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中国农学通报

中国农学通报 ›› 2023, Vol. 39 ›› Issue (30): 36-46.doi: 10.11924/j.issn.1000-6850.casb2022-0805

• 农学·农业基础科学 • 上一篇    下一篇

褪黑素和多巴胺对甜菜种子引发与盐胁迫的比较研究

杨然(), 兴旺, 刘大丽, 吴则东, 王茂芊()   

  1. 黑龙江大学现代农业与生态环境学院，哈尔滨 150080
  • 收稿日期:2022-09-26 修回日期:2023-08-28 出版日期:2023-10-25 发布日期:2023-10-19
  • 通讯作者: 王茂芊，女，1983年出生，辽宁人，副研究员，博士，研究方向：种子引发和甜菜遗传育种。通信地址：150080 黑龙江省南岗区学府路74号 黑龙江大学现代农业与生态环境学院，Tel：0451-86604650，E-mail：haixiang80@126.com。
  • 作者简介:

    杨然，女，1998年出生，江苏连云港人，硕士，研究方向：甜菜种子引发和遗传育种。通信地址：150080 黑龙江省南岗区学府路74号 黑龙江大学现代农业与生态环境学院，Tel：0451-86604650，E-mail：。

  • 基金资助:
    国家糖料产业体系项目“甜菜种质资源鉴定与新种质创制”(CARS-170102); 国家糖料产业体系子项目“甜菜种子引发与检测技术研究”(CARS-17011306); 国家青年科学基金项目“能源甜菜应答重金属Cd逆境胁迫分子机制的转录组学研究及差异表达基因功能鉴定”(31601229); 黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057); 黑龙江省省属高等学校基本科研业务费基础研究项目“不同根冠比甜菜品种的生理特性及转录组-蛋白组联合分析研究”(2021-KYYWF-0022); 国家自然基金面上项目“转录因子BvERF61调控甜菜耐盐分子机制研究”(32272148); 黑龙江省博士后面上项目“不同根冠比甜菜转录组-蛋白组联合分析”(LBH-Z22246)

Comparative Study of Melatonin and Dopamine on Sugar Beet Seed Initiation and Salt Stress

YANG Ran(), XING Wang, LIU Dali, WU Zedong, WANG Maoqian()   

  1. College of Advanced Agriculture and Ecological Environment of Heilongjiang University, Harbin 150080
  • Received:2022-09-26 Revised:2023-08-28 Published-:2023-10-25 Online:2023-10-19

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摘要：

旨在探究褪黑素和多巴胺对不同类型甜菜种子引发与盐胁迫的对比。研究以TD801和TD805两种甜菜种子为试验材料，在非盐胁迫和盐胁迫的条件下，采用了2种引发剂、6种浓度梯度和2种时间，共设置了96组处理，通过对TD801和TD805两种甜菜种子浸泡、回干以及发芽培养等步骤，来测定它们的发芽势、发芽率、发芽指数以及活力指数等发芽指标。结果显示，大部分处理组合的发芽率、发芽势、发芽指数和活力指数显著高于对照。TD801甜菜种子在500 μmol/L浓度的褪黑素溶液，且处理时间为24 h的组合下引发效果最优，发芽率和发芽势高达90.00%；盐胁迫在特定组合下能够转变成盐引发，对甜菜种子的发芽有促进作用。褪黑素和多巴胺对甜菜种子的发芽有一定的引发效果，两者相比，褪黑素萌发效果显著优于多巴胺。

关键词: 甜菜种子, 褪黑素, 多巴胺, 种子引发, 盐胁迫

Abstract:

The aim was to explore the effects of melatonin and dopamine on different types of beet seed initiation and salt stress. In this study, two kinds of beet seeds, TD801 and TD805, were used as experimental materials. Under the condition of non-salt stress and salt stress, two initiators, six different gradient concentrations and two different processing times were used to set up 96 groups of treatments. The germination potential, germination rate, germination index and vitality index and other germination indicators were determined through soaking, re-drying and germination culture of TD801 and TD805 beet seeds. The results showed that the germination rate, germination potential, germination index and vitality index of most treatment combinations were significantly higher than those of control. TD801 beet seeds were treated with 500 μmol/L melatonin solution for 24 h, and the germination rate and germination potential were up to 90.00%. Salt stress could be converted into salt initiation under specific combination, which promoted the germination of beet seeds. Melatonin and dopamine have a certain effect on the germination of beet seeds, and the germination effect of melatonin is significantly better than that of dopamine.

Key words: sugar beet seed, melatonin, dopamine, seed initiation, salt stress