一株抑草真菌发酵液中的抑草成分分析

doi:10.11924/j.issn.1000-6850.casb20200200088

中国农学通报 ›› 2020, Vol. 36 ›› Issue (35): 106-112.doi: 10.11924/j.issn.1000-6850.casb20200200088

所属专题：生物技术

一株抑草真菌发酵液中的抑草成分分析

张奇¹^,²(), 张清旭², 庞晓敏¹^,², 王海斌²^,³, 叶江华¹^,², 贾小丽¹^,², 何海斌²()

¹武夷学院茶与食品学院,福建武夷山 354300
²福建省农业生态过程与安全监控重点实验室,福建农林大学,福州 350002
³龙岩学院生命科学学院,福建龙岩 364012

收稿日期:2020-02-05 修回日期:2020-04-09 出版日期:2020-12-15 发布日期:2020-12-18
通讯作者: 何海斌
作者简介:张奇,男,1989年出生,福建莆田人,讲师,博士,研究方向：化学生态学。通信地址：354300 福建省南平市武夷山市武夷学院,Tel：0599-5137553,E-mail： 1185458937@qq.com。
基金资助:
国家自然科学基金“稗草根系分泌物中水稻化感作用诱导成分分析及其诱导机理研究”(31370380);“化感水稻抑草圈形成的土壤微生物变化与调控机制研究”(31070447);福建省教育厅中青年教师教育科研项目“外源诱导提高水稻化感的作用机理研究”(JAT190786)

Herbicide Ingredients in Fermentation Broth of One Strain of Fungi: Analysis

Zhang Qi¹^,²(), Zhang Qingxu², Pang Xiaomin¹^,², Wang Haibin²^,³, Ye Jianghua¹^,², Jia Xiaoli¹^,², He Haibin²()

¹School of Tea and Food Science, Wuyi University, Wuyishan Fujian 354300
²Fujian Provincial Key Laboratory of Agroecological Processing and Safety Monitoring, Fujian Agriculture and Forestry University, Fuzhou 350002
³College of Life Sciences, Longyan University, Longyan Fujian 364012

Received:2020-02-05 Revised:2020-04-09 Online:2020-12-15 Published:2020-12-18
Contact: He Haibin

摘要/Abstract

摘要：

为了鉴定从桉树根际筛选到的微生物的抑草潜力和抑草成分,以桉树根际土壤中筛选到的一株真菌为材料,利用生物测试验证菌株发酵液的抑草潜力,利用GC-MS对菌株发酵液抑草成分进行验证。结果表明,不同浓度菌株发酵液对稗草的根长、株高和干重都有显著的抑制作用,对稗草根长抑制率最大为稀释50倍时,抑制率为7.55%;对稗草株高和干重抑制率最高为原液,抑制率分别为56.53%和25.46%。GC-MS鉴定共检测到86个物质,含氮化合物最多,占59.10%,其次为羧酸及酯类化合物,占19.26%。抑草物质生物测试结果表明,邻香豆酸、柠檬醛和胡椒碱在低浓度时对莴苣的根长和株高都有很强的抑制作用。表明该菌株能够产生多种化感物质的结构类似物,使得菌株发酵液具有很强的抑草作用,具有开发为微生物除草剂的潜力。

关键词: 桉树, 抑草真菌, 发酵液, 化感物质, GC-MS

Abstract:

To identify the herbicidal potential and constituents of microorganisms from Eucalyptus rhizosphere, a strain of fungi from the rhizosphere soil of Eucalyptus was used as material. The herbicidal activity of the strain fermentation broth was identified by bioassay, and the components of fermentation broth were further verified by GC-MS. The results showed that the fermentation broth of the strain at different concentrations had significant inhibition on the root length, plant height and dry weight of barnyard grass. The inhibition rate of the fermentation broth on the root length of barnyard grass was the highest (7.55%) under 50 times of diluted solution. The highest inhibition rate of barnyard grass height and dry weight was 56.53% and 25.46% by undiluted fermentation broth, respectively. GC-MS results showed that a total of 86 substances were detected, of which the largest proportion of compounds was nitrogen compounds accounting for 59.10%, followed by carboxylic acids and esters accounting for 19.26%. The bioassay results showed that o-coumaric acid, citral and piperine could inhibit the root length and plant height of lettuce at low concentrations. These results indicated that the strain could produce a variety of allelochemical analogues compounds, which made the strain fermentation broth have a strong weed-inhibition, and a great potential to be developed as a microbial herbicide.

Key words: Eucalyptus, weed-inhibiting fungi, fermentation broth, allelochemical, GC-MS

中图分类号:

S181

张奇, 张清旭, 庞晓敏, 王海斌, 叶江华, 贾小丽, 何海斌. 一株抑草真菌发酵液中的抑草成分分析[J]. 中国农学通报, 2020, 36(35): 106-112.

Zhang Qi, Zhang Qingxu, Pang Xiaomin, Wang Haibin, Ye Jianghua, Jia Xiaoli, He Haibin. Herbicide Ingredients in Fermentation Broth of One Strain of Fungi: Analysis[J]. Chinese Agricultural Science Bulletin, 2020, 36(35): 106-112.

图/表 4

参考文献 30

[1]	Westwood J H, Charudattan R, Duke S O, et al. Weed management in 2050: perspectives on the future of weed science[J]. Weed Science, 2018,66(3):275-285. doi: 10.1017/wsc.2017.78 URL
[2]	景小兰, 董良利, 张建华, 等. 中国除草剂的发展现状及其在高粱上的应用[J]. 中国农学通报, 2015,31(25):182-185.
[3]	黄顶成, 尤民生, 侯有明, 等. 化学除草剂对农田生物群落的影响[J]. 生态学报, 2005,25(6):221-228.
[4]	姚路加. 生物除草剂发展仍需时日[J]. 农药市场信息, 2015(17):38.
[5]	尹会会, 杨中旭, 李秋芝, 等. 化学除草剂研发与应用中存在的问题及解决对策[J]. 山东农业科学, 2013,45(4):126-129.
[6]	谢阳军. 抑制稗草微生物菌株A的鉴定及机理研究[D]. 福州:福建农林大学, 2010.
[7]	曾秘, 张亚, 彭争科, 等. 微生物除草剂的研究现状[J]. 江西农业学报, 2013(2):40-43,46.
[8]	赵绍文, 王凌晖, 蒋欢军, 等. 巨尾桉枝叶水浸提液对3种作物种子萌发的影响[J]. 广西科学院学报, 2000(1):14-17.
[9]	Bajwa R, Nazi I. Allelopathic effects of Eucalyptus citriodora on growth, nodulation and AM colonization of Vigna radiata (L) Wilczek[J]. Allelopathy Journal, 2005,15(2):237-246.
[10]	Florentine S K, Fox J E D. Allelopathic effects of Eucalyptus victrix L. on Eucalyptus species and grasses[J]. Allelopathy Journal, 2003,11(1):77-84.
[11]	赵善欢. 植物化学保护[M]. 北京: 中国农业出版社, 2000.
[12]	陈尧, 张奇, 姜帅, 等. 桉树根际土壤抑草放线菌的筛选与鉴定[J]. 亚热带农业研究, 2018,14(2):110-115.
[13]	张奇, 胡文文, 林晓丹, 等. 桉树根区土壤中抑草真菌的筛选分离[J]. 福建农林大学学报:自然科学版, 2015,44(2):159-164.
[14]	李家玉, 林志华, 李立, 等. 化感水稻苗期茎叶组织中萜类物质检测和抑草活性分析[J]. 福建农林大学学报:自然科学版, 2017,46(2):129-134.
[15]	马娟, 董金皋. 微生物除草剂与生物安全[J]. 植物保护, 2006,32(1):9-12.
[16]	曾秘, 张亚, 彭争科, 等. 微生物除草剂的研究现状[J]. 江西农业学报, 2013(2):44-47,50.
[17]	杨恒友, 张剑, 刘杰. 微生物除草剂研究现状与展望[J]. 北方园艺, 2010(14):216-218.
[18]	杨云强, 姜述君, 范文艳, 等. 利用复合微生物除草剂控制稗草和反枝苋的研究[J]. 黑龙江八一农垦大学学报, 2009,21(4):34-37.
[19]	杨文权, 慕小倩, 安德荣. 微生物除草剂开发的限制因素和对策[J]. 农药, 2003,42(8):8-9.
[20]	Kremer R J, Souissi T. Cyanide production by rhizobacteria and potential for suppression of weed seedling growth[J]. Current microbiology, 2001,43(3):182-186. doi: 10.1007/s002840010284 URL
[21]	Kremer R J, Kennedy A C. Rhizobacteria as biocontrol agents of weeds[J]. Weed Technology, 1996,10(3):601-609. doi: 10.1017/S0890037X00040525 URL
[22]	Ōtake N. Recent progress in research on agricultural antibiotics in Japan[M]// Natural Products. Pergamon, 1983:3-10.
[23]	Li J Y, Zhang Q, Li L, et al. Isolation and characterization of Penicillium decumbens from the rhizosphere soil of allelopathic rice as mycoherbicide against Barnyard grass[J]. Allelopathy Journal, 2018,44(1):13-24. doi: 10.26651/AJ URL
[24]	谢阳军, 林漩, 胡开辉. 1株抑稗草微生物的鉴定及其抑草机理分析[J]. 福建农业科技, 2015(7):16-22.
[25]	张奇, 梁友谊, 胡林文文, 等. 从化感水稻根际土壤中筛选抑草细菌的研究[J]. 中国农学通报, 2015,31(15):179-183.
[26]	张奇, 梁友谊, 胡文文, 等. 稻田土壤中抑草真菌的筛选与鉴定[J]. 中国农学通报, 2015,31(12):210-216.
[27]	Li J Y, Lin S X, Zhang Q, et al. Phenolic acids and terpenoids in the soils of different weed-suppressive circles of allelopathic rice[J]. Archives of Agronomy and Soil Science, 2020,66(2):266-278. doi: 10.1080/03650340.2019.1610560 URL
[28]	Kruse M, Strandberg M, Strandberg B. Ecological effects of allelopathic plants-a review[J]. NERI Technical Report, 2000,315.
[29]	Müller C. Role of glucosinolates in plant invasiveness[J]. Phytochemistry Reviews, 2009,8(1):227-242. doi: 10.1007/s11101-008-9116-2 URL
[30]	林漩. 化感水稻根际土壤中抑草功能的微生物的筛选及其特性的研究[D]. 福州:福建农林大学, 2007.

s	TM1	TM2	TM3	TM4
根长	5.02±0.45b	2.15±0.18c	5.81±0.23b	7.55±0.32a
株高	56.53±4.86a	46.97±4.32bc	51.02±2.35b	42.73±6.18c
干重	25.46±1.16a	17.17±3.09bc	22.53±2.10b	10.38±2.86c

s	TM1	TM2	TM3	TM4
根长	5.02±0.45b	2.15±0.18c	5.81±0.23b	7.55±0.32a
株高	56.53±4.86a	46.97±4.32bc	51.02±2.35b	42.73±6.18c
干重	25.46±1.16a	17.17±3.09bc	22.53±2.10b	10.38±2.86c

类别	化合物名称	分子式	相对含量/%
含氮类化合物	Alanine	C₃H₇NO₂	1.02
	Proline, 5-oxo-, methyl ester	C₆H₉NO₃	0.78
	1-Hexanamine	C₆H₁₅N	1.39
	Cyclohexanamine, 5-methyl-2-(1-methylethyl)-	C₁₀H₂₁N	1.35
	3-Hydroxyethyl aniline	C₈H₁₁NO	0.78
	Butanamide, 3-methyl-	C₅H₁₁NO	1.16
	Hexadecanamide	C₁₆H₃₃NO	0.63
	Cyclopentanecarboxamide,3-ethenyl-2-(3-pentenylidene)-N-phenyl-	C₁₉H₂₃NO	1.12
	Ethanimidic acid, ethyl ester	C₄H₁₀ClNO	1.03
	1,3,4-Thiadiazol-2- amino	C₂H₃N₃S	0.50
	1,2,4-Thiadiazole, 5-amino-	C₂H₃N₃S	0.56
	Propanenitrile, 3-(methylthio)-	C₄H₇NS	5.84
	2-Amino-9-fluorenone	C₁₃H₉NO	0.86
	Thiophene-2-carboxylic acid, [3,5-dimethyl-1- (thiophene-2-carbonyl)-1H-pyrazol-4-yl]amide	C₁₅H₁₃N₃O₂S₂	0.37
	Tranexamic acid	C₈H₁₅NO₂	2.09
	7H-Dibenzo(a,g)carbazole	C₂₀H₁₃N	0.64
	6-Benzamido-4-benzoyl-1,2,4-triazine-3,5(2H,4H)-dione	C₁₇H₁₂N₄O₄	0.93
	Thiazole	C₃H₃NS	0.78
	Phenol, 2-amino-5-methyl-	C₇H₉NO	2.02
	Phenylephrine	C₉H₁₃NO₂	1.92
	Phenol, 2-(2-furyl)(2-pyrimidylamino)methyl-	C₁₅H₁₃N₃O₂	0.47
	5-Amino-7-(4-methoxy-phenyl)-2-phenyl-pyrazolo[1,5-a]pyrimidine-6-carbonitrile	C₂₀H₁₅N₅O	0.50
	1H-Imidazole-1-ethanol,-methyl-	C₆H₁₀N₂O	3.88
	2,5-Pyridinedicarboxylic acid	C₇H₅NO₄	0.98
	4,6-Diamino-O-cresol	C₇H₁₀N₂O	0.43
	1,2,3-Benzothiadiazol-5-ol	C₆H₄N₂OS	0.49
	2,4-Dimethoxy-5-pyrimidine carboxaldehyde	C₇H₈N₂O₃	1.20
	9H-Pyrido[3,4-b]indole	C₁₁H₈N₂	1.62
	Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-	C₇H₁₀N₂O₂	5.20
	2,4-Dihydroxypyridine	C₅H₅NO₂	1.58
	Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-	C₁₁H₁₈N₂O₂	0.68
	4,4-Dimethyl-2,5-dioxo-1-vinylimidazolidine	C₇H₁₀N₂O₂	0.86
	Chlorpropham	C₁₀H₁₂ClNO₂	0.69
	Cyclohexanecarbonitrile, 4-oxo-1-phenyl-	C₁₃H₁₃NO	0.51
	1,3-Benzodioxole-5-carboxylic acid, 6-acetamino-	C₁₀H₉NO₅	0.69
	Butyric acid, 4-(2-carbamoylpyrrolidin-1-yl)-4-oxo-	C₉H₁₄N₂O₄	1.05
	3,6-Diisopropyl-2,5-dioxomorpholine	C₁₀H₁₇NO₃	0.77
	4-Aminoresorcinol	C₆H₇NO₂	0.72
	Isoproterenol tri-TMS derivative	C₁₁H₁₇NO₃	0.66
	Thiophene-2-carboxylic acid, 4,5-dimethyl-3-(1-pyrrolyl)-	C₁₁H₁₁NO₂S	2.52
	Ergosta-7,22-dien-6-one, 3-hydroxy-, O-methyloxime	C₂₉H₄₇NO₂	1.94
	Indazol-4-one, 5,6-dimethyl-2-phenyl-3-pyridin-3-yl -2,5,6,7-tetrahydro-	C₂₀H₁₉N₃O	1.56
	1H-Carbazole-9-ethanol, 2,3,4,9-tetrahydro-[[(1-methyl-1H-imidazol-2-yl)thio]methyl]-	C₁₉H₂₃N₃OS	1.24
	1-Benzo[1,3]dioxol-5-ylmethyl-6,7-dimethoxy-2-nitroso-1,2,3,4-tetrahydroisoquinolin-4-ol	C₁₉H₂₀N₂O₆	0.42
	3-Methyl-butyric acid, 4-butyl-2-methyl-5-oxotetrahydrofuran-3-yl ester	C₁₃H₁₉NO₃	0.57
	Carbamic acid, methyl-, phenyl ester	C₈H₉NO₂	0.68
	Furo[3,4-c]pyridin-1(3H)-one, 7-hydroxy-6-methyl-	C₈H₇NO₃	0.55
	Acetic acid, cyano-, 2-methoxyethyl ester	C₆H₉NO₃	0.87
	小计		59.10
香草醛或香豆素类化合物	Vanillin	C₈H₈O₃	1.71
	Benzaldehyde, 3,4-dihydroxy-5-methoxy-	C₈H₈O₄	0.91
	Benzaldehyde, 2,5-dimethoxy-	C₉H₁₀O₃	1.33
	Benzaldehyde, 2-hydroxy-3-methoxy-	C₈H₈O₃	1.43
	8-Hydroxycoumarin	C₉H₆O₃	1.25
	3-n-Butyrylcoumarin	C₁₃H₁₂O₃	0.56
	小计		7.19
羧酸及酯类化合物	4-Acetylbutyric acid	C₆H₁₀O₃	1.60
	4-Acetylbenzoic acid	C₉H₈O₃	0.89
	Crotonic acid	C₄H₆O₂	2.96
	4-Acetoxycinnamic acid	C₁₁H₁₀O₄	1.47
	3-Phenylpropionic acid, 5-methoxy-2-[3,4-dimethoxyphenyl]-	C₁₈H₂₀O₅	1.63
	Propionylfilicinic acid	C₁₁H₁₄O₄	0.86
	3-(3-Hydroxyphenyl)propionic acid	C₉H₁₀O₃	1.01
	Protocatechuic acid	C₇H₆O₄	3.09
	3,5-di-tert-Butyl-4-hydroxyphenylpropionic acid	C₁₇H₂₆O₃	0.63
	Dodecanoic acid, 2-methyl-	C₁₃H₂₆O₂	0.51
	Methyl 4-methoxysalicylate	C₉H₁₀O₄	0.55
	Benzoic acid, 2-hydroxy-, pentyl ester	C₁₂H₁₆O₃	0.88
	Benzoic acid, 2,3,4-trihydroxy-, methyl ester	C₈H₈O₅	1.12
	1-Butanol, 3-methyl-, acetate	C₇H₁₄O₂	0.77
	1,5-Hexadiene-3-carboxylic acid, 1-methoxy-5-methyl-, methyl ester	C₁₀H₁₆O₃	0.59
	Octadecanoic acid, methyl ester	C₁₉H₃₈O₂	0.70
	小计		19.26
酮类化合物	1-Indanone, 5,6-dimethyl-	C₁₁H₁₂O	0.72
	2,6-Dihydroxyacetophenone	C₈H₈O₃	0.93
	2,5-Dihydroxypropiophenone	C₉H₁₀O₃	1.38
	2-Pentanone, 4-hydroxy-4-methyl-	C₆H₁₂O₂	1.00
	7,7-Bis-hydroxymethyl-1,4-dioxaspiro[4.5]decan-8-one	C₁₀H₁₆O₅	0.83
	小计		4.86
醇（酚）类化合物	3,3-Dimethyl-2-pentanol	C₇H₁₆O	0.60
	1,2-Propanediol, 3-methoxy-	C₄H₁₀O₃	1.14
	2-Propylcyclohexanol	C₉H₁₈O	0.46
	(2,3-Dimethyloxiranyl) methanol	C₅H₁₀O₂	1.28
	Vinylfuran	C₆H₆O	0.93
	1-(2-Furyl)-3-buten-1-ol	C₈H₁₀O₂	0.57
	1,4-Benzenediol, 2,3,5-trimethyl-	C₉H₁₂O₂	0.93
	trans-4-Methyl-5-methoxy-1-(1-hydroxy-1-isopropyl)cyclohex-3-ene	C₁₁H₂₀O₂	0.47
	2(R),3(S)-1,2,3,4-Butanetetrol	C₄H₁₀O₄	1.05
	小计		7.43
Citral		C₁₀H₁₆O	1.57
3H-Benz(e)indene, 2-methyl-		C₁₄H₁₂	0.60

类别	化合物名称	分子式	相对含量/%
含氮类化合物	Alanine	C₃H₇NO₂	1.02
	Proline, 5-oxo-, methyl ester	C₆H₉NO₃	0.78
	1-Hexanamine	C₆H₁₅N	1.39
	Cyclohexanamine, 5-methyl-2-(1-methylethyl)-	C₁₀H₂₁N	1.35
	3-Hydroxyethyl aniline	C₈H₁₁NO	0.78
	Butanamide, 3-methyl-	C₅H₁₁NO	1.16
	Hexadecanamide	C₁₆H₃₃NO	0.63
	Cyclopentanecarboxamide,3-ethenyl-2-(3-pentenylidene)-N-phenyl-	C₁₉H₂₃NO	1.12
	Ethanimidic acid, ethyl ester	C₄H₁₀ClNO	1.03
	1,3,4-Thiadiazol-2- amino	C₂H₃N₃S	0.50
	1,2,4-Thiadiazole, 5-amino-	C₂H₃N₃S	0.56
	Propanenitrile, 3-(methylthio)-	C₄H₇NS	5.84
	2-Amino-9-fluorenone	C₁₃H₉NO	0.86
	Thiophene-2-carboxylic acid, [3,5-dimethyl-1- (thiophene-2-carbonyl)-1H-pyrazol-4-yl]amide	C₁₅H₁₃N₃O₂S₂	0.37
	Tranexamic acid	C₈H₁₅NO₂	2.09
	7H-Dibenzo(a,g)carbazole	C₂₀H₁₃N	0.64
	6-Benzamido-4-benzoyl-1,2,4-triazine-3,5(2H,4H)-dione	C₁₇H₁₂N₄O₄	0.93
	Thiazole	C₃H₃NS	0.78
	Phenol, 2-amino-5-methyl-	C₇H₉NO	2.02
	Phenylephrine	C₉H₁₃NO₂	1.92
	Phenol, 2-(2-furyl)(2-pyrimidylamino)methyl-	C₁₅H₁₃N₃O₂	0.47
	5-Amino-7-(4-methoxy-phenyl)-2-phenyl-pyrazolo[1,5-a]pyrimidine-6-carbonitrile	C₂₀H₁₅N₅O	0.50
	1H-Imidazole-1-ethanol,-methyl-	C₆H₁₀N₂O	3.88
	2,5-Pyridinedicarboxylic acid	C₇H₅NO₄	0.98
	4,6-Diamino-O-cresol	C₇H₁₀N₂O	0.43
	1,2,3-Benzothiadiazol-5-ol	C₆H₄N₂OS	0.49
	2,4-Dimethoxy-5-pyrimidine carboxaldehyde	C₇H₈N₂O₃	1.20
	9H-Pyrido[3,4-b]indole	C₁₁H₈N₂	1.62
	Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-	C₇H₁₀N₂O₂	5.20
	2,4-Dihydroxypyridine	C₅H₅NO₂	1.58
	Pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl)-	C₁₁H₁₈N₂O₂	0.68
	4,4-Dimethyl-2,5-dioxo-1-vinylimidazolidine	C₇H₁₀N₂O₂	0.86
	Chlorpropham	C₁₀H₁₂ClNO₂	0.69
	Cyclohexanecarbonitrile, 4-oxo-1-phenyl-	C₁₃H₁₃NO	0.51
	1,3-Benzodioxole-5-carboxylic acid, 6-acetamino-	C₁₀H₉NO₅	0.69
	Butyric acid, 4-(2-carbamoylpyrrolidin-1-yl)-4-oxo-	C₉H₁₄N₂O₄	1.05
	3,6-Diisopropyl-2,5-dioxomorpholine	C₁₀H₁₇NO₃	0.77
	4-Aminoresorcinol	C₆H₇NO₂	0.72
	Isoproterenol tri-TMS derivative	C₁₁H₁₇NO₃	0.66
	Thiophene-2-carboxylic acid, 4,5-dimethyl-3-(1-pyrrolyl)-	C₁₁H₁₁NO₂S	2.52
	Ergosta-7,22-dien-6-one, 3-hydroxy-, O-methyloxime	C₂₉H₄₇NO₂	1.94
	Indazol-4-one, 5,6-dimethyl-2-phenyl-3-pyridin-3-yl -2,5,6,7-tetrahydro-	C₂₀H₁₉N₃O	1.56
	1H-Carbazole-9-ethanol, 2,3,4,9-tetrahydro-[[(1-methyl-1H-imidazol-2-yl)thio]methyl]-	C₁₉H₂₃N₃OS	1.24
	1-Benzo[1,3]dioxol-5-ylmethyl-6,7-dimethoxy-2-nitroso-1,2,3,4-tetrahydroisoquinolin-4-ol	C₁₉H₂₀N₂O₆	0.42
	3-Methyl-butyric acid, 4-butyl-2-methyl-5-oxotetrahydrofuran-3-yl ester	C₁₃H₁₉NO₃	0.57
	Carbamic acid, methyl-, phenyl ester	C₈H₉NO₂	0.68
	Furo[3,4-c]pyridin-1(3H)-one, 7-hydroxy-6-methyl-	C₈H₇NO₃	0.55
	Acetic acid, cyano-, 2-methoxyethyl ester	C₆H₉NO₃	0.87
	小计		59.10
香草醛或香豆素类化合物	Vanillin	C₈H₈O₃	1.71
	Benzaldehyde, 3,4-dihydroxy-5-methoxy-	C₈H₈O₄	0.91
	Benzaldehyde, 2,5-dimethoxy-	C₉H₁₀O₃	1.33
	Benzaldehyde, 2-hydroxy-3-methoxy-	C₈H₈O₃	1.43
	8-Hydroxycoumarin	C₉H₆O₃	1.25
	3-n-Butyrylcoumarin	C₁₃H₁₂O₃	0.56
	小计		7.19
羧酸及酯类化合物	4-Acetylbutyric acid	C₆H₁₀O₃	1.60
	4-Acetylbenzoic acid	C₉H₈O₃	0.89
	Crotonic acid	C₄H₆O₂	2.96
	4-Acetoxycinnamic acid	C₁₁H₁₀O₄	1.47
	3-Phenylpropionic acid, 5-methoxy-2-[3,4-dimethoxyphenyl]-	C₁₈H₂₀O₅	1.63
	Propionylfilicinic acid	C₁₁H₁₄O₄	0.86
	3-(3-Hydroxyphenyl)propionic acid	C₉H₁₀O₃	1.01
	Protocatechuic acid	C₇H₆O₄	3.09
	3,5-di-tert-Butyl-4-hydroxyphenylpropionic acid	C₁₇H₂₆O₃	0.63
	Dodecanoic acid, 2-methyl-	C₁₃H₂₆O₂	0.51
	Methyl 4-methoxysalicylate	C₉H₁₀O₄	0.55
	Benzoic acid, 2-hydroxy-, pentyl ester	C₁₂H₁₆O₃	0.88
	Benzoic acid, 2,3,4-trihydroxy-, methyl ester	C₈H₈O₅	1.12
	1-Butanol, 3-methyl-, acetate	C₇H₁₄O₂	0.77
	1,5-Hexadiene-3-carboxylic acid, 1-methoxy-5-methyl-, methyl ester	C₁₀H₁₆O₃	0.59
	Octadecanoic acid, methyl ester	C₁₉H₃₈O₂	0.70
	小计		19.26
酮类化合物	1-Indanone, 5,6-dimethyl-	C₁₁H₁₂O	0.72
	2,6-Dihydroxyacetophenone	C₈H₈O₃	0.93
	2,5-Dihydroxypropiophenone	C₉H₁₀O₃	1.38
	2-Pentanone, 4-hydroxy-4-methyl-	C₆H₁₂O₂	1.00
	7,7-Bis-hydroxymethyl-1,4-dioxaspiro[4.5]decan-8-one	C₁₀H₁₆O₅	0.83
	小计		4.86
醇（酚）类化合物	3,3-Dimethyl-2-pentanol	C₇H₁₆O	0.60
	1,2-Propanediol, 3-methoxy-	C₄H₁₀O₃	1.14
	2-Propylcyclohexanol	C₉H₁₈O	0.46
	(2,3-Dimethyloxiranyl) methanol	C₅H₁₀O₂	1.28
	Vinylfuran	C₆H₆O	0.93
	1-(2-Furyl)-3-buten-1-ol	C₈H₁₀O₂	0.57
	1,4-Benzenediol, 2,3,5-trimethyl-	C₉H₁₂O₂	0.93
	trans-4-Methyl-5-methoxy-1-(1-hydroxy-1-isopropyl)cyclohex-3-ene	C₁₁H₂₀O₂	0.47
	2(R),3(S)-1,2,3,4-Butanetetrol	C₄H₁₀O₄	1.05
	小计		7.43
Citral		C₁₀H₁₆O	1.57
3H-Benz(e)indene, 2-methyl-		C₁₄H₁₂	0.60

化合物	浓度/(μmol/L)	抑制率/%
化合物	浓度/(μmol/L)	株高	根长
胆酸	1000	14.14±3.05a	20.51±1.86a
	500	-3.95±0.07b	9.56±1.08b
	250	-8.64±0.18b	-19.56±6.10c
邻香豆酸	1000	36.70±3.06a	77.71±6.02a
	500	21.46±5.45b	75.72±1.03a
	250	21.05±1.06b	70.90±6.02ab
反式-2,4-癸二烯醛	1000	31.53±2.34a	6.70±1.20a
	500	-6.04±0.46b	2.57±0.10ab
	250	-28.81±1.47c	-3.20±0.15c
α-蒎烯	1000	-42.91±5.07a	-24.69±1.11b
	500	-40.01±3.16a	-10.17±1.09a
	250	-32.40±4.46b	-8.31±0.09a
亚油酸	1000	31.88±2.04a	23.24±1.08a
	500	12.25±2.16b	-16.35±1.14b
	250	-8.91±1.07c	-21.75±1.11c
脯氨酸	1000	-28.59±3.17a	2.08±0.08a
	500	-30.02±1.35b	-15.95±1.08b
	100	-47.62±4.04c	-22.62±3.10c
酪氨酸	1000	-59.30±2.36c	-36.75±1.14c
	500	-29.62±1.35b	17.49±0.27b
	100	-18.76±1.36a	52.04±4.06a
香豆素	1000	-13.56±1.04c	-33.40±3.10c
	500	26.08±1.32b	66.85±4.02ab
	100	39.05±1.01a	73.35±3.82a
柠檬醛	150	85.82±3.23a	100.00 a
	110	54.16±3.14b	25.37±2.06b
	100	6.33±1.11c	5.52±0.31c
香兰素	1000	19.93±1.23a	72.58±0.15a
	500	5.95±0.13b	41.13±7.32b
	100	-6.12±0.43c	-42.28±2.66c
胡椒碱	125	17.39±1.04a	76.30±2..12a
	75	10.36±1.34b	75.93±3.04a
	25	3.24±0.56c	75.98±4.02a

一株抑草真菌发酵液中的抑草成分分析

Herbicide Ingredients in Fermentation Broth of One Strain of Fungi: Analysis

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