甜菜BvFVE1过表达对抽薹开花影响的研究

doi:10.11924/j.issn.1000-6850.casb2025-0082

中国农学通报 ›› 2026, Vol. 42 ›› Issue (2): 10-15.doi: 10.11924/j.issn.1000-6850.casb2025-0082

甜菜BvFVE1过表达对抽薹开花影响的研究

刘易菲(), 张春雪, 包进梅, 邳植()

黑龙江大学现代农业与生态环境学院，哈尔滨 150080

收稿日期:2025-02-11 修回日期:2025-05-15 出版日期:2026-01-25 发布日期:2026-01-22
通讯作者:
邳植，男，1987年出生，黑龙江哈尔滨人，副研究员，博士，主要从事甜菜遗传及分子育种方面的研究。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院，E-mail：2018060@hlju.edu.cn。
作者简介:
刘易菲，女，2004年出生，广东深圳人，本科，主要从事甜菜遗传及分子育种方面的研究。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院，E-mail：ny0smsy4jh@163.com。
基金资助:
国家自然科学基金“BvFVE1通过调节组蛋白去酰化修饰调控甜菜春化作用的机制研究”(32101765); 大学生创新创业训练计划项目“甜菜HDAC家族成员对抽薹开花相关基因调控作用的初探”(S202310212055)

Effect of BvFVE1 Overexpression on Bolting and Flowering of Sugar Beet

LIU Yifei(), ZHANG Chunxue, BAO Jinmei, PI Zhi()

Academy of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080

Received:2025-02-11 Revised:2025-05-15 Published:2026-01-25 Online:2026-01-22

摘要/Abstract

摘要：

为明确甜菜BvFVE1基因对抽薹开花的调控功能及分子机制，本研究综合采用生物信息学预测、农杆菌介导的瞬时表达、qRT-PCR基因表达分析及拟南芥异源转化技术开展系统研究。首先利用DeepLoc预测和农杆菌瞬时表达分析BvFVE1亚细胞定位。随后，以甜菜KWS9147为试材通过qRT-PCR检测瞬时表达BvFVE1对抽薹开花基因表达的影响。通过浸花法转化拟南芥，观测异源转化BvFVE1对抽薹开花的影响。DeepLoc预测BvFVE1蛋白细胞核和细胞质定位得分分别为0.527和0.4535。农杆菌瞬时表达后可观察到BvFVE1蛋白在叶片下表皮细胞核和细胞质呈点状分布。甜菜子叶瞬时表达BvFVE1后，BvBTC1、BvFT2、BvGI、BvFY表达量分别显著下调2.90、3.27、2.91、2.45倍。BvFVE1异源转化拟南芥后，拟南芥抽薹和开花发生的时间分别延迟约2 d和3 d。这些结果表明BvFVE1对甜菜抽薹开花具有抑制作用。本研究为进一步探索BvFVE1调控甜菜抽薹开花机制提供理论基础。

关键词: 甜菜, BvFVE1, 抽薹, 开花, 春化作用, 亚细胞定位, 瞬时表达, 异源转化

Abstract:

To explore the regulatory role of BvFVE1 in bolting and flowering of sugar beet and to understand the molecular mechanisms of bolting and flowering in sugar beet, the subcellular localization of BvFVE1 was firstly predicted by DeepLoc and analyzed by Agrobacterium-mediated transient expression in this study. Subsequently, sugar beet cultivar KWS9147 was used as the experimental material, and the effect of transient expression of BvFVE1 on the expression of bolting and flowering genes was detected by qRT-PCR. Arabidopsis was transformed via the floral-dip method to observe the effect of heterologous expression of BvFVE1 on bolting and flowering. The DeepLoc prediction showed that the scores for nuclear and cytoplasmic localization of BvFVE1 protein were 0.527 and 0.4535, respectively. After transient expression，BvFVE1 was distributed in a punctate pattern in the nucleus and cytoplasm of leaf epidermal cells. In sugar beet cotyledons, transient expression of BvFVE1 significantly downregulated the expression levels of BvBTC1, BvFT2, BvGI, and BvFY were significantly downregulated by 2.90, 3.27, 2.91, and 2.45 fold, respectively. Heterologous expression of BvFVE1 in Arabidopsis delayed bolting and flowering by approximately 2 and 3 days, respectively. These results indicate that BvFVE1 plays an inhibitory role in bolting and flowering in sugar beet. This study provides a theoretical basis for further investigation into the molecular mechanism by which BvFVE1 regulates bolting and flowering.

Key words: sugar beet, BvFVE1, bolting, flowering, vernalization, subcellular localization, transient expression, heterologous transformation

刘易菲, 张春雪, 包进梅, 邳植. 甜菜BvFVE1过表达对抽薹开花影响的研究[J]. 中国农学通报, 2026, 42(2): 10-15.

LIU Yifei, ZHANG Chunxue, BAO Jinmei, PI Zhi. Effect of BvFVE1 Overexpression on Bolting and Flowering of Sugar Beet[J]. Chinese Agricultural Science Bulletin, 2026, 42(2): 10-15.

图/表 5

图1. BvFVE1表达载体的构建 (A)表达载体图谱；(B)菌液PCR验证；(C)双酶切验证

	细胞质	细胞核	胞外	细胞膜	线粒体	质体	内质网	溶酶体/液泡	高尔基体	过氧化物酶体
阈值	0.4761	0.5014	0.6173	0.5646	0.6220	0.6395	0.6090	0.5848	0.6494	0.7364
预测值	0.4535	0.5257	0.0205	0.1621	0.0708	0.0251	0.1890	0.2153	0.1966	0.0164

表1. DeepLoc预测BvFVE1蛋白亚细胞定位得分

图2. BvFVE1亚细胞定位

图3. 瞬时表达BvFVE1对抽薹开花基因表达的影响 (A)BvFVE1在甜菜子叶中表达情况；(B)抽薹开花相关基因转录表达变化

图4. BvFVE1异源转化拟南芥对抽薹开花的影响 (A)野生型和BvFVE1过表达植株表型；(B)BvFVE1对抽薹开花的影响

参考文献 21

[1]	BLÜMEL M, DALLY N, JUNG C. Flowering time regulation in crops-what did we learn from Arabidopsis?[J]. Current opinion in biotechnology, 2015, 32:121-129. doi: 10.1016/j.copbio.2014.11.023 URL
[2]	HENNIG L, BOUVERET R, GRUISSEM W. MSI1-like proteins: an escort service for chromatin assembly and remodeling complexes[J]. Trends in cell biology, 2005, 15(6):295-302. doi: 10.1016/j.tcb.2005.04.004 pmid: 15953547
[3]	BOUVERET R, SCHÖNROCK N, GRUISSEM W, et al. Regulation of flowering time by Arabidopsis MSI1[J]. Development, 2006, 133(9):1693-1702. doi: 10.1242/dev.02340 URL
[4]	AUSÍN I, ALONSO-BLANCO C, JARILLO J A, et al. Regulation of flowering time by FVE, a retinoblastoma-associated protein[J]. Nature genetics, 2004, 36(2):162-166. doi: 10.1038/ng1295 pmid: 14745447
[5]	XU D, LIU Q, CHEN G, et al. Aldehyde dehydrogenase ALDH3F1 involvement in flowering time regulation through histone acetylation modulation on FLOWERING LOCUS C[J]. Journal of integrative plant biology, 2020, 62(8):1080-1092. doi: 10.1111/jipb.v62.8 URL
[6]	BIANCARDI E. Objectives of sugar beet, Breeding genetics and breeding of sugar beet[M]. Enfield: Science Publishers, Inc, 2005:62-67.
[7]	PI Z, XING W, ZHU X, et al. Temporal expression pattern of bolting-related genes during vernalization in sugar beet[J]. Sugar technology, 2021, 23:146-157. doi: 10.1007/s12355-020-00886-z
[8]	ABOU-ELWAFA S F, BÜTTNER B, CHIA T, et al. Conservation and divergence of autonomous pathway genes in the flowering regulatory network of Beta vulgaris[J]. Journal of experimental botany, 2011, 62(10):3359-3374. doi: 10.1093/jxb/erq321 URL
[9]	DOHM J C, ANDRÉ E M, HOLTGRÄWE D, et al. The genome of the recently domesticated crop plant sugar beet (Beta vulgaris)[J]. Nature, 2014, 505: 546-549. doi: 10.1038/nature12817
[10]	THUMULURI V, ALMAGRO ARMENTEROS J J, JOHANSEN A R, et al. DeepLoc 2.0: multi-label subcellular localization prediction using protein language models[J]. Nucleic acids research, 2022, 50(W1):W228-W234. doi: 10.1093/nar/gkac278 pmid: 35489069
[11]	李君, 李岩, 刘德虎. 植物遗传转化的替代方法及研究进展[J]. 生物技术通报, 2011(7):31-36,45.
[12]	徐蕊, 邳植, 柯立勋, 等. 利用酵母双杂交系统筛选甜菜BvFVE1互作蛋白[J]. 中国糖料, 2025, 47(1):6-14.
[13]	XU Y F, GAN E, ZHOU J, et al. Arabidopsis MRG domain proteins bridge two histone modifications to elevate expression of flowering genes[J]. Nucleic acids research, 2014, 17:10960-10974.
[14]	HELLIWELL C A, WOOD C C, ROBERTSON M, et al. The Arabidopsis FLC protein interacts directly in vivo with SOC1 and FT chromatin and is part of a high-molecular-weight protein complex[J]. The plant journal, 2006, 46(2):183-192. doi: 10.1111/tpj.2006.46.issue-2 URL
[15]	REEVES P A, HE Y, SCHMITZ R J, et al. Evolutionary conservation of the FLOWERING LOCUS C-Mediated vernalization response: Evidence from the sugar beet (Beta vulgaris)[J]. Genetics, 2007, 176(1):295-307. pmid: 17179080
[16]	LUO M, TAI R, YU C W, et al. Regulation of flowering time by the histone deacetylase HDA5 in Arabidopsis[J]. The plant journal, 2015, 82(6):925-936. doi: 10.1111/tpj.2015.82.issue-6 URL
[17]	CHOWDHURY Z, MOHANTY D, GIRI M K, et al. Dehydroabietinal promotes flowering time and plant defense in Arabidopsis via the autonomous pathway genes FLOWERING LOCUS D, FVE, and RELATIVE OF EARLY FLOWERING 6[J]. Journal of experimental botany, 2020, 71(16):4903-4913. doi: 10.1093/jxb/eraa232 URL
[18]	PIN P A, ZHANG W, VOGT S H, et al. The role of a pseudo-response regulator gene in life cycle adaptation and domestication of beet[J]. Current biology, 2012, 22(12):1095-1101. doi: 10.1016/j.cub.2012.04.007 pmid: 22608508
[19]	PIN P A, BENLLOCH R, BONNET D, et al. An antagonistic pair of FT homologs mediates the control of flowering time in sugar beet[J]. Science, 2010, 330:1397-1400. doi: 10.1126/science.1197004 pmid: 21127254
[20]	ZHANG C X, LI S N, WANG Y G, et al. Vernalization promotes bolting in sugar beet by inhibiting the transcriptional repressors of BvGI[J]. Plant molecular biology, 2024, 114(3):1-14. doi: 10.1007/s11103-023-01406-9
[21]	SHOJAEI E, MIRZAIE-ASL A, MAHMOUDI S B, et al. Identification of sugar beet flowering genes based on Arabidopsis homologous gene[J]. Journal of agriculture science and technology, 2017, 19:719-729.

甜菜BvFVE1过表达对抽薹开花影响的研究

Effect of BvFVE1 Overexpression on Bolting and Flowering of Sugar Beet

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 5

参考文献 21

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	佟盟露, 丁玉玲, 姜炳杉, 王子辰, 庞彩卫, 周芹. 外源抗坏血酸缓解阿特拉津对甜菜幼苗毒性及降解机理研究[J]. 中国农学通报, 2025, 41(9): 157-164.
[2]	田沁雨, 胡华兵, 王荣华, 王茂芊. L-抗坏血酸浸种对盐胁迫下甜菜种子萌发的影响[J]. 中国农学通报, 2025, 41(6): 53-59.
[3]	孙琳琳, 胡华兵, 刘建雄, 郇町, 李有芳, 刘珣, 贺碧微. 高密度甜菜覆膜与裸地营养生长规律研究[J]. 中国农学通报, 2025, 41(36): 51-59.
[4]	岳开心, 王佳琦, 刘乃新. 干旱胁迫下甜菜种子萌发响应转录组分析[J]. 中国农学通报, 2025, 41(3): 114-122.
[5]	赵鹏程, 王倍贝, 孙硕阳, 林世杰, 高宇. 新疆石河子西梅低温霜冻致灾风险评估[J]. 中国农学通报, 2025, 41(27): 118-125.
[6]	高佑凯, 宋群, 王增澔, 韦柳利, 傅奕豪, 孙阎, 孙艳春. 不同作物根际土壤细菌群落结构和多样性的分析[J]. 中国农学通报, 2025, 41(25): 60-66.
[7]	洪森荣, 汪艺, 卢亚轩, 再图妮古丽·买买提吐鲁逊, 刘李娜, 孙美龄. 甜菜碱对低温胁迫下高丹草种子萌发中呼吸电子传递及其幼苗生长的影响[J]. 中国农学通报, 2025, 41(16): 156-164.
[8]	杨家琪, 韩广源, 刘乃新, 周芹. 甜菜酪氨酸代谢途径关键酶的生物信息学研究[J]. 中国农学通报, 2024, 40(9): 124-131.
[9]	王璐洋, 胡华兵, 王荣华, 王茂芊. 磷酸二氢钠对甜菜种子引发的影响[J]. 中国农学通报, 2024, 40(33): 61-67.
[10]	仲维婷, 张琼, 兴旺, 刘大丽. 异源表达甜菜BvHSP18.2基因增强拟南芥对镉胁迫的耐受性研究[J]. 中国农学通报, 2024, 40(29): 14-20.
[11]	程梓祺, 张惠忠, 王荣华, 王茂芊. 不同浓度的维生素C对不同品种的甜菜种子引发的影响[J]. 中国农学通报, 2024, 40(26): 15-21.
[12]	赵佳, 胡晓航, 李彦丽. 甜菜糖厂滤泥与生物炭配施对东北黑土农田土壤性质和有机碳组分影响的研究[J]. 中国农学通报, 2024, 40(26): 38-45.
[13]	王望, 于泽, 崔晶晶, 宋柏权, 王秋红. 施氮对甜菜根际土壤化学性质的影响及相关性分析[J]. 中国农学通报, 2024, 40(22): 88-93.
[14]	刘爱杰, 胡华兵, 王荣华, 王茂芊. 盐胁迫对不同甜菜种子萌发特性的影响[J]. 中国农学通报, 2024, 40(21): 20-28.
[15]	李佳颖, 刘乃新, 韩广源. 红甜菜根中萜类代谢物的比较代谢组学分析[J]. 中国农学通报, 2024, 40(2): 137-142.