欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (5): 102-107.doi: 10.11924/j.issn.1000-6850.2014-2465

• 生物技术科学 • 上一篇    下一篇

黑龙江省核盘菌致病性分化研究

刘春来,王爽,夏吉星,杨帆,李新民,孟庆林,张匀华   

  1. 黑龙江省农业科学院植物保护研究所,黑龙江省农业科学院 植物保护研究所,黑龙江省农业科学院植物保护研究所,黑龙江省农业科学院 植物保护研究所,黑龙江省农业科学院植物保护研究所,黑龙江省农业科学院植物保护研究所,黑龙江省农业科学院植物保护研究所
  • 收稿日期:2014-09-11 修回日期:2015-02-04 接受日期:2014-10-14 出版日期:2015-03-20 发布日期:2015-03-20
  • 通讯作者: 张匀华
  • 基金资助:
    国家向日葵现代产业技术体系岗位专项资助项目（CARS-16）

Study on Pathogenicity Differentiation of Sclerotinia sclerotiorum Isolated from Different Regions in Heilongjiang Province

  • Received:2014-09-11 Revised:2015-02-04 Accepted:2014-10-14 Online:2015-03-20 Published:2015-03-20

摘要： 为进一步明确核盘菌种群内致病力分化现象，采用离体叶片菌饼接种方法，以向日葵品种‘丰葵杂1号’为供试寄主，对采集自黑龙江省6个地区18个县的大豆和向日葵两种寄主植物的93个核盘菌菌株进行了致病性测定。结果表明，除14个菌株不能致病外，其余79个菌株均能引起向日葵叶片发病，但所致病斑直径的大小差异很大(0.2~5.3 cm)，中等致病力菌株数量较多，共占总菌株数的66.7%。来源于同一地区以及同一地块的菌株其致病性也达到了极显著差异，说明在所测定的核盘菌分离株内确实存在着致病力强弱明显不同的菌株，这种致病力强弱明显不同的现象与菌株来源地区和寄主来源间均无明显的关系。

关键词: 平菇, 平菇, 重金属, 产量, 质量, 生物学效率

Abstract: In order to confirm pathogenicity differentiation within Sclerotinia sclerotiorum groups, ninety-three isolates of Sclerotinia sclerotiorum isolated from soybean and sunflower in different regions of Heilongjiang Province were tested for their pathogenicity to Fengkuiza NO1 variety by the method of placing the mycelia plugs on the excised leaves. The results of inoculation on excised leaves of Fengkuiza NO1 showed that 79 isolates had pathogenicity although the lesion sizes ranged from 0.2cm to 5.3cm in different isolates. However, no pathogenicity was found in fourteen isolates. The number of moderate pathogenicity isolates of Sclerotinia sclerotiorum were more than others and 66.7% proportion in the pathogen population. Furthermore, the extremely significant differences of pathogenicity were found among isolates even derived from the same region and the same field. The results indicated that the phenomenon of pathogenicity differentiation was existed among isolates, which was no significant relationship to geographical location and host origin of the isolates.