外源腐胺对盐胁迫下菜豆种子萌发及抗性的影响

doi:10.11924/j.issn.1000-6850.casb2022-0719

摘要/Abstract

摘要：

为了探究外源腐胺对盐胁迫下菜豆种子萌发的影响，本研究以菜豆品种‘P16028’为试验材料，设置蒸馏水对照(CK)、100 mmol/L NaCl处理(NaCl)以及NaCl处理条件下添加1 mmol/L Put浸种(NaCl + 1 Put) 3个处理组，对其发芽势、发芽率、发芽指数及生理指标进行测定。结果表明：与CK相比，盐胁迫导致菜豆种子发芽势、发芽率、发芽指数显著降低，抗氧化酶SOD、POD、CAT均呈下降趋势，其O₂^-、MDA、可溶性蛋白、可溶性糖含量和脯氨酸含量上升，种子萌发受到抑制。而添加外源Put显著提高了盐胁迫下菜豆种子发芽势、发芽率、发芽指数，也提高了SOD、POD、CAT氧化酶活性，降低其O₂^-和MDA含量，提升渗透调节物质含量，提高菜豆对盐胁迫的抗性。由此可见，使用外源Put浸种可以缓解盐胁迫带来的伤害，提高菜豆种子的耐盐性，从而有效的促进菜豆种子的萌发。

关键词: 菜豆, 腐胺, 萌发, 盐胁迫, 种子

Abstract:

In order to study the effects of exogenous Put on seed germination of snap bean under salt stress, the snap bean variety ‘P16028’ was used as experimental material. Three treatments, distilled water control (CK), 100 mmol/L NaCl treatment (NaCl) and addition of 1 mmol/L Put under NaCl treatment (NaCl + 1 Put) were used to determine the germination potential, germination rate, germination index and physiological index. The results showed that compared with CK, salt stress led to a significant decrease of the germination potential, germination rate, germination index, and a downward trend of the activities of antioxidant enzymes SOD, POD and CAT. The contents of O₂^-, MDA, soluble protein, soluble sugar and proline increased, and seed germination was inhibited. The addition of exogenous Put significantly increased the germination potential, germination rate and germination index under salt stress, and also increased the activities of antioxidant enzymes SOD, POD and CAT, reduced the contents of O₂^- and MDA, increased the content of osmoregulatory substances, and improved the resistance of bean to salt stress. Therefore, the application of exogenous Put can alleviate the damage caused by salt stress and improve the salt tolerance of snap bean seeds, thus effectively promoting the germination of snap bean seeds.

Key words: snap bean, Put, germination, salt stress, seeds

胡竞渝, 冯国军, 刘大军, 杨晓旭, 闫志山, 刘畅. 外源腐胺对盐胁迫下菜豆种子萌发及抗性的影响[J]. 中国农学通报, 2023, 39(15): 52-58.

HU Jingyu, FENG Guojun, LIU Dajun, YANG Xiaoxu, YAN Zhishan, LIU Chang. Effects of Exogenous Put on Seed Germination and Resistance of Snap Bean Seeds Under Salt Stress[J]. Chinese Agricultural Science Bulletin, 2023, 39(15): 52-58.

图/表 9

处理	发芽势/%	发芽率/%	发芽指数
CK	87.33±1.15a	94.67±1.15a	72.99±1.37a
NaCl	51.33±7.02b	64.00±5.29b	41.11±3.38c
NaCl + 1 Put	85.33±1.15a	90.67±3.06a	67.09±1.05b

表1. 外源Put对盐胁迫下菜豆种子萌发影响

图1. Put处理对盐胁迫下菜豆种子SOD活性的影响

图2. Put处理对盐胁迫下菜豆种子POD活性的影响

图3. Put处理对盐胁迫下菜豆种子CAT活性的影响

图4. Put处理对盐胁迫下菜豆种子O2-产生速率的影响

图5. Put处理对盐胁迫下菜豆种子丙二醛含量的影响

图6. Put处理对盐胁迫下菜豆种子可溶性蛋白质含量的影响

图7. Put处理对盐胁迫下菜豆种子可溶性糖含量的影响

图8. Put处理对盐胁迫下菜豆种子脯氨酸含量的影响

参考文献 39

[1]	姚荣江, 杨劲松, 刘广明. 东北地区盐碱土特征及其农业生物治理[J]. 土壤, 2006(3):256-262.
[2]	刘爱平. 盐碱胁迫下星星草幼苗离子平衡的调节机制研究[D]. 扬州: 扬州大学, 2007.
[3]	石德成, 赵可夫. NaCl和Na₂CO₃对星星草生长及营养液中主要矿质元素存在状态的影响[J]. 草业学报, 1997, 6(2):52-62
[4]	常汝镇, 陈一舞, 邵桂花, 等. 盐对大豆农艺性状及籽粒品质的影响[J]. 大豆科学, 1994(2):101-105.
[5]	罗庆云, 於丙军, 刘友良. 大豆苗期耐盐性鉴定指标的检验[J]. 大豆科学, 2001(3):177-182.
[6]	朱新广, 张其德. NaCl对光合作用影响的研究进展[J]. 植物学通报, 1999(4):332-338.
[7]	张永锋, 梁正伟, 隋丽, 等. 盐碱胁迫对苗期紫花苜蓿生理特性的影响[J]. 草业学报, 2009, 18(4):230-235.
[8]	李霞, 程运河, 马晓东, 等. 多胺在植物抗逆中的生理机制[J]. 世界林业研究, 2018, 31(4):23-28.
[9]	胡景江, 左仲武. 外源多胺对油松幼苗生长及抗旱性的影响[J]. 西北林学院学报, 2004, 19(4):5-8.
[10]	SHEN W, NADA K, TACHIBANA S. Involvement of polyamines in the chilling tolerance of cucumber cultivars[J]. Plant physiology, 2000, 124(1):431-439. pmid: 10982456
[11]	田婧. 外源亚精胺缓解黄瓜幼苗高温胁迫伤害的生理调节机制和蛋白质组学研究[D]. 南京: 南京农业大学, 2012:153.
[12]	BITOCCHI E, NANNI L, BELLUCCI E, et al. Mesoamerican origin of the common bean (Phaseolus vulgaris L.) is revealed by sequence data[J]. Proceedings of the national academy of sciences, 2012, 109(14):5788-5796.
[13]	冯国军, 刘大军. 菜豆的营养价值评价与分析[J]. 北方园艺, 2016(24):200-208.
[14]	陈琼, 韩瑞玺, 唐浩, 等. 我国菜豆新品种选育研究现状及展望[J]. 中国种业, 2018, 283(10):19-24.
[15]	刘珂, 张嘉欣, 杜清洁, 等. 外源褪黑素对盐胁迫下香椿种子萌发及幼苗生长的影响[J]. 中国瓜菜, 2020, 33(5):53-58.
[16]	刘月, 寇从贤, 付桂萍, 等. 褪黑素对大豆幼苗盐害的缓解效应及机理研究[J]. 中国油料作物学报, 2017, 39(6):813-819.
[17]	孙浩月. 盐胁迫对普通菜豆(Phaseolus vulgaris L.)萌发期生长的影响及其耐盐性分子机制[D]. 大庆: 黑龙江八一农垦大学, 2021.
[18]	GIANNOPOLITIS C, RIES S. Superoxide dismutases: I. occurrence in higher plants[J]. Plant physiology, 1977, 59(2):309-314. doi: 10.1104/pp.59.2.309 pmid: 16659839
[19]	THOMAS R, JEN J, MORR C. Changes in solule and ound peroxidase—IAA oxidase during tomato fruit development[J]. J Food science, 1981, 47:158-161. doi: 10.1111/jfds.1982.47.issue-1 URL
[20]	CAKMAK I, MARSCHNER H. Magnesium deficiency and high light intensity enhance activities of superoxide dismutase, ascorbate peroxidase, and glutathione reductase in bean leaves[J]. Plant physiology, 1992, 98(4):1222-1227. doi: 10.1104/pp.98.4.1222 pmid: 16668779
[21]	DHINDSA R, PULMB-DHNDSA P, THORPE T. Leaf senescence: Correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase[J]. Journal of experimental botany, 1981, 32(126):93-101. doi: 10.1093/jxb/32.1.93 URL
[22]	ELSTNER E, HEUPEL A. Inhibition of nitrite formation from hydroxylammonium chloride: a simple assay for superoxide dismutase[J]. Analytical biochemistry, 1976, 70(2):616-620. doi: 10.1016/0003-2697(76)90488-7 URL
[23]	张志良. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2003:268-269.
[24]	李合生. 植物生理生化实验原理和技术[M]. 北京: 商等教育出版社, 2003.
[25]	张凤银, 陈禅友, 胡志辉, 等. 外源水杨酸对盐胁迫下菜豆种子萌发和幼苗生理特性的影响[J]. 东北农业大学学报, 2013, 44(10):39-43.
[26]	孙宗玖, 李培英, 阿不来提, 等. 种子萌发期38份偃麦草种质耐盐性评价[J]. 草业科学, 2012, 29(7):1105-1113.
[27]	王晓宁, 何新蕾, 樊滢玮, 等. 盐胁迫对甜高粱种子萌发及幼苗抗氧化酶活性的影响研究[J/OL]. 分子植物育种:1-8[2022-07-30].
[28]	王建丽, 牟林林, 申忠宝, 等. NaCl胁迫对籽粒苋种子萌发的影响[J]. 黑龙江农业科学, 2022(1):14-19.
[29]	张铖锋, 李硕, 石玉, 等. 腐胺浸种对Ca(NO₃)₂胁迫下不同品种黄瓜幼苗生长及生理抗性的影响[J]. 山东农业科学, 2019, 51(12):67-72.
[30]	RASKIN I. Role of salicylic acid in plant[J]. Annual review of plant physiology and plant molecular biology, 1992, 43:439-463. doi: 10.1146/arplant.1992.43.issue-1 URL
[31]	张润花. 外源多胺对黄瓜幼苗盐胁迫生理响应的影响[D]. 南京: 南京农业大学, 2009.
[32]	华智锐, 李小玲. 外源腐胺对盐胁迫下黄芩根中活性氧代谢的影响[J]. 山西农业科学, 2017, 45(11):1766-1769,1793.
[33]	王强, 尹相博. 不同种类外源多胺缓解大豆盐胁迫伤害的研究[J]. 河南农业科学, 2014, 43(4):48-50,55.
[34]	周旋. 盐胁迫下茶树对外源水杨酸的生理响应[D]. 杨凌: 西北农林科技大学, 2014.
[35]	韩志平. 盐胁迫对小型西瓜生长、生理代谢的影响及外源钙和腐胺的缓解效应研究[D]. 南京: 南京农业大学, 2008.
[36]	EI-SAMAD H, SHADDAD M. Salt tolerance of soybean cultivars[J]. Biologia plantarum, 1997, 39(2):263-269. doi: 10.1023/A:1000309407275 URL
[37]	阮成江, 谢庆良. 盐胁迫下沙棘的渗透调节效应[J]. 植物资源与环境学报, 2002, 11(2):45-47.
[38]	AZIZ A, MARTIN -TANGGU J, LARHER F. Salt -induced changes in polyamine and tyramine levels can regulate proline accumulation in tomato leaf discs treated with sodium chloride[J]. Physiologia plantarum, 1998, 104:195-202. doi: 10.1034/j.1399-3054.1998.1040207.x URL
[39]	SINGH D, VARMA S, MISHRA S. Putrescine effect on nitrate reductase activity, organic nitrogen, protein, and growth in heavy metal and salinity stressed mustard seedlings[J]. Biologia plantarun, 2002, 45(4):605-608.