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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (26): 66-70.doi: 10.11924/j.issn.1000-6850.casb15040014

所属专题： 生物技术； 小麦

• 生物技术科学 • 上一篇    下一篇

小麦花粉致死基因Ki的SSR标记分析

王 鹏1,2,张金文1,2,周宽基3,乔 岩1,2,王开芳1,2,王克婧1,2   

  1. (1甘肃省作物遗传改良与种质创新重点实验室/甘肃省干旱生境作物学重点实验室,兰州 730070；2甘肃农业大学农学院,兰州 730070；3甘肃省农业科学院小麦研究所,兰州 730070）
  • 收稿日期:2015-04-02 修回日期:2015-04-13 接受日期:2015-04-17 出版日期:2015-09-23 发布日期:2015-09-23
  • 通讯作者: 张金文
  • 基金资助:
    国家自然科学基金项目“特定普通小麦品种间杂种不育研究”(31060187)。

SSR Markers Analysis of Pollen Killing Gene Ki in Wheat

Wang Peng1,2, Zhang Jinwen1,2, Zhou Kuanji3, Qiao Yan1,2, Wang Kaifang1,2, Wang Kejing1,2   

  1. (1Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement/Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070;2College of Agronomy, Gansu Agricultural University, Lanzhou 730070;3Gansu Provincial Academy of Agricultural Sciences, Institute of Wheat, Lanzhou 730070)
  • Received:2015-04-02 Revised:2015-04-13 Accepted:2015-04-17 Online:2015-09-23 Published:2015-09-23

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摘要： 小麦雄性不育主要是通过花粉的败育表现，其不育材料对小麦杂种优势的利用研究具有重要意义和价值，国外研究表明，某些特定普通小麦品种间杂交F1表现的花粉部分不育现象，受控于核基因组花粉致死基因Ki，为了筛选小麦花粉致死基因Ki的连锁标记，利用现代分子生物学技术通过定位该基因，克隆出花粉致死基因连锁标记片段，为小麦雄性不育种质材料的转育提供有效的选择标记。对小麦花粉致死基因Ki进行了分子标记定位，以‘中国春’和澳大利亚春小麦品种的BC1F1代作为定位群体，利用分离群体分组分析法(BSA)对位于小麦6B染色体上85对SSR引物进行多态性筛选，具有多态性的引物再通过BC1F1定位群体进行验证，从中筛选出与目的基因连锁的2个SSR标记Xgwm626和Xgpw4138。运用Mapmaker 3.0软件进行连锁分析。结果表明，Xgwm626和Xgpw4138与Ki基因的遗传距离分别为9.2 cM和6.9 cM，且2个SSR标记位于目的基因两侧，并将Ki定位于小麦6BL染色体上。研究结果为Ki基因的分子标记辅助选择和进一步精细定位奠定了基础。

关键词: 铅锌矿区, 铅锌矿区, 重金属污染, 植物群落, 物种多样性

Abstract: Wheat sterile mainly expressed through the performance of pollen abortion. The study on infertility material of wheat heterosis has great significance. Overseas research shows that among certain parts of the common wheat hybrids F1, pollen sterility is controlled by nuclear genome of pollen killing gene Ki. In order to screen linked markers of pollen killing gene Ki in wheat, modern molecular biology techniques were used to locate the gene, clone fragment pollen lethal gene linked markers, provide an effective selection marker for transformation of male sterility in wheat germplasm and study the molecular markers of pollen killing gene Ki in wheat. A BC1F1 population derived from the crossing between Chinese spring wheat and Australian spring wheat was used for molecular mapping of Ki gene. The sterile and fertile near isogenic bulks was constructed from the above BC1F1 population. By using Bulked Segregant Analysis (BSA) method, 85 SSR primers on wheat 6B chromosome were screened, and two SSR markers (Xgwm626 and Xgpw4138) were found to be linked to Ki gene. These markers could be used in fine mapping of Ki and marker-assisted selection. Two SSR markers Xgwm626 and Xgpw4138 located on the long arm of chromosome 6B were linked to Ki, and their genetic distances to Ki were 9.2 cM and 6.9 cM, respectively, indicating that these two markers could be used in molecular-assisted breeding.