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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (10): 179-182.

所属专题： 园艺

• 林学 园艺 园林 • 上一篇    下一篇

根癌农杆菌介导番茄‘白果强丰’遗传转化体系优化

李立芹   

  • 收稿日期:2010-11-08 修回日期:2010-12-28 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    植物生理学与生物化学国家重点实验室开放课题

Study on Transformation Optimization by Agrobacterium tumefaciens Mediated in Tomato

  • Received:2010-11-08 Revised:2010-12-28 Online:2011-05-15 Published:2011-05-15

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摘要：

以番茄无菌苗的子叶为外植体，通过农杆菌介导法对其遗传转化体系进行了优化，结果表明：外植体在MS+2 mg/L 6-BA+0.2 mg/L IAA的进行2天的预培养后，用农杆菌EHA105（浸染浓度为OD600=0.6）浸染6 min，转化效率最高，经过PCR检测初步证明NPTII基因已整合到番茄再生植株中，本研究建立了高效番茄‘白果强丰’子叶农杆菌介导的遗传转化体系。

关键词: 生物学特性, 生物学特性

Abstract:

Tomato cultivars were used as the materials, and cotyledon was cultured to optimize the transformation system via Agrobacterium tumefaciens. The results showed that the highest transformation efficiency was obtained when the explants were preconditioned on preconditioning medium for 2 days prior to infect with Agrobacterium tumefaciens (OD600=0.6) for 6 min growing on the culture medium of MS+2.0 mg/L 6-BA+0.2 mg/L IAA. It was proved that the target NPTII gene had been integrated into the genome of regenerated plants by PCR analysis. This study established a high efficient genetic transformation system of tomato cytoledons by Agrobacterium tumefaciens mediated.