Abstract: An experiment was conducted to investigate the effects of different levels of Flavomycin supplement on growth performance and activities of digestive enzymes in Cyprinus carpio. The results showed: The biology synthesis evaluates in the groups fed diets with Flayomycin were higher and that (119) in the group fed diets with 6mg/kg Flayomycin was the highest. Feed coefficients in the groups fed diets with 4mg/kg and 6mg/kg Flayomycin were significantly higher than that in the control group (P<0.05). The activities of trypsin and intestinal protease were the highest in the group fed diets with 4mg/kg Flayomycin. The activities of trypsin in the group fed diets with 4mg/kg Flayomycin were significantly higher than those in the control group and the group fed diets with 8mg/kg Flayomycin (P<0.05). The activities of intestinal protease in the group fed diets with 4mg/kg and 6mg/kg Flayomycin were significantly higher than those in the control group (P<0.05). The amylase activities in hepatopancreas in the groups fed diets with 2mg/kg, 4mg/kg, 6mg/kg Flayomycin were significantly higher than those in the control group (P<0.05). The amylase activities in intestine in the group fed diets with 4mg/kg Flayomycin were significantly higher than those in the control group (P<0.05). The lipase activities in hepatopancreas and intestine in the group fed diets with 6mg/kg Flayomycin were significantly higher than those in the control group (P<0.05).