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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (10): 173-178.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

转il-4基因番茄及其杂交F1代的筛选与表现型分析

彭洪雨 张鹏 及晓宇 于丽杰   

  • 收稿日期:2010-11-25 修回日期:2011-01-16 出版日期:2011-05-15 发布日期:2011-05-15
  • 基金资助:

    黑龙江省普通高等学校骨干教师创新能力资助计划;黑龙江省自然科学基金

Selection and Phenotypic Analysis of Transgenic Tomato with Interleukin-4 Gene and its F1 Hybrids

  • Received:2010-11-25 Revised:2011-01-16 Online:2011-05-15 Published:2011-05-15

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摘要：

为完善转il-4基因番茄的筛选及鉴定工作，本实验比较了il-4基因在PPT抗性筛选与非PPT抗性筛选植株中的阳性率，对il-4基因在T6代及杂交F1代番茄中的传递规律和植株表现型进行调查。本文对L402、‘中疏4号’、‘大黄’三个品种的T6代及杂交F1代植株进行PPT抗性筛选及非PPT抗性筛选，特异性的PCR扩增、PCR-Southern杂交。研究结果表明，il-4基因在非PPT抗性筛选植株中的阳性率高于PPT筛选植株，T6代及F1代植株中il-4基因的分离情况不符合孟德尔分离规律，其分离情况远远低于3:1的分离比率。转il-4基因番茄与非转基因植株（对照）相比发现，转基因植株在一至多个性状上发生了不同程度的变异，杂交F1代与亲本T6代植株相比，杂交F1代有少数组合存在杂种优势。

关键词: 影响因素, 影响因素

Abstract:

In order to improve the selection and detection of transgenic tomato with interleukin-4 (il-4) gene, in the present paper, positive rate of il-4 gene in the individuals after non-PPT resistance selection was compared with those after PPT resistance selection, then the hereditary patterns and phenotype of il-4 gene of T6 transgenic individuals and F1 hybrids was analyzed. Then, DNA samples were extracted from the leaves of T6 transgenic plants and F1 hybrids of L402, ‘Zhongshu4’ and ‘Dahuang’ varieties, performed PPT resistance selection and non-PPT resistance selection, PCR amplification and PCR-Southern detection. The results showed that, the screening rate of target gene after non-PPT resistance selection was higher than that after PPT resistance selection. The segregation ratios of il-4 gene of T6 and F1 individuals were much lower than 3:1, and it didn’t conform to Mendelian inheritance. Compared to CK, variations are discovered in more than 1 character of il-4 transgenic tomato plants. A few F1 hybrids have heterosis compared to T6 transgenic individuals.