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中国农学通报

中国农学通报 ›› 2011, Vol. 27 ›› Issue (14): 87-93.

所属专题： 生物技术； 水产渔业

• 食品 营养 检测 安全 • 上一篇    下一篇

酶法制备鲽鱼鱼皮胶原蛋白肽及其清除超氧阴离子自由基的研究

王群 郑海涛 葛尧 何计国   

  • 收稿日期:2011-03-02 修回日期:2011-04-11 出版日期:2011-06-15 发布日期:2011-06-15

Study on the Enzymatic Preparation and Superoxide Anion Radical Scavenging Activity of Collagen Peptide from Plaice Skin

  • Received:2011-03-02 Revised:2011-04-11 Online:2011-06-15 Published:2011-06-15

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摘要：

采用碱性蛋白酶酶解鲽鱼鱼皮胶原蛋白制备胶原蛋白肽并对其清除超氧阴离子自由基的能力进行研究。通过单因素试验和响应面法分析不同酶解条件对超氧阴离子自由基清除率的影响，优化得到最佳酶解工艺条件：pH 9.5，加酶量1581 U/g，底物浓度10 mg/mL，酶解时间为6 h，酶解温度60℃，最高清除率为75.51%，半数清除浓度(IC50)为7.98 mg/mL。

关键词: 测土配方施肥, 测土配方施肥, flash, B/S, struts, hibernate

Abstract:

Alcalase was used to hydrolyze plaice skin for preparing collagen peptide, and the superoxide anion radical scavenging activity of this collagen peptide was studied. Effect of the different enzymatic hydrolysis conditions on superoxide anion radical scavenging activity were analyzed by single factor experiment and response surface method. The optimal hydrolytic conditions of plaice skin collagen using Alcalase with high scavenging rate were pH=9.5, enzyme dosage 1581 U/g, concentration of substrate 10 mg/mL, hydrolysis time 6 h，temperature 60℃. The maximum scavenging rate of 75.51% and half scavenging concentration (IC50) of 7.98 mg/mL were obtained.

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