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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (9): 124-131.doi: 10.11924/j.issn.1000-6850.casb2023-0115

所属专题： 生物技术

• 生物科学 • 上一篇    下一篇

甜菜酪氨酸代谢途径关键酶的生物信息学研究

杨家琪1(), 韩广源2, 刘乃新1(), 周芹1   

  1. 1 黑龙江大学现代农业与生态环境学院，哈尔滨 150080
    2 哈尔滨海关技术中心，哈尔滨 150023
  • 收稿日期:2023-02-23 修回日期:2023-05-15 出版日期:2024-03-25 发布日期:2024-03-22
  • 通讯作者:
    刘乃新，女，1980年出生，黑龙江哈尔滨人，研究生导师，副研究员，研究方向：糖料作物质量安全及营养品质评价。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学农学楼，Tel：13504511205，E-mail：。
  • 作者简介:

    杨家琪，女，1998年出生，四川德阳人，研究生，研究方向：甜菜分子标记技术。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学农学楼，Tel：13408388357，E-mail：。

  • 基金资助:
    财政部和农业农村部国家现代农业产业技术体系(糖料)建设项目“质量安全与营养品质评价"(CARS-170503); 黑龙江省博士后基金“纳米硒生物强化调控寒地香稻产量和品质的分子机制研究”(LBH-Z22245)

A Bioinformatics Study on Key Enzymes Involved in Tyrosine Metabolism in Beet

YANG Jiaqi1(), HAN Guangyuan2, LIU Naixin1(), ZHOU Qin1   

  1. 1 College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
    2 Harbin Customs Technical Center, Harbin 150023
  • Received:2023-02-23 Revised:2023-05-15 Published:2024-03-25 Online:2024-03-22

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摘要：

为了了解甜菜中酪氨酸代谢途径关键酶的特性和进化关系, 以甜菜、菠菜、大豆等植物为试验材料，利用NCBI数据库收集了酪氨酸代谢途径5种关键酶(PPO、TAT、TYDC、HPPD、HPPR)的序列信息。使用ExPASy - ProtParam软件和MEGA-7软件分析了关键酶的理化性质，同时将它们序列间的相似性及演化距离进行对比，并且构建系统发育树。理化性质分析结果显示，甜菜酪氨酸代谢途径中5种关键酶均为不稳定的亲水性蛋白质，甜菜PPO的氨基酸数量最高；系统发育树分析结果显示，甜菜与菠菜的PPO和HPPD相似性高；甜菜分别与大豆的TAT、马铃薯的TYDC、大麦的HPPR相似性高。该研究为参考其他作物探索甜菜酪氨酸代谢途径关键酶功能研究提供参考，为进一步研究甜菜基因组学奠定基础。

关键词: 甜菜, 酪氨酸, 催化酶, 代谢途径, 生物信息学分析

Abstract:

To understand the characteristics and evolutionary relationships of key enzymes involved in the tyrosine metabolism pathway in sugar beets, taking plants such as sugar beet, spinach, and soybean as experimental materials, sequence information of five key enzymes in the tyrosine metabolism pathway (PPO, TAT, TYDC, HPPD, HPPR) was collected using the NCBI database. The physicochemical properties of the key enzymes were analyzed using ExPASy-ProtParam software and MEGA-7 software, their sequence similarities and evolutionary distances were compared, and a phylogenetic tree was constructed. The analysis of physicochemical properties showed that all five key enzymes in the tyrosine metabolism pathway of sugar beet were unstable hydrophilic proteins, and the number of amino acids in sugar beet PPO was the highest; the phylogenetic tree analysis results showed that there was high similarity in PPO and HPPD between sugar beet and spinach; beets had high similarity with TAT of soybeans, TYDC of potatoes, and HPPR of barley, respectively. This study provided a reference for other crops to explore the key enzyme function of sugar beet tyrosine metabolism pathway and laid a foundation for further research on sugar beet genomics.

Key words: beet, tyrosine, catalytic enzyme, metabolic pathway, bioinformatics analysis