铜、锰诱导吉富罗非鱼血细胞凋亡及铁、锌的干预作用

doi:10.11924/j.issn.1000-6850.casb15050049

中国农学通报 ›› 2015, Vol. 31 ›› Issue (35): 58-65.doi: 10.11924/j.issn.1000-6850.casb15050049

所属专题：生物技术；水产渔业

铜、锰诱导吉富罗非鱼血细胞凋亡及铁、锌的干预作用

白丽蓉,赵志英

海南省海洋与渔业科学院,海南省海洋与渔业科学院

收稿日期:2015-05-11 修回日期:2015-11-17 接受日期:2015-08-25 出版日期:2015-12-18 发布日期:2015-12-18
通讯作者: 赵志英
基金资助:
海南省科学事业费项目“海水养殖对海南沿岸生态环境影响及控制措施研究”(11-201410-0009)。

Effects of Copper and Manganese on Hemocyte Apoptosis and the Antagonism of Iron and Zinc in Oreochromis nilotcus

Received:2015-05-11 Revised:2015-11-17 Accepted:2015-08-25 Online:2015-12-18 Published:2015-12-18

摘要/Abstract

摘要： 为了研究重金属铜与锰对吉富罗非鱼血细胞凋亡的影响以及铁、锌的拮抗作用，采用原子吸收分光光度法检测血液及饲料中重金属的含量，采用流式细胞术检测实验鱼血细胞凋亡情况。360 尾吉富罗非鱼幼鱼随机分为12 组，每组30 尾，分别以硫酸铜(0、2000 mg/kg)、硫酸锰(0、120 mg/kg)为攻毒组重金属源，以硫酸锌(20、320 mg/kg)、硫酸铁(150、350 mg/kg)为拮抗组金属源，通过饲料投喂的方式进行血细胞凋亡的研究，养殖周期为20 周。细胞凋亡分析结果表明，染铜组、染锰组实验鱼血细胞凋亡率显著高于对照组；补充铁和锌后，随着饲料添加铁、锌水平的增加，实验鱼血细胞凋亡率明显下降，但铁、锌干预组血细胞凋亡率显著高于对照组。实验表明，过量铜、锰可诱发实验罗非鱼血细胞凋亡；较高水平的铁、锌对铜、锰的毒性作用具有拮抗作用。

关键词: 土壤水分, 土壤水分, 热惯量法, 能量指数法, 植被供水指数法

Abstract: The objective of this study was to investigate the influence of copper and manganese on apoptosis in blood cells of Oreochromis nilotcus and the antagonistic effect of iron and zinc. Atomic absorption spectrophotometry was used to analyze heavy mental content in fish blood and food, and flow cytometry was applied to analyze fish blood cell apoptosis. Juvenile Tilapia were randomly divided into 12 groups with 30 fish in each group. The challenge groups were fed with constant level of copper sulfate (0, 2000 mg/kg) and manganese sulfate (0, 120 mg/kg) diet, and the antagonized groups were fed with zinc sulfate (20, 320 mg/kg) and iron sulfate (150, 350 mg/kg). Twenty weeks later, the number of apoptotic cells in the blood obviously increased in copper group and manganese group. After adding iron and zinc, the number of apoptotic cells was lower than that of the copper group and manganese group. The numbers of apoptotic cells in the iron and zinc interventional groups were significantly higher than that in the control group. Copper and manganese can cause apoptosis in blood cells. However, those effects can be antagonized by iron and zinc.

中图分类号:

Q952

白丽蓉,赵志英. 铜、锰诱导吉富罗非鱼血细胞凋亡及铁、锌的干预作用[J]. 中国农学通报, 2015, 31(35): 58-65.

参考文献

[1] Stabel J R, Spears J W. Effect of copper on immune function and disease resistance[J]. Advances in experimental medicine and biology, 1989, 258: 243-252.
[2] Schrantz N, Blanchard D A, Mitenne F, et al. Manganese induces apoptosis of human B cells: caspase-dependent cell death blocked by bcl-2[J]. Cell death and differentiation, 1999, 6(5): 445-453.
[3] Jacob A K, Hotchkiss R S, DeMeester S L, et al. Endothelial cell apoptosis is accelerated by inorganic iron and heat via an oxygen radical dependent mechanism[J]. Surgery, 1997, 122(2): 243-254.
[4] Brockhaus F, Brne B. Overexpression of CuZn superoxide dismutase protects RAW 264.7 macrophages against nitric oxide cytotoxicity[J]. Biochemical Society, 1999, 338(Pt 2): 295-303.
[5] Ishido M, Suzuki T, Adachi T, et al. Zinc stimulates DNA synthesis during its antiapoptotic action independently with increments of an antiapoptotic protein, Bcl-2, in porcine kidney LLC-PK(1) cells[J]. The journal of pharmacology and experimental therapeutics, 1999, 290(2): 923-928.
[6] 李萡,王枫. 锌对细胞凋亡调控研究的一些思考[J]. 医学与哲学,2000,21(4): 19-21.
[7] Kerr J, Wyllie A H, Currie A R. Apoptosis: a basic biological phenomenon with wide-tanging implications in tissue kinetics[J]. Cancer, 1972, 26: 239-257.
[8] 马晓娟,王宇. 超声诱导细胞凋亡的研究与进展[J]. 中国临床康复,2005,9(15): 150-151.
[9] McConkey D J, Orrenius S. Signal transduction pathways to apoptosis[J]. Trends in cell biology, 1994, 4(10): 370-375.
[10] 刘学东,郑冬,关伟军. 细胞凋亡检测方法研究[J]. 上海畜牧兽医通讯,2007,(2): 52-54.
[11] HaMai D, Campbell A, Bondy S C. Modulation of oxidative events by multivalent manganese complexes in brain tissue[J]. Free radical biology medicine, 2001, 31(6): 763-768.
[12] Malecki E A. Manganese toxicity is associated with mitochondrial dysfunction and DNA fragmentation in rat primary striatal neurons[J]. Brain research bulletin, 2001, 55(2): 225-228.
[13] Desole MS, Sciola L, Delogu MR, et al. Manganese and 1-methy1-4-(2’-ethylphenyl)-1,2,3,6-etrahydropyridine induce apoptosis in PC12 cells[J]. Neuroscience Letters, 1996, 209(3): 193-196.
[14] 吴萍,张杰,李洁,等. 锰对神经细胞凋亡和细胞周期的影响及硒的保护作用[J].卫生毒理学杂志,2004,18(4): 272-274.
[15] 吉顺福,樊卫萍. 锰诱导小鼠肝、脑细胞凋亡及锌的干预作用[J]. 现代预防医学,2009,36(23): 4540-4546.
[16] Perry D K, Smyth M J, Stennicke H R, et al. Zinc is a potent inhibitor of the apoptotic protease, caspase-3[J]. The Journal of Biological Chemistry, 1997, 272(30): 18530-18533.
[17] Torriglia A, Chaudun E, Courtois Y, et al. On the use of Zn2 to discriminate endonucleases activated during apoptosis[J]. Biochimie, 1997, 79(7): 435-438.
[18] Duan H, Wang Y, Aviram M, et al. SAG, a novel zinc RING finger protein that protects cells from apoptosis induced by redox agents[J]. Molecular and cellular biology, 1999, 19(4): 3145-3155.
[19] 马得莹,单安山. 铜对动物体内自由基防御系统酶活性及其基因表达的影响[J]. 中国畜牧兽医,2003,30(5): 27-29.
[20] 胡洁,蔡真. 细胞器相关凋亡途径研究进展[J]. 中国肿瘤生物治疗杂志,2005,12(2): 152-154.
[21] Palmer A M, Greengrass P M, Cavalla D. The role of mitochondria in apoptosis[J]. Drug news perspectives, 2000, 13(6): 378-384.
[22] Fridman J S, Lowe S W. Control of apoptosis by p53[J]. Oncogene, 2003, 22(56): 9030-9040.
[23] Kovar J, Stunz L L, Steward B C, et al. Direct evidence that iron deprivation induces apoptosis in murine lymphoma 38C13[J]. Pathobiology, 1997, 65(2): 61-68.
[24] Hamada K, Takahashi I, Matsuoka M, et al. Apoptosis of peripheral leukocytes in patients with myelodysplastic syndromes[J]. The Japanese Journal of Clinical Hematology, 1998, 39(11): 1097-84.
[25] Vdlez-Pardo C, De1 Rio M J, Ebinger G, et al. Monoamine and iron-related toxicity: from“serotonin-binding proteins”to lipid peroxidation and apoptosis in PC12 cells[J]. General Pharmacology: The Vascular System, l998, 31(1): 19-24.
[26] 赵卫红,陈丽君. 铁对过氧化氢诱导细胞凋亡的保护作用研究[J]. 中国小儿血液,2002,7(1): 6-7.
[27] 赵卫红,陈家佩,从玉文,等. 辐射诱发肿瘤细胞增殖抑制和凋亡的相关研究[J]. 中华放射肿瘤学杂志,1999,(2): 100-102.

铜、锰诱导吉富罗非鱼血细胞凋亡及铁、锌的干预作用

Effects of Copper and Manganese on Hemocyte Apoptosis and the Antagonism of Iron and Zinc in Oreochromis nilotcus

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