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中国农学通报 ›› 2011, Vol. 27 ›› Issue (18): 84-89.

• 农学 农业基础科学 • 上一篇    下一篇

EMS诱变谷子‘长农35号’M1代成熟期株型突变体的鉴定与分析

王军 杨慧卿 袁峰 郭二虎   

  • 收稿日期:2011-03-02 修回日期:2011-04-28 出版日期:2011-07-25 发布日期:2011-07-25
  • 基金资助:

    EMS诱变长农35号谷子突变体库的建立与应用研究

Identification and Analysis of Mature Plant Type Mutants of M1 Generation of ‘Changnong35’, a Foxtail Millet Variety, Treated with EMS Mutagenesis

  • Received:2011-03-02 Revised:2011-04-28 Online:2011-07-25 Published:2011-07-25

摘要:

为建立谷子突变体库,以便在谷子分子生物学研究中应用,以生产上主要推广种植的谷子品种‘长农35号’干种子为实验材料,采用0.8%和1.0%甲磺酸乙酯(EMS)进行了诱变处理,试验共收获株型相关M1代突变体材料282份,其中,0.8% EMS诱变‘长农35号’获得株型突变体材料100个单株,可分为10个组;1.0% EMS诱变‘长农35号’获得株型突变体材料182个单株,可分为17个组。M1代成熟期株型相关性状突变体分析结果表明:1.0% EMS处理‘长农35号’所获突变体,在株高、穗下节粗、穗下第一节间粗和茎节数4个性状与对照有显著差异,而0.8%处理与对照差异不显著,因此,对于‘长农35号’来讲,采用1.0% EMS进行诱变处理更有利于多类型大量突变单株的获得。

关键词: 青稞, 青稞, 染色体, 核型分析

Abstract:

Foxtail millet cultivar ‘Changnong35’, which is widely used in agricultural production, was treated with EMS to establish foxtail millet mutant library for the molecular biology applications. We obtained 282 mutants in the M1 generation related to plant type, of which, 100 mutant plants obtained by using 0.8% EMS treatment can be divided into 10 groups; 182 mutant plants obtained by using 1.0% EMS treatment can be divided into 17 groups. The analysis results of the mature plant type traits of the M1 Generation showed that: plant height, stem diameter under spike, the diameter of the first internode and internode numbers treated with 1.0% EMS comparing with the control were significantly different, while the 0.8% EMS treatment comparing with control was not significant, therefore, the treatment of 1.0% EMS was more conducive to obtainment of a large number and multi-type mutants for ‘Changnong35’.

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