组织蛋白酶抑制剂E-64 对猪卵母细胞体外发育能力的影响

doi:10.11924/j.issn.1000-6850.casb15060123

中国农学通报 ›› 2015, Vol. 31 ›› Issue (32): 7-10.doi: 10.11924/j.issn.1000-6850.casb15060123

所属专题：生物技术；畜牧兽医

组织蛋白酶抑制剂E-64 对猪卵母细胞体外发育能力的影响

任小侠^1,2,马欣^1,2,梁琳²,常迪²,郭勇²,倪和民²

(¹中国兽医药品监察所,北京 100081；²北京农学院动物科技学院,北京 102206）

收稿日期:2015-06-24 修回日期:2015-08-14 接受日期:2015-08-25 出版日期:2015-11-16 发布日期:2015-11-16
通讯作者: 倪和民
基金资助:
2011 年度国家自然基金面上项目“不同来源植入前期绵羊胚胎IFNτ差异表达及其体外诱导子宫上皮细胞Galectin15 表达的研究”(31072185)；2012 年度国家科技重大专项“转基因生物新品种培育”一级子课题“抗病转基因羊扩繁体系初步建立”研究(2011ZX08008005-003)；北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”(IDHT 20130515)。

Effect of E-64 on Developmental Capacity of Porcine Oocytes in vitro

Ren Xiaoxia^1,2, Ma Xin^1,2, Liang Lin², Chang Di², Guo Yong², Ni Hemin²

(¹China Institute of Veterinary Drug Control, Beijing 100081; ²College of Animal Science and Technology, Beijing University of Agriculture, Beijing 102206)

Received:2015-06-24 Revised:2015-08-14 Accepted:2015-08-25 Online:2015-11-16 Published:2015-11-16

摘要/Abstract

摘要： 为探明外源性组织蛋白酶抑制剂(E-64)对猪卵母细胞体外发育能力的影响，通过向成熟液中添加1、5、10 μmol/L的外源性组织蛋白酶抑制剂，对猪卵丘-卵母细胞复合体(COCs)进行体外成熟培养 46 h，统计第一极体率，随后分别进行体外受精和孤雌激活，统计第2天的卵裂率和第7天的囊胚率。结果表明，成熟液中加入1 μmol/L E-64的第一极体排出率显著高于未添加的对照组以及5、10 μmol/L E-64的添加组。将体外成熟的卵母细胞进行体外受精及孤雌激活，体外受精后胚胎的卵裂率与囊胚率结果均为成熟液中加入1 μmol/L E-64的添加组显著高于对照组以及5、10 μmol/L E-64的添加组；孤雌激活后，4组间胚胎的卵裂率差异不显著，囊胚率为10 μmol/L E-64的添加组显著低于其他3组。因此 1 μmol/L E-64能促进猪卵母细胞的核成熟，并能进一步提高其体外受精后的卵裂率和体外培养的囊胚率。

关键词: 川北山区, 川北山区, 马铃薯, 开花, 产量, 研究

Abstract: The aim of this study was to ascertain the effect of exogenous cathepsin inhibitor (E-64) on the viability of porcine oocytes developed in vitro. The maturation medium was added with 1, 5, 10 μmol/L exogenous cathepsin inhibitors (E-64) respectively, and the porcine cumulus oocyte complexes (COCs) were matured in vitro for 46 hours, the first polar body rate was calculated. Then in vitro fertilization and parthenogenetic activation were conducted, and the cleavage rate and the blastocyst rate were analyzed. The results showed that the first polar body rate, as well as the associated cleavage rate and blastocyst rate in maturation medium added with 1 μmol/L E-64 were significantly higher than that of other three groups. After parthenogenetic activation, there was no significant difference in the cleavage rate among the four groups, while the blastocyst rate in maturation medium added with 10 μmol/L E-64 was significantly lower than that of other three groups. It could be concluded that the suitable supplement of E-64 for in vitro nuclear maturation of porcine oocytes, as well as the cleavage and embryonic development, should be 1 μmol/L.

任小侠,马欣,梁琳,常迪,郭勇,倪和民. 组织蛋白酶抑制剂E-64 对猪卵母细胞体外发育能力的影响[J]. 中国农学通报, 2015, 31(32): 7-10.

Ren Xiaoxia,Ma Xin,Liang Lin,Chang Di,Guo Yong and Ni Hemin. Effect of E-64 on Developmental Capacity of Porcine Oocytes in vitro[J]. Chinese Agricultural Science Bulletin, 2015, 31(32): 7-10.

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组织蛋白酶抑制剂E-64 对猪卵母细胞体外发育能力的影响

Effect of E-64 on Developmental Capacity of Porcine Oocytes in vitro

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