欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (12): 48-51.

所属专题： 生物技术； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

3个受白粉菌诱导的小麦抗病相关基因的表达分析

王俊美 徐红明 刘红彦 李敏 康振生   

  • 收稿日期:2010-12-06 修回日期:2011-01-04 出版日期:2011-05-25 发布日期:2011-05-25
  • 基金资助:

    河南省杰出人才基金

Expression Analysis of Three Wheat Resistance-related Genes Induced by Blumeria Graminis

  • Received:2010-12-06 Revised:2011-01-04 Online:2011-05-25 Published:2011-05-25

PDF (PC)

1404

摘要：

小麦白粉病是小麦生产上的重要病害。研究小麦抗白粉病的分子机理，利用抗病基因是控制小麦白粉病的有效途径。此研究根据前期白粉菌诱导后获得的可能与农家小麦品种红蚰麦抗病相关序列的分析结果，合成了几丁质酶，交替氧化酶（Alternative oxidase，AOX）以及MAP激酶（促分裂素原活化蛋白激酶mitogen-activated protein kinases）类似物3个基因的定量PCR引物，实时分析了在白粉菌诱导条件下‘红蚰麦×豫麦13’的F3代纯合抗病植株与感病植株两种互作组合中3个基因的时空表达模式，该研究为揭示‘红蚰麦’抗白粉菌的分子机理提供了依据。

关键词: 松褐天牛, 松褐天牛, 空间格局, 聚集分布, 环境因子

Abstract:

Wheat Powdery mildew is one important disease in wheat yield. It is an effective method for the control of the disease to study molecular mechanism of wheat resistance to powdery mildew and utilize resistance genes. Based on obtained resistance-related sequences from wheat landrace ‘Hongyoumai’ induced by Blumeria graminis, three pairs of primers were synthesized to analyze expression patterns of three genes chitinase, alternative oxidase and MAP kinase homolog in homogeneous resistant F3 progeny plants and susceptible F3 progeny plants derived from ‘Hongyoumai×Yumai13’, which respectively, represent different interaction patterns between plant and pathogen , results will help elucidate molecular mechanism of ‘Hongyoumai’ resistance to powdery mildew.

中图分类号: