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中国农学通报 ›› 2011, Vol. 27 ›› Issue (13): 139-143.

• 林学 园艺 园林 • 上一篇    下一篇

蝴蝶兰原球茎的增殖研究与解剖学观察

马生健 陈玉青 廖婷 郭志 曾富华   

  • 收稿日期:2011-01-19 修回日期:2011-03-10 出版日期:2011-06-05 发布日期:2011-06-05
  • 基金资助:

    2005EA780070

The Effect of Organic Compounds on the Propagation of Phalaenosis PLB and Anatomical Observation

  • Received:2011-01-19 Revised:2011-03-10 Online:2011-06-05 Published:2011-06-05

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摘要：

蝴蝶兰是名贵花卉，市场需求量大，工厂生产大多通过组织培养无性繁殖来达到扩增的目的。本研究以蝴蝶兰（Phalaenopsis）的原球茎（Protocorm-like body，以下简称PLB）为外植体在VW + 3.0 mg/L 6-BA+ 0.2 mg/L NAA+ 20 g/L 蔗糖+ 16 g/L琼脂+ 1 g/L活性炭中分别添加不同浓度（10%、15%、20%）的有机物（椰乳、椰汁、马铃薯汁、香蕉泥、水解酪蛋白）进行培养，比较不同浓度的有机添加物对蝴蝶兰PLB增殖的影响。结果表明：5种有机添加物中，对蝴蝶兰PLB增殖效果总体影响由好至差依次为椰乳、椰汁、马铃薯汁、香蕉泥、水解酪蛋白。椰乳的最佳增殖浓度是15%，椰汁的是20%，马铃薯汁的是10%，香蕉泥的是15%，而水解酪蛋白的10%、15%、20%这3个浓度都偏高，抑制了PLB的增殖。对蝴蝶兰初生、次生各阶段PLB和由PLB所诱导分化的叶芽进行了切片观察，结果表明初生PLB的周边细胞排列紧密，中间细胞则稀疏，没有规则；次生PLB的细胞则大小比较均一，次生PLB发生于初生PLB的下半部，叶芽则发生在球茎的上半部区域，在初生PLB中还存在肾型的气孔和未知功能的棒状结构。

关键词: 植物病原菌, 植物病原菌

Abstract:

The Phalaenopsis is a kind of expensive flower and its needt is large in the marke, the tissue culture of the Phalaenopsis can meet its great need of the market. The Phalaenopsis PLB as explants were inoculated on the improved VW + 3.0 mg/L 6-BA + 0.2 mg/L NAA + 20 g/L surose + 16 g/L agar medium + 1 g/L active carbon adding five different organic compounds (coconut milk, coconut juice, banana mud, potato, casein hydrollysate) with different concentrations (10%,15%,20%). The results showed that five organic compounds which had effect on PLB propagation from high to low were coconut milk, coconut juice, potato, banana mud and casein hydrollysate, respectively. The best concentration of coconut milk for propagation was 15%, coconut juice was 20%, potato juice was 10%, banana mud was 15%, but 10%, 15%, 20% were so high to casein hydrolyzed, so restrained the propagation of Phalaenosis PLB. Primary protocorm-like body (PLB), secondary PLB and leaf bud induced by PLB of Phalaenopsis were observed by free-hand section method, and their connection and differentiation were analyzed. The results showed that the inner cells in primary PLB were big and irregular and the cells in secondary PLB were compacted and homogeneous. Secondary PLB were formed from the hyposphere of primary PLB, and leaf bud was formed from the episphere of primary PLB. Semi-moon-shaped stoma and “multi-rod structure” were found in primary PLB, and “multi-rod structure” function was unknown.