不同产地黄芩主要化学成分含量及抗肿瘤活性比较研究

doi:10.11924/j.issn.1000-6850.casb2021-0248

摘要/Abstract

摘要：

本研究旨在探究不同产地对黄芩的化学成分含量及抗肿瘤活性的影响。通过HPLC色谱法测定黄芩中黄芩苷、汉黄芩苷、黄芩素、汉黄芩素的含量差异,采用CCK-8法检测不同产地的黄芩对抑制A549肺癌细胞增殖的情况。结果表明,不同产地黄芩样品中的化学成分含量有显著差异,黄芩苷含量由高到低为黑龙江勃利>山东莒县>甘肃定西>山西运城,汉黄芩苷含量由高到低为黑龙江勃利>甘肃定西>山东莒县>山西运城,黄芩素含量由高到低为黑龙江勃利>山东莒县>山西运城>甘肃定西,汉黄芩素含量由高到低为黑龙江勃利>山西运城>山东莒县>甘肃定西。综上,黑龙江勃利地区黄芩中的化学成分含量高居于4个产地首位。黄芩提取物对A549肺癌细胞有抑制作用,且抑制作用与其化学成分含量有关,并呈现剂量依赖。实验结果可为寒地黄芩的开发及合理利用药用资源提供一些参考。

关键词: 黄芩, HPLC色谱法, 黄芩苷, 汉黄芩苷, 黄芩素, 汉黄芩素, 抗肿瘤活性

Abstract:

The purpose of the study is to investigate the effect of different origins on the contents of the chemical components and anti-tumor activity Scutellaria baicalensis. The study used HPLC chromatography to determine the contents of baicalin, wogonoside, baicalein and wogonin of S. baicalensis from different origins, and CCK-8 method to detect the inhibition of A549 lung cancer cell proliferation. The results show that there are significant differences in the contents of chemical components of S. baicalensis samples from different origins. The content of baicalin from high to low is Boli of Heilongjiang> Juxian of Shandong> Dingxi of Gansu> Yuncheng of Shanxi; the content of wogonoside from high to low is Boli of Heilongjiang> Dingxi of Gansu> Juxian of Shandong> Yuncheng of Shanxi; the content of baicalein from high to low is Boli of Heilongjiang> Juxian of Shandong> Yuncheng of Shanxi> Dingxi of Gansu; and the wogonin content from high to low is Boli of Heilongjiang> Yuncheng of Shanxi> Juxian of Shandong> Dingxi of Gansu. As shown above, the contents of the chemical components of S. baicalensis in Boli of Heilongjiang rank the first among those from the 4 origins. The extract of S. baicalensis has an inhibitory effect on A549 lung cancer cells, and the effect is related to the contents of the chemical components and dose-dependent. The experimental results can provide reference for the development and rational use of medicinal resources of S. baicalensis.

Key words: Scutellaria baicalensis, HPLC chromatography, baicalin, wogonoside, baicalein, wogonin, anti-tumor activity

中图分类号:

S182

房佳敏, 曾伟民, 张彦龙. 不同产地黄芩主要化学成分含量及抗肿瘤活性比较研究[J]. 中国农学通报, 2022, 38(4): 120-126.

FANG Jiamin, ZENG Weimin, ZHANG Yanlong. The Contents and Anti-tumor Activity of Main Chemical Components of Scutellaria baicalensis from Different Origins: A Comparative Study[J]. Chinese Agricultural Science Bulletin, 2022, 38(4): 120-126.

图/表 12

参考文献 25

[1]	王津燕. 中药黄芩药理作用的研究进展[J]. 内蒙古中医药, 2020, 39(2):167-168.
[2]	国家卫生健康委办公厅, 国家中医药管理办公室. 关于印发新型冠状病毒感染的肺炎诊疗方案(试行第八版)的通知[Z]. 2020.
[3]	杨志华, 闫海峰, 闫艳芳, 等. 基于网络药理学探讨双黄连口服液抑制新型冠状病毒(2019-nCoV)的分子机制研究[J]. 中药材, 2020(9):2333-2340.
[4]	LU Gejin, XU Lei, ZHANG Tong, et al. A potential bio-control agent from baical skullcap root against listeriosis via the inhibition of sortase A and listeriolysin O[J]. Journal of cellular and molecular medicine, 2019, 23(3).
[5]	王文涛, 陈瑞, 曹瑶, 等. 中药黄芩资源研究进展[J]. 陕西农业科学, 2019, 65(4):87-91.
[6]	BAI Chengke, YANG Jingjing, CAO Bo, et al. Growth years and post-harvest processing methods have critical roles on the contents of medicinal active ingredients of Scutellaria baicalensis[J]. Industrial crops and products, 2020, 158.
[7]	SONG Lijun, ZHONG Peicheng, ZHU Xuemei, et al. The anti-rotavirus effect of baicalin via the gluconeogenesis-related p-JNK-PDK1-AKT-SIK2 signaling pathway[J]. European journal of pharmacology, 2021, 897.
[8]	QIAO Xue, LI Ru, SONG Wei, et al. A targeted strategy to analyze untargeted mass spectral data: Rapid chemical profiling of Scutellaria baicalensis using ultra-high performance liquid chromatography coupled with hybrid quadrupole orbitrap mass spectrometry and key ion filtering[J]. Journal of chromatography A, 2016, 1441.
[9]	YU Xiu, CHEN Dandan, WANG Lingwei, et al. Wogonoside inhibits inflammatory cytokine production in lipopolysaccharide-stimulated macrophage by suppressing the activation of the JNK/c-Jun signaling pathway[J]. Annals of translational medicine, 2020, 8(8).
[10]	HUYNH Do Luong, NGAU Tran Hoang, NGUYEN Nguyen Hoai, et al. Potential therapeutic and pharmacological effects of Wogonin: An updated review[J]. Molecular biology reports, 2020, 47(12).
[11]	刘梦珂, 纪濛濛, 程林, 等. 黄芩苷抗肿瘤作用机制的研究进展[J]. 上海交通大学学报:医学版, 2021, 41(2):1674-8115.
[12]	JIANG Guangyu, CHEN Dayin, LI Wenpeng, et al. Effects of wogonoside on the inflammatory response and oxidative stress in mice with nonalcoholic fatty liver disease[J]. Pharmaceutical biology, 2020, 58(1).
[13]	WANG Chaoyue, ZUO Guanglei, WANG Xiang, et al. Retention mechanism of pH-peak-focusing in countercurrent chromatographic separation of baicalin and wogonoside from Scutellaria baicalensis Georgi[J]. Journal of separation science, 2020, 43(19).
[14]	刘青青, 王玉波, 刘建伟, 等. 同时测定黄芩提取液中黄芩苷、汉黄芩苷、黄芩素含量的高效液相色谱方法的建立[J]. 滨州医学院学报, 2020, 43(1):54-57.
[15]	CHENG Yingqiu, LI Shipeng, LIU Yi, et al. Treatment of brain edema by wogonoside is associated with inhibition of neuronal apoptosis and SIRT1 activation in rats[J]. Medical science monitor, 2020, 26.
[16]	HUANG Yujie, FANG Jiansong, LU Weiqiang, et al. A systems pharmacology approach uncovers wogonoside as an angiogenesis inhibitor of triple-negative breast cancer by targeting hedgehog signaling[J]. Cell chemical biology, 2019, 26(8).
[17]	廉政君, 常炜. 汉黄芩素调节JAK/STAT信号通路并诱导肺癌A549细胞凋亡及周期阻滞的作用及机制研究[J]. 临床肺科杂志, 2021, 26(2):270-274,279.
[18]	JI Yeongseon, HAN Jin, LEE Nayoung, et al. Neuroprotective effects of baicalein, wogonin, and oroxylin A on amyloid beta-Induced toxicity via NF-κB/MAPK pathway modulation[J]. Molecules, 2020, 25(21).
[19]	白荣, 白洪越, 孔水仙, 等. 汉黄芩素和汉黄芩苷对UGT1A1体外抑制活性的比较研究[J]. 中国中医药科技, 2020, 27(5):707-711.
[20]	杨洋, 王志强, 任丽丽, 等. 不同产地黄芩药材中主要药效成分含量变化规律研究[J]. 天津中医药大学学报, 2020, 39(3):324-329.
[21]	王林, 吴翠芸, 汪贤竹, 等. 黄芩苷对肺癌A549细胞自噬及上皮间质转化的影响[J]. 中国细胞生物学学报, 2020, 42(11):1960-1968.
[22]	CAO Xuexiao, YOU Guangjiao, LI Huanhuan, et al. Comparative investigation for rotten xylem (kuqin) and strip types (tiaoqin) of Scutellaria baicalensis Georgi based on fingerprinting and chemical pattern recognition[J]. Molecules, 2019, 24(13).
[23]	田甜. 不同产地和生长年限黄芩质量评价及药理作用研究[D]. 杭州:浙江理工大学, 2018.
[24]	王常瞵, 高鹏, 姜晶晶, 等. 中药黄芩指纹图谱研究进展[J]. 辽宁中医药大学学报, 2020, 22(4):82-86.
[25]	中华人民共和国药典委员会. 中国药典一部[M]. 北京: 化学工业出版社, 2015:562-563.

样品编号	产地	生长方式	生长年限/年
S1	黑龙江勃利	栽培	2
S2	山东莒县	栽培	2
S3	山西运城	栽培	2
S4	甘肃定西	栽培	2

样品编号	产地	生长方式	生长年限/年
S1	黑龙江勃利	栽培	2
S2	山东莒县	栽培	2
S3	山西运城	栽培	2
S4	甘肃定西	栽培	2

时间/min	乙腈浓度/%	时间/min	乙腈浓度/%
0~10	8→12	70~73	63→63
10~20	12→17	73~77	63→70
20~30	17→24	77~80	70→80
30~60	24→34	80~85	80→8
60~70	34→63	85~90	8

时间/min	乙腈浓度/%	时间/min	乙腈浓度/%
0~10	8→12	70~73	63→63
10~20	12→17	73~77	63→70
20~30	17→24	77~80	70→80
30~60	24→34	80~85	80→8
60~70	34→63	85~90	8

溶剂名称	黄芩苷峰面积	汉黄芩苷峰面积	黄芩素峰面积	汉黄芩素峰面积
70%乙醇	50445983	10892585	5182270	1055412
80%甲醇	59529640	11976181	5919874	1111190
60%丙酮	36586637	8250972	3443369	1012837