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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (12): 52-55.

所属专题： 生物技术； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

中国小麦LB0288中抗叶锈病基因的鉴定

齐爱勇 李星 赵振杰   

  • 收稿日期:2010-12-28 修回日期:2011-02-21 出版日期:2011-05-25 发布日期:2011-05-25
  • 基金资助:

    小麦抗叶锈病相关基因的克隆;小麦抗叶锈病基因Lr41抗病相关基因的克隆及分析

Identification of Leaf Rust Resistance Gene in Wheat Line LB0288

  • Received:2010-12-28 Revised:2011-02-21 Online:2011-05-25 Published:2011-05-25

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摘要：

明确中国小麦LB0288中所含的抗叶锈病基因，找到与其紧密连锁的DNA分子标记。将小麦LB0288和感病小麦品种Thatcher杂交，获得F1、F2代群体，用叶锈菌小FHTT分别对双亲及其杂交后代进行叶锈鉴定并进行标记分析。抗性鉴定结果表明F2代群体时呈现一对显性基因的抗感分离比例，经过亲本和抗感池间标记筛选以及F2代群体的标记检测，位于5DL的SSR标记barc144与抗病基因连锁，遗传距离为5.3 cM，同时Lr1的STS标记与之共分离，根据该基因的抗性特点和染色体位置推断为Lr1。此实验通过抗性鉴定、遗传分析和分子标记等手段确定LB0288中含有小麦抗叶锈病基因Lr1。

关键词: 籼粳血缘, 籼粳血缘

Abstract:

The objective of this study was to identify leaf rust resistance gene and its closely linked molecular markers in LB0288. In order to make use of the leaf rust resistance gene in LB0288 effectively, F1 and F2 populations from the cross LB0288/Thatcher were tested with pathotype FHTT for following marker analysis. The results indicated that LB0288 carried a single dominant resistance gene Lr1, closely linked to the STS markers of Lr1 and SSR marker barc144, with genetic distances of 0 cM and 5.3 cM, respectively. Based on the chromosomal location and seedling reactions, it could be concluded that LB0288 carried resistance gene Lr1.

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