欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (33): 291-295.doi: 10.11924/j.issn.1000-6850.2012-3448

所属专题： 油料作物

• 生物技术科学 • 上一篇    下一篇

花生不定芽分化及植株再生的研究

黄玲 岳增辉 缪蕾 赵凯 周静   

  • 收稿日期:2012-10-23 修回日期:2012-11-08 出版日期:2013-11-25 发布日期:2013-11-25

Study on Peanut Adventitious Shoots and Plant Regeneration

  • Received:2012-10-23 Revised:2012-11-08 Online:2013-11-25 Published:2013-11-25

PDF (PC)

553

摘要： 为进一步探索和完善高频植株再生体系并扩大基因型的研究，以8个花生品种为材料，对幼叶不同部位（叶片顶端、中间切段、叶片基部）的切段、子叶、上胚轴和下胚轴外植体，接种到添加1 mg/L NAA和6 mg/L BAP的MSB5培养基上，10天后将形成的愈伤组织转移至添加10 mg/L BAP或添加3 mg/L BAP和1 mg/L ABA的再分化培养基上进行培养，诱导不定芽分化。结果表明，6天叶龄叶片中间切段不定芽分化频率达到了91.4%，上胚轴26.7%，下胚轴12.5%和子叶0%；花生品种8823幼叶外植体获得了的91.4%不定芽分化率，生根植株经驯化后移至砂土中，正常开花结果。因此，幼叶外植体分化不定芽频率明显高于子叶、上胚轴和下胚轴；6天叶龄的叶片中间切段为最佳；不同基因型不定芽分化率存在明显差异。

关键词: 遗传分析, 遗传分析

Abstract: In order to perfect the plant regeneration system of tissue culture, a detailed study was carried out to provide the experimental basis for genetic transformation by using embryonic leaflet. The peanut plantlets were regenerated from immature leaves, cotyledon, epicotyl and hypocotyls on MSB5 medium supplemented with 1 mg/L NAA and 6 mg/L BAP. After 10 days, callus was transferred onto MSB5 medium supplemented with 10 mg/L BAP or 3 mg/L BAP and 1 mg/L ABA. The results were follows: the frequency of middle sections of 6 days explant was 91.4% in contrast to epicotyls 26.7% and hypocotyls 12.5%. The frequency of 8823 was up to 91.4%. The root was induced and the plant was generated. Conclusion: Compared with the cotyledon, epicotyl and hypocotyls, the immature leaves was the most easiest to induce adventitious shoots. The optimum explants source was the middle sections of 6 days. The frequency of adventitious shoots depended on genotypes.