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中国农学通报 ›› 2011, Vol. 27 ›› Issue (28): 231-234.

• 林学 园艺 园林 • 上一篇    下一篇

特晚熟红桔JZT(P)-1胚珠败育的胚胎学研究

周心智 尤淑芬 张 卞德锡 杨晓红 张云贵   

  • 收稿日期:2011-04-19 修回日期:2011-06-15 出版日期:2011-11-05 发布日期:2011-11-05
  • 基金资助:

    国家现代农业产业(柑橘)技术体系建设专项资金资助(MATS)

Embryological Research on the Ovule Abortion Development of Super Late-maturing Cultivar JZT(P)-1 Tangerine

  • Received:2011-04-19 Revised:2011-06-15 Online:2011-11-05 Published:2011-11-05

摘要:

为进一步探明晚熟无核红桔的无核机理,为晚熟柑橘种质资源的开发利用提供可靠的科学依据,以特晚熟无核红桔JZT(P)-1为实验材料,采用直接解剖、连续石蜡切片、显微技术等方法重点对其胚珠败育过程进行系统的观察研究。结果表明,JZT(P)-1胚珠中的孢原细胞、胚囊原始细胞以及不同发育程度的胚囊基本上都败育,极少数胚珠发育成为健康的种子。在幼果前50天的发育中,胚囊原始细胞多起源发育,发生时间紊乱,使得珠心中早期孢原细胞及不同发育期胚囊的正常发育受到干扰,促使胚珠中各雌性结构的败育。幼果中雌性败育率极高,通过石蜡切片观察到正常胚的发育十分困难。基本成熟的果中,种子数0~4粒,平均种子数(1.3±0.42)粒,经济无核率高达100%。正常种子多胚。研究结果为探明特晚熟红桔新种质JZT(P)-1的无核机理提供了胚胎学证据。

关键词: 可持续发展, 可持续发展

Abstract:

To further prove the seedless mechanism on JZT(P)-1 and provide reliable scientific evidence to the exploitation of late-maturing Citrus germplasm, the ovule abortion process of Citrus reticulata, named JZT(P)-1, was systematically studied by a series of direct-dissection, consecutive paraffin sections and microscopic technique. It was found that the archesporial cell, embryo sac primitive cells and embryo sacs in different developing period were almost abortive. Only a few of seeds could develop into health seeds. During the 50 days of the development in the young seeds, archesporial cells developed from many origins and the occurrence time was out of order. That affected the normal development of early archesporial cells in the nucellar and embryo sacs in different developing periods. Thus, it spurred the abortion of the female structure in ovules. The rate of abortion in young fruits was very high, and it was difficult to observe the development of normal embryos by paraffin sections. There were 0-4 seeds in the nearly matured fruits and (1.3±0.42) seeds on an average. The economical seedlessness rate of the JZT(P)-1 was up to 100%. A few of normal seeds were polyembryonic. There were some embryos in a normal seed. The results in our research provided embryology basis to the ascertain of the extraordinary late maturing Citrus reticulate JZT(P)-1.