不同方法提取的生物质炭可溶性有机物性质研究

doi:10.11924/j.issn.1000-6850.casb2022-0434

中国农学通报 ›› 2023, Vol. 39 ›› Issue (12): 61-68.doi: 10.11924/j.issn.1000-6850.casb2022-0434

• 资源·环境·生态·土壤 • 上一篇下一篇

不同方法提取的生物质炭可溶性有机物性质研究

郑小东(), 李翔, 魏岚, 黄连喜, 陈伟盛, 黄玉芬, 黄庆, 刘忠珍()

广东省农业科学院农业资源与环境研究所，农业农村部南方植物营养与肥料重点实验室，广东省养分资源循环利用与耕地保育重点实验室，广州 510640

收稿日期:2022-05-31 修回日期:2022-08-15 出版日期:2023-04-25 发布日期:2023-04-21
通讯作者: 刘忠珍，女，1977年出生，河南新乡人，研究员，博士，研究方向：土壤污染物治理。通信地址：510640 广州天河区金颖路66号广东省农业科学院农业资源与环境研究所，Tel：020-38085151，E-mail：lzzgz2001@163.com。
作者简介:
郑小东，男，1987年出生，博士，研究方向：环境化学。通信地址：510640 广州天河区金颖路66号广东省农业科学院农业资源与环境研究所，Tel：020-85161402，E-mail：zxdmrhq@foxmail.com。
基金资助:
广东省农业科学院低碳农业与碳中和研究中心“基于农业废弃物还田利用对土壤碳转化及固碳效应研究”(XTXM202204); 国家自然科学基金项目“生物炭调控可溶性有机物微生物转化过程及机理”(42207316); 广东省农科院新兴团队项目“土壤质量与污染控制”(202120TD); 广东省科技计划项目“广东省稻田不同耕作模式碳足迹及固碳效应野外科学观测研究站”(2021B1212050020); 2022年省级涉农统筹整合转移支付资金“2022年江门市本级推广耕地质量提升技术-菜地化肥减施增效与有机肥替代技术试验示范”(江财农[2021]126号)

Biochar-derived Dissolved Organic Matter Extracted by Different Methods: Property Study

ZHENG Xiaodong(), LI Xiang, WEI Lan, HUANG Lianxi, CHEN Weisheng, HUANG Yufen, HUANG Qing, LIU Zhongzhen()

Key Laboratory of Plant Nutrition and Fertilizer in South Region, Ministry of Agriculture and Rural Affairs;Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation;Institute of Agricultural Resources and Environment, Guangdong Academy of Agricultural Sciences, Guangzhou 510640

Received:2022-05-31 Revised:2022-08-15 Online:2023-04-25 Published:2023-04-21

摘要/Abstract

摘要：

为了揭示不同提取方法对生物质炭来源可溶性有机物(DOM)性质的影响，以生物质炭为研究对象，采用多种提取剂、提取方式提取生物质炭DOM，分析其碳含量及化学组成特征。结果表明：低温生物质炭中碱提取物DOC含量较高(15.6~40.0 g/kg)，而高温生物质炭中盐提取物较高(0.27~7.04 g/kg)。酸提取物DOM化学组成较为简单，表现为SUVA₂₅₄、SUVA₂₈₀值较低，且玉米秆生物质炭中酸提取物亲水性DOM比例(44.6%~73.6%)显著高于水和碱提取物(11.0%~53.2%、0.30%~31.4%)。碱提取物DOM化学组成较复杂，其SUVA₂₅₄、SUVA₂₈₀值较高，同时玉米秆生物质炭中碱提取物疏水性DOM比例(68.6%~99.7%)显著高于酸和盐提取物(26.4%~55.4%、0%~46.9%)。该研究揭示了提取剂在生物质炭DOM提取方法中的重要性，而提取方式对其性质影响不显著，可为生物质炭DOM提取方法的选择提供参考。

关键词: 生物质炭, 可溶性有机物, 提取方法, 化学组成, 土壤重金属污染修复

Abstract:

This study aims at investigating the impact of different extraction methods on the properties of dissolved organic matter (DOM) derived from biochar. Biochar was used as material, DOM was extracted with various extracting agents and extraction patterns, and the carbon content and chemical composition of the extracts were analyzed. The results showed that the DOC content of alkali extract in low-temperature biochar was relatively high (15.6-40.0 g/kg), so was the DOC content of salt extract in high-temperature biochar (0.27-7.04 g/kg). The chemical composition of DOM in the acid extract remained relatively simple, showing that SUVA₂₅₄ and SUVA₂₈₀ were low. A higher proportion of hydrophilic DOM was found in the acid extract from cornstalk-derived biochar (44.6%-73.6%) compared with that in the water and alkali extracts (11.0%-53.2% and 0.30%-31.4%), respectively (P<0.05). Correspondingly, the chemical composition of DOM in the alkali extract was relatively complex, showing that SUVA₂₅₄ and SUVA₂₈₀ were high. A higher proportion of hydrophobic DOM was found in the alkali extract from cornstalk-derived biochar (68.6%-99.7%) compared with that in the acid and salt extracts (26.4%-55.4% and 0%-46.9%), respectively (P<0.05). The study indicates that extracting agents have certain significance in biochar DOM extraction, while extraction patterns exhibit a minor effect on the properties of biochar DOM. It could provide reference for selecting biochar DOM extraction method.

Key words: biochar, dissolved organic matter, extraction method, chemical composition, soil heavy metal contamination remediation

郑小东, 李翔, 魏岚, 黄连喜, 陈伟盛, 黄玉芬, 黄庆, 刘忠珍. 不同方法提取的生物质炭可溶性有机物性质研究[J]. 中国农学通报, 2023, 39(12): 61-68.

ZHENG Xiaodong, LI Xiang, WEI Lan, HUANG Lianxi, CHEN Weisheng, HUANG Yufen, HUANG Qing, LIU Zhongzhen. Biochar-derived Dissolved Organic Matter Extracted by Different Methods: Property Study[J]. Chinese Agricultural Science Bulletin, 2023, 39(12): 61-68.

图/表 6

生物质炭类型	表面积/(m²/g)	孔直径/nm	N/%	C/%	H/%	H/C	O/C
玉米秆-低温	3.33	1.36	2.75	53.7	3.70	0.07	0.74
玉米秆-高温	3.05	4.72	1.59	65.2	2.02	0.03	0.48
猪粪-低温	16.2	1.18	2.23	19.4	1.75	0.09	3.92
猪粪-高温	52.5	1.19	0.33	9.59	0.92	0.10	9.20

表1. 生物质炭基本性质

图1. 不同提取剂及提取方式下生物质炭DOC含量特征图中不同字母表示处理间在P<0.05水平差异显著

图2. 不同提取剂及提取方式下生物质炭DOM吸光度指标特征图中不同字母表示处理间在P<0.05水平差异显著

Hi-DOM组分/%
生物质炭原料	编号	水	酸	碱	盐
玉米秆	1	53.2	73.6	31.4	46.6
	2	25.4	44.6	0.3	82.4
	3	35.1	60.5	17.3	57.5
	4	11	63.2	20.2	100
猪粪	1	57.1	53.4	80.8	67.2
	2	90.6	62	93.8	100
	3	81.1	78.7	75.4	87.4
	4	100	81	100	89
玉米秆	1	46.7	26.4	68.6	46.9
	2	74.6	55.4	99.7	17.6
	3	64.9	38.7	82.7	42.5
	4	89	36.8	79.8	0
猪粪	1	42.9	46.6	19.2	32.8
	2	9.4	38	6.2	0
	3	18.9	21.3	24.6	12.6
	4	0	19	0	11

表2. 不同提取剂及提取方法下生物质炭Hi-DOM和Ho-DOM组分比例

图3. 不同提取剂及提取方式下生物质炭DOM HIX指数特征图中不同字母表示处理间在P<0.05水平差异显著

图4. 基于随机森林方法的生物质炭DOM各指标影响因素分析图中表示处理间在P<0.05水平差异显著，表示处理间在P<0.01水平差异显著

参考文献 39

[1]	陈温福, 张伟明, 孟军. 农用生物炭研究进展与前景[J]. 中国农业科学, 2013, 46(16):3324-3333. doi: 10.3864/j.issn.0578-1752.2013.16.003
[2]	廖聪坚, 赵晓蕾, 刘凯, 等. 电活性微生物激活生物质炭/零价铁协同钝化Cr(Ⅵ)及机制[J]. 环境科学, 2021, 42(9):4520-4526.
[3]	卞荣军, 刘晓雨, 郑聚锋, 等. 生物质炭可溶性有机物化学组成及生物活性意义[J]. 中国农业科学, 2022, 55(11):2174-2186. doi: 10.3864/j.issn.0578-1752.2022.11.008
[4]	BORGGAARD O K, HOLM P E, STROBEL B W, et al. Potential of dissolved organic matter (DOM) to extract As, Cd, Co, Cr, Cu, Ni, Pb and Zn from polluted soils: a review[J]. Geoderma, 2019, 343:235-246. doi: 10.1016/j.geoderma.2019.02.041 URL
[5]	ZHAO C, GAO S J, ZHOU L, et al. Dissolved organic matter in urban forestland soil and its interactions with typical heavy metals: a case of Daxing District, Beijing[J]. Environmental science and pollution research, 2019, 26:2960-2973. doi: 10.1007/s11356-018-3860-7
[6]	YUAN D H, AN Y C, HE X S, et al. Fluorescent characteristic and compositional change of dissolved organic matter and its effect on heavy metal distribution in composting leachates[J]. Environmental science and pollution research, 2018, 25:18866-18878. doi: 10.1007/s11356-018-2067-2
[7]	HUANG M H, LI Z W, HUANG B, et al. Investigating binding characteristics of cadmium and copper to DOM derived from compost and rice straw using EEM-PARAFAC combined with two-dimensional FTIR correlation analyses[J]. Journal of hazardous materials, 2018, 344:539-548. doi: S0304-3894(17)30771-9 pmid: 29101883
[8]	SMITH C R, HATCHER P G, KUMAR S, et al. Investigation into the sources of biochar water-soluble organic compounds and their potential toxicity on aquatic microorganisms[J]. ACS sustainable chemistry and engineering, 2016, 4:2550-2558. doi: 10.1021/acssuschemeng.5b01687 URL
[9]	FU H Y, LIU H T, MAO J D, et al. Photochemistry of dissolved black carbon released from biochar: reactive oxygen species generation and phototransformation[J]. Environmental science and technology, 2016, 50:1218-1226. doi: 10.1021/acs.est.5b04314 pmid: 26717492
[10]	WEI J, TU C, YUAN G D, et al. Limited Cu (II) binding to biochar DOM: Evidence from C K-edge NEXAFS and EEM-PARAFAC combined with two-dimensional correlation analysis[J]. Science of the total environment, 2020, 701:1-10.
[11]	韩成卫, 李忠佩, 刘丽, 等. 去除溶解性有机质对红壤水稻土碳氮矿化的影响[J]. 中国农业科学, 2007, 40(1):107-113.
[12]	李玲, 肖和艾, 苏以荣, 等. 土地利用对亚热带红壤区典型景观单元土壤溶解有机碳含量的影响[J]. 中国农业科学, 2008, 41(1): 122-128.
[13]	ZHENG X D, CHEN X B, LIANG C M, et al. Influence of extractants and filter materials in the extraction of dissolved organic matter (DOM) from subtropical agricultural soil[J]. Emirates journal of food and agriculture, 2018, 30(3):165-172.
[14]	ENDERS A, HANLEY K, WHITMAN T, et al. Characterization of biochars to evaluate recalcitrance and agronomic performance[J]. Bioresource technology, 2012, 114:644-653. doi: 10.1016/j.biortech.2012.03.022 pmid: 22483559
[15]	袁帅, 赵立欣, 孟海波, 等. 生物炭主要类型、理化性质及其研究展望[J]. 植物营养与肥料学报, 2016, 22(5):1402-1417.
[16]	RAJAPAKSHA A U, OK Y S, NAGGAR A E, et al. Dissolved organic matter characterization of biochars produced from different feedstock materials[J]. Journal of environmental management, 2019, 233:393-399. doi: S0301-4797(18)31491-9 pmid: 30590268
[17]	高洁, 江韬, 李璐璐, 等. 三峡库区消落带土壤中可溶性有机质(DOM)吸收及荧光光谱特征[J]. 环境科学, 2015, 36(1):151-162.
[18]	郭晓慧, 康康, 于秀男, 等. 磁改性柚子皮与杏仁壳生物炭的理化性质研究[J]. 农业工程学报, 2018, 34(S1):164-171.
[19]	LAWAETZ A J, STEDMON C A. Fluorescence intensity calibration using the Raman scatter peak of water[J]. Applied spectroscopy, 2009, 63(8):936-940. doi: 10.1366/000370209788964548 pmid: 19678992
[20]	郑小东, 陈香碧, 胡亚军, 等. 亚热带典型地貌下旱地和水田可溶性有要物化学组成特征[J]. 土壤, 2019, 51(6):1114-1121.
[21]	PLANT E L, SMERNIK R J, LEEUWEN J V, et al. Changes in the nature of dissolved organics during pulp and paper mill wastewater treatment: a multivariate statistical study combining data from three analytical techniques[J]. Environmental science and pollution research, 2014, 21(6):4265-4275. doi: 10.1007/s11356-013-2351-0 URL
[22]	PULLICINO D S, GIGLIOTTI G. Oxidative biodegradation of dissolved organic matter during composting[J]. Chemosphere, 2007, 68:1030-1040. doi: 10.1016/j.chemosphere.2007.02.012 URL
[23]	KALMYKOVA Y, MOONA N, STROMVALL A M, et al. Sorption and degradation of petroleum hydrocarbons, polycyclic aromatic hydrocarbons, alkylphenols, bisphenol A and phthalates in landfill leachate using sand, activated carbon and peat filters[J]. Water resources, 2014, 56:246-257.
[24]	QU X L, FU H Y, MAO J D, et al. Chemical and structural properties of dissolved black carbon released from biochars[J]. Carbon, 2016, 96:759-767. doi: 10.1016/j.carbon.2015.09.106 URL
[25]	CHEN Z, XIAO X, CHEN B, et al. Quantification of chemical states, dissociation constants and contents of oxygen-containing groups on the surface of biochars produced at different temperatures[J]. Environmental science and technology, 2015, 49(1):309-317. doi: 10.1021/es5043468 pmid: 25453912
[26]	JAFFRAIN J, GERARD F, MEYER M, et al. Assessing the quality of dissolved organic matter in forest soils using ultraviolet absorption spectrophotometry[J]. Soil science society of America journal, 2007, 71(6):1851-1858. doi: 10.2136/sssaj2006.0202 URL
[27]	LIU C H, CHU W Y, LI H, et al. Quantification and characterization of dissolved organic carbon from biochars[J]. Geoderma, 2019, 335:161-169. doi: 10.1016/j.geoderma.2018.08.019 URL
[28]	JONES D L, WILLETT B. Experimental evaluation of methods to quantify dissolved organic nitrogen (DON) and dissolved organic carbon (DOC) in soil[J]. Soil biology and biochemistry, 2006, 38:991-999. doi: 10.1016/j.soilbio.2005.08.012 URL
[29]	施暖暖, 李婉秋, Marios Drosos, 等. 不同原料生物质炭DOM组分成分特性[J]. 环境科学学报, 2022, 42(9):281-290.
[30]	LIU P, PTACEK C J, BLOWES D W, et al. Aqueous leaching of organic acids and dissolved organic carbon from various biochars prepared at different temperatures[J]. Journal of environmental quality, 2015, 44:684-695. doi: 10.2134/jeq2014.08.0341 pmid: 26023986
[31]	LIN Y, MUNROE P, JOSEPH S, et al. Water extractable organic carbon in untreated and chemical treated biochars[J]. Chemosphere, 2012, 87:151-157. doi: 10.1016/j.chemosphere.2011.12.007 pmid: 22236590
[32]	WU H M, QI Y S, DONG L, et al. Revealing the impact of pyrolysis temperature on dissolved organic matter released from the biochar prepared from Typha orientalis[J]. Chemosphere, 2019, 228:264-270. doi: S0045-6535(19)30797-0 pmid: 31035164
[33]	WEI J, TU C, YUAN G D, et al. Assessing the effect of pyrolysis temperature on the molecular properties and copper sorption capacity of a halophyte biochar[J]. Environmental pollution, 2019, 251:56-65. doi: S0269-7491(18)34847-4 pmid: 31071633
[34]	WEI J, TU C, YUAN G D, et al. Pyrolysis temperature-dependent changes in the characteristics of biochar-borne dissolved organic matter and its copper binding properties[J]. Bulletin of environmental contamination and toxicology, 2019, 103:169-174. doi: 10.1007/s00128-018-2392-7 pmid: 29982867
[35]	朱金霞, 孔德杰, 尹志荣. 农作物秸秆主要化学组成及还田后对土壤质量提升影响的研究进展[J]. 北方园艺, 2020, 5:146-153.
[36]	王立华, 林琦. 热解温度对畜禽粪便制备的生物质炭性质的影响[J]. 浙江大学学报, 2014, 41(2):185-190.
[37]	CHEN C M, SPARKS D L. Multi-elemental scanning transmission X-ray microscopy-near edge X-ray absorption fine structure spectroscopy assessment of organo-mineral associations in soils from reduced environments[J]. Environmental chemistry, 2015, 1:64-73.
[38]	STRAATHOF A L, CHINCARINI R, COMANS N, et al. Dynamics of soil dissolved organic carbon pools reveal both hydrophobic and hydrophilic compounds sustain microbial respiration[J]. Soil biology and biochemistry, 2014, 79:109-116. doi: 10.1016/j.soilbio.2014.09.004 URL
[39]	MALERBA A D, KAISER K, TAMBONE F, et al. Hydrophilic and hydrophobic fractions of water-soluble organic matter in digestates obtained from different organic wastes[J]. International biodeterioration & biodegradation, 2014, 94:73-78.

不同方法提取的生物质炭可溶性有机物性质研究

Biochar-derived Dissolved Organic Matter Extracted by Different Methods: Property Study

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 39

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	滕柄钦, 马倩倩, 武均, 段雪娇, 毕冬梅, 蔡立群. 生物质炭添加对生菜Cd积累的影响[J]. 中国农学通报, 2023, 39(6): 90-96.
[2]	洪慈清, 桂芳泽, 陈芳容, 方云, 游雨欣, 关雄, 潘晓鸿. 茶渣制备的生物质炭对重金属镍的吸附研究[J]. 中国农学通报, 2022, 38(9): 109-114.
[3]	王海候, 程月琴, 金梅娟, 刘泽凯, 施林林, 陆长婴. 稻壳生物质炭对羊粪堆肥中氮素转化及固定的影响[J]. 中国农学通报, 2022, 38(27): 51-59.
[4]	刘明, 唐佳文, 沙爽, 孟剑侠, 程丽, 李冲伟. 基于分子蒸馏技术紫苏油α-亚麻酸纯化效果的研究[J]. 中国农学通报, 2021, 37(33): 135-141.
[5]	王曼宇, 刘乃新, 张福顺. 植物源性多糖提取及生物活性研究进展[J]. 中国农学通报, 2021, 37(29): 34-41.
[6]	朱锟恒, 段良霞, 李元辰, 李振炜. 土壤团聚体有机碳研究进展[J]. 中国农学通报, 2021, 37(21): 86-90.
[7]	沈芳芳, 张哲, 袁颖红, 周际海. 生物质炭配施有机肥对旱地红壤酶活性及其微生物群落组成的影响[J]. 中国农学通报, 2021, 37(18): 65-74.
[8]	张俊叶, 刘晓东, 王林, 俞元春. 生物质炭的土壤效应研究综述[J]. 中国农学通报, 2020, 36(9): 46-50.
[9]	马涛. 不同裂解温度的污泥生物质炭理化特性分析[J]. 中国农学通报, 2020, 36(35): 134-138.
[10]	李美萍, 董程, 王微, 张生万, 张金才. 辣木籽壳生物炭对溶液中Cu²⁺的吸附性能研究[J]. 中国农学通报, 2020, 36(34): 84-89.
[11]	周加顺, 于洪喜, 杨洁, 孙文青, 高雯雯, 仲雅敏, 史玉洁. 不同用量生物质炭对小白菜和大蒜产量与品质的影响[J]. 中国农学通报, 2020, 36(13): 59-64.
[12]	许唯,胡帅栋,陆鑫华,宋余泽,胡仪. 不同生物质炭对玉米种子萌发及幼苗根系形态特征的影响[J]. 中国农学通报, 2019, 35(29): 20-24.
[13]	李彬豪,游训龙,肖和友,朱伟,邓建功,胡建华. 生物质炭替代草炭对漂浮育苗烟苗生长的影响[J]. 中国农学通报, 2019, 35(17): 32-36.
[14]	应金耀,阮弋飞,邬奇峰,谢国雄,章明奎. 有机肥配施生物质炭对土壤肥力性状与蔬菜生长的影响[J]. 中国农学通报, 2019, 35(16): 82-87.
[15]	余永昌,黄修文,陈秀,乔启成,白晓龙,陈美丽,顾卫兵,徐晨伟,邱建兴,葛成梅. 不同制炭材料与温度对水稻生长发育影响[J]. 中国农学通报, 2019, 35(15): 6-13.