关键词: 绵羊精子, 绵羊精子, 体外获能, 肝素, 咖啡因, 体外受精

Abstract:

In order to provide theoretical guidance for resistant disease breeding of peanut, resistant and susceptible cultivars of peanut were vaccinated by ralstonia solanacearum with different concentrations, the samples were collected in five periods, and enzymatic activities were measured by Spectrophotometer method. The activities of 4 enzymes in susceptible cultivars increased in earlier stage, but decline in later stage. Enzymatic activities of resistant cultivars increased slowly in earlier stage, but increased more and more rapidly in later stage. Not all of the 4 enzymatic activities of resistant cultivars were higher than that of susceptible cultivars in earlier stage, but all of enzymatic activities of resistant cultivars were higher than that of susceptible cultivars in later stage. Changes of enzymatic activities in the control groups were not obvious, and the differences of enzymatic activities in two cultivars were not significant too. PAL, CAT, PPO and POD as protective enzymes participated in the course reaction, their expression pattern differences were closely related with ralstonia solanacearum resistance of arachis hypogaea probablely.