欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (19): 121-124.

• 林学 园艺 园林 • 上一篇    下一篇

朝鲜百合鳞茎诱导及再生体系建立

陈丽静 葛菱 张丽 郭志富 孙红梅 李天来 陶承光   

  • 收稿日期:2011-01-30 修回日期:2011-03-19 出版日期:2011-08-05 发布日期:2011-08-05
  • 基金资助:

    辽宁省教育厅项目;中国博士后科学基金面上项目资助

Induction of Lilium amabile Bulb and Establishment of Regeneration System

  • Received:2011-01-30 Revised:2011-03-19 Online:2011-08-05 Published:2011-08-05

PDF (PC)

1030

摘要：

野生百合种类稀少，个别种类是中国特有，植物离体快速繁殖是保护稀少濒危百合种的有效方法，本研究为了探究一种朝鲜百合快速繁殖的方法。本研究以野生百合朝鲜为试材，以MS培养基为基础培养基，附加不同种类和浓度的植物生长调节物质（6-BA，NAA）诱导丛生芽及再生植株。以朝鲜百合的鳞茎为外植体，确定鳞茎的最佳消毒时间、影响鳞茎芽诱导的最佳激素组合以及不同激素对鳞茎芽增殖的影响。结果表明：最佳灭菌时间为0.1% HgCl消毒10 min；由于内源激素比例不同，鳞茎诱导的最佳培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L；最佳继代培养基为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L；最佳增殖培养基为MS+6-BA 1.0 mg/L+NAA 0.15 mg/L。

关键词: 大豆胞囊线虫, 大豆胞囊线虫, 生理小种, 致病性分化

Abstract:

Wild lily species is exiguous, individual species is unique to China, rapid propagation is an effective method to protect endangered or rare wild lily species. The purpose of this experiment was to develop a rapid propagation of Lilium amabile. This study used wild lily Lilium amabile as test materials, based on MS culture medium, additional different type and concentration of plant growth regulating substances (6-BA, NAA) induced adventitious bud and regenerative plants. With Lilium amabile bulb as explant, and determine the best sterilization time, bulbs affected bulb bud induction best hormone combination and different hormone of bulb buds proliferation influence. The results showed that the best disinfecting time was 0.1 % HgCl disinfection 10 minutes; Due to the different proportion endogenous hormones, the best bulb inductive medium: MS + 6-BA 1.0 mg/L + NAA 0.2 mg/L; the best subculture medium: MS + 6-BA 1.0 mg/L + NAA 0.1 mg/L; the best proliferation medium: MS + 6-BA1.0 mg/L + NAA 0.15 mg/L.