其周边市县甘薯产区发生了大面积的甘薯茎基部腐烂病,导致当地甘薯种植面积大幅度下滑。为明确浙江省甘薯茎基部腐烂病的发生情况、病原组成及种类,本团队于2015年至2018年对浙江省11个县市区进行了田间调查并采集127份病样分离鉴定病原菌,结果表明浙江省甘薯茎基部腐烂病主要存在4种主要症状,根据病菌的致病性、形态特征、ITS和16S rDNA序列分析鉴定,主要鉴定到达旦提狄克氏菌(Dickeya dadantii)、齐整小核菌(Sclerotium rolfsii)、拟轮枝镰孢菌(Fusarium verticillioides )、腐皮镰刀菌(F. solani)、尖镰刀菌(F. oxysporum)、甘薯间座壳菌(Diaporthe batatas)、毁坏性拟茎点霉(Phomopsis destruens)和爪哇镰孢(F. javanicumS)等8种致病病菌,各地区病原菌组成分布均有所不同,采集的样品普遍为多种致病菌复合侵染危害。

关键词: 新型肥料、释放特征、甘薯产量、氮效率, 新型肥料、释放特征、甘薯产量、氮效率

Abstract: Sweetpotato stem base rot has occurred in the sweetpotato production area in the Taizhou city and its surrounding cities in Zhejiang Province since 2012, caused a significant decline in the local sweetpotato planting area. To identify the occurrence of sweetpotato stem base rot, and pathogenic composition, the field investigation carried out in 11 counties in Zhejiang province from 2015 to 2018, 127 samples were collected for isolation and identification of pathogens. The results showed that there were four main symptoms in the basal rot of sweetpotato in Zhejiang Province. Based on pathogenicity test, morphological characteristics, and sequence analysis of internal transcribed spacer (ITS) region and partial 16S rDNA, 8 pathogens including Dickeya dadantii, Sclerotium rolfsii, Fusarium verticillioides, F. solani, F. oxysporum, Diaporthe batatas, Phomopsis destruens and F. javanicum were identified. The distribution and composition of pathogen in different regions is different, compound infection of multiple pathogens were found to be more common in collected samples of root rot symptom.