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中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (6): 182-186.doi: 10.11924/j.issn.1000-6850.2012-2711

所属专题： 生物技术； 水稻

• 生物技术科学 • 上一篇    下一篇

GLDH超表达水稻愈伤组织的耐盐性研究

刘拥海 俞乐 崔铁成 王若仲   

  • 收稿日期:2012-08-03 修回日期:2012-09-02 出版日期:2013-02-25 发布日期:2013-02-25
  • 基金资助:
    中国博士后科学基金“抗坏血酸缺失对水稻分蘖的影响”(2012M511723)；广东省自然科学基金“水稻叶片中抗坏血酸含量对其剑叶衰老及产量的影响”(S2011040001280)；肇庆市科技创新计划项目“肇实的种质鉴定与提纯复壮研究”（2011G17）；肇庆学院自然科学基金青年项目“农杆菌介导水稻GLDH基因导入结缕草的研究”(1003)，“水稻叶片中抗坏血酸含量对其剑叶衰老及产量的影响”(1105)；广东省自然科学基金“抗坏血酸缺失对水稻分蘖的影响”(S2012010010680)。

Study on Salt Tolerance of Callus in Rice with GLDH Gene Overexpressing

  • Received:2012-08-03 Revised:2012-09-02 Online:2013-02-25 Published:2013-02-25

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摘要： 为了探讨内源抗坏血酸(ascorbic acid，AsA)水平对水稻愈伤组织耐盐性的影响，以AsA合成关键酶半乳糖内酯脱氢酶(L-galactono-1,4-lactone dehydrogenase, GLDH)基因超表达型水稻(GO)为材料，研究了盐胁迫(1% NaCl)下GO愈伤组织继代生长及相关抗性生理指标的动态变化。结果表明，盐胁迫下GO愈伤组织生长明显优于野生型(wild type，WT)，表现为生长速率较快，愈伤颗粒较大，致密，呈浅黄色。盐胁迫下GO愈伤组织中还原型AsA与总AsA含量不断下降，DHA含量总体上也呈下降趋势，但其氧化还原势(AsA/DHA)在胁迫初期上升，后期快速下降。进一步研究发现盐胁迫下GO愈伤组织中MDA含量先上升后快速下降，但其含量显著低于WT，而POD活性在胁迫初期略有降低，在后期又快速升高，其活性总体上显著高于对照。因此上述研究表明GLDH超表达水稻愈伤组织的耐盐性增强与其AsA含量提高有关。

关键词: 紫色土, 紫色土

Abstract: In order to explore the effect of endogenous ascorbic acid levels on salt tolerance of callus in rice, dynamic changes of some resistance physiological indexes and growth of callus in transgenic rice (Oryza sativa L.) for L-galactono-1,4-lactone dehydrogenase (GLDH, EC 1.3.2.3) overexpressing (GO) under salt stress induced by 1% NaCl were studied. The results showed that growth of GO callus was significantly better than WT, such as faster growth rate, larger and looser callus particles, and pale yellow. Further research found that reduced AsA and total AsA content of GO callus decreased, the content of DHA in general also declined, while its redox potential (AsA/DHA ratio) first increased, late rapid declined under salt stress, while their contents were always higher than WT. MDA content of GO callus increased first and then decreased rapidly, and its content was significantly lower than that of WT. In addition, POD activity of GO callus slightly decreased slightly in early, and late rapidly increased, but its activity was always higher than that of WT. So these results suggested that enhancement of salt tolerance in GO callus was related to increase of AsA content.