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中国农学通报 ›› 2011, Vol. 27 ›› Issue (25): 77-81.

所属专题: 园艺

• 林学 园艺 园林 • 上一篇    下一篇

艾蒿提取液对茶尺蠖生物活性的初步研究

孙钦玉 高旭晖 杨云秋 袁争 张亮 朱尉   

  • 收稿日期:2011-03-23 修回日期:2011-05-01 出版日期:2011-10-05 发布日期:2011-10-05
  • 基金资助:

    国家自然科学基金

Biological Activities of Extracts from Artemisia argyi Against Ectropis obliqua

  • Received:2011-03-23 Revised:2011-05-01 Online:2011-10-05 Published:2011-10-05

摘要:

采用索氏提取法制取艾蒿(Artemisia argyi)乙醇粗提物并设定5个不同的浓度,观察其对茶尺蠖3龄幼虫的生物活性。结果表明,各处理对茶尺蠖3龄幼虫具有较强的触杀、胃毒活性和一定的拒食及抑制生长发育的作用,提取物浓度越高,效果越明显。当提取物在100 mg/mL浓度下,处理72 h后,触杀和胃毒活性中的校正死亡率分别高达66.14%、78.16%;此浓度下,12 h和24 h的拒食率分别达到了56.77%和46.01%;24 h和48 h的生长抑制率分别为54.83%和68.32%。数据显示:艾蒿乙醇粗提物对茶尺蠖具有较好的毒杀作用,有开发成植物源杀虫剂的潜力,符合当前无公害茶叶生产之急需。

关键词: 深山草莓, 深山草莓, 花瓣, 变异株系, 品种特性, 均匀设计

Abstract:

Preparation of the Artemisia argyi by Soxhlet extraction and set the alcohol extracts of five different concentrations, observation of the Artemisia argyi extracts were bio-assayed against Ectropis obliqua larva. Results indicated that the extracts had many functional patterns against the larvae of Ectropis obliqua as anti-feeding, contacting toxicity, stomach poisoning and inhibiting growth activities. The higher concentration, the more obviously the effect on the pest was. In the test of contacting toxicity and stomach poisoning, the corrected mortality of 3rd instar larvae of Ectropis obliqua reached 66.14% and 78.16% treated by contacting and poisoning at 72 h, respectively. For the anti-feeding, the results of the antifeedant effect showed that concentration of 100 mg/mL had good effect for the larva, the antifeedant effect were 56.77% and 46.01% after 12 h and 24 h, respectively. The extracts also had significant inhibition to growth and development of larvae the inhibition rate at 24 h and 48 h after treatment were 54.83% and 68.32% at concentration of 100 mg/mL. The date showed that Artemisia argyi extracts had good tea geometrid cytotoxicity, has developed into the potential of botanic insecticide, accord with current of non-polluted tea production needs.