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中国农学通报 ›› 2011, Vol. 27 ›› Issue (13): 135-138.

• 林学 园艺 园林 • 上一篇    下一篇

芦荟蒽醌类物质的提取及其抑菌性研究

秦伟 乔丹 宝力德   

  • 收稿日期:2010-11-30 修回日期:2011-01-27 出版日期:2011-06-05 发布日期:2011-06-05
  • 基金资助:

    内蒙古农业大学项目

Extraction of Aloe Anthraquinones and Analysis of Its Antibacterial Effect

  • Received:2010-11-30 Revised:2011-01-27 Online:2011-06-05 Published:2011-06-05

摘要:

本试验利用超声波提取库拉索芦荟中蒽醌类物质,测定其含量,并对枯草芽孢杆菌、大肠杆菌及金黄色葡萄球菌进行抑菌性研究。用滤纸片法、固体稀释法及最小抑菌浓度法(MIC)分别检测芦荟蒽醌类物质对各种菌的抑菌性,并比较分析3种方法的抑菌效果。由固体稀释法测定芦荟蒽醌提取液的抑菌效果可知,蒽醌类物质百分浓度越高,菌落数越少,显示出越高的抑菌性。最小抑菌浓度法(MIC)测出,芦荟蒽醌提取液对大肠杆菌、金色葡萄球菌的MIC为90%,对枯草芽孢杆菌的MIC为80%,显示出MIC都较高,说明低浓度的芦荟蒽醌类抑菌能力有限,需要较高的浓度才能抑制菌生长。

关键词: 植物表达载体, 植物表达载体

Abstract:

In this study, the Aloe vera L. anthraquinones has been extracted by ultrasonic extraction and its content has been determined. The antibacterial effect of the Aloe anthraquinones on the Bacillus subtilis, Escherichia coli, Staphylococcus aureus has been investigated. The filter paper method, solid dilution method and minimum inhibitory concentration (MIC) method have been used to determine the antibacterial effect of the Aloe anthraquinones, and the efeects of this three methods have been compared. The solid dilution method showed that the high concentration of Aloe anthraquinone extract had the significant antibacterial effect. The minimum inhibitory concentration (MIC) was 90% for E. coli and S. aureus, and 80% for B. subtilis. The MIC of the Aloe anthraquinones showed a higher values, that meant antibacterial activity of Aloe anthraquinones was limited, only the Aloe anthraquinones with higher concentrations can inhibit the bacteria growth.