绿色荧光蛋白基因在植病研究中的应用

摘要/Abstract

摘要： 综述了绿色荧光蛋白基因在植物病害研究中的应用及进展。重点分析了绿色荧光蛋白基因在病原菌的检测、侵染与定殖、致病基因的表达及构建抗性植株的筛选中的应用，提出了绿色荧光蛋白基因在应用中存在的问题以及在植物病害研究领域展现出的巨大潜力。

关键词: 苏州, 苏州, 农村基本养老保险, 现状分析, 对策

Abstract: This article reviews the prospect and applications of the green fluorescent protein (GFP) gene in the study of plant disease, especially emphasis on the applications of GFP in pathogen detection, infection and localization, the expression of genes, and selecting for transgenic plant. This paper comes up with the problems existing about the applications of GFP and promises a great potential in modern plant disease research field.

中图分类号:

S432.4

王震,,郭爱玲,冯莉. 绿色荧光蛋白基因在植病研究中的应用[J]. 中国农学通报, 2007, 23(6): 493-493.

Wang Zhen,, Guo Ailing, Feng Li. Applications of Green Fluorescent Protein Gene in the Study of Plant Disease[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 493-493.

参考文献

[1] Shimomura O, Johnson F H, Saiga Y. Extraction purification and properties of a bioluminescent protein from the luminous hudrmedusan, Aequorea. J Cell Comp Physiol, 1962,59:223-240.
[2] Chalfie M, Ta Y, Euskirchen G, et al. Green fluorescent protein as a marker for gene expression [J]. Science, 1994,263:802-805.
[3] Cormack B P, Baldivia R H, Falkow S. FACS-optimized mutants of the green fluorescent protein(GFP). Gene, 1996,173:33-38.
[4] CrameriA, Whitehorn E A, Tate E, et al. W P C. Improved green fluorescent protein by molecular evolution using DNA shuffling. Nature Biotech, 1996,14:315-319.
[5] Heim R, Prasher D C, Tsien R Y. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Natl Acad Sci USA, 1994, 91:12501-12504.
[6] Eberl L, Schulze R, Ammendola A, et al. Use of green fluorescent protein as a marker for ecological studies of activated sludge communities. FFMS Microbiol Lett, 1997,149:77-83.
[7] Chiu W, Ntva Y, Zeng W, et a1.Engineered GFP as a Vital Reporter in Plants. Curr, Bio1, l997,6:325-330.
[8] Hu W, Cheng C L. Expression of Aequorea green fluorescent protein in plant cells. FEBS Letters, l995,369:331-334.
[9] Ponappa T, Brzozowaki A E, Finer J J. Transient expression and stable transformation of soybean using the jellyfish green fluorescent protein[J]. Plant Cell Rep, 1999,19(1):6-12.
[10] Gerard J A R, Odette M, Emill J H W, et al. Enhanced expression in tobacco of the gene encoding green fluorescent protein by modification of its codon usage. Plant Molecular biology, 1997,33:989-999.
[11] Burlage R S, Sayler G S, et al. Monitoring of naphthalene cataboliam by bioluminescence with nah-lux transcriptional fusion. Bacterial, 1990,172:4749-4757.
[12] Selifonova O, Burlage R, et al. Biolumnescence sensor for the detection of bioavailable Hg in the environment. Appl Environ Microbiol, 1993,59:3083-3090.
[13] Mitra Kooshki, Ayalew Mentewab, et al. Pathogen inducible reporting in transgenic tobacco using a GFP construct. Plant Science, 2003,165:213-219.
[14] Heinlein, et al. Interaction of tobamovirus movement proteins with the plant cytoskeleton. Science, 1995,270:1983-1985.
[15] Epel B L, Padgett H S, et al. Plant virus movement protein dynamics probed with a GFP-protein fusion [J]. Gene, 1996,173:75-79.
[16] 郝敏,邢达,周俊初.含有绿色荧光蛋白基因的质粒载体的构建及其在水稻白叶枯细菌中的表达.激光生物学报,2002,11(6):427-430.
[17] Bloemberg G V, OTooe G A, Lugtenberg B J J, et al. Green fluorescence protein as a marker for Pseudomonas spp. Appl Environ Microbiol, 1997,63:4543-4551.
[18] Normander B, Christensen B B, Molin S, et al. Effect of bacterial distribution and activity on conjugal gene transfer on the phylloplane of the bush bean (Phaseolus vulgaris). Appl Environ Microbiol, 1998,64:1902-1909.
[19] 张昕,张炳欣,喻景权.生防菌ZJY－1及ZJY－116的GFP标记及其在黄瓜根围的生态适应性.应用生态学报,2005,16(11):2144-2148.
[20] 吕泽勋,李久蒂,朱至清.用绿色荧光蛋白基因 (gfp)标记产酸克雷伯氏菌SG-11研究其在水稻苗期根部的定殖.农业生物技术学报,2001,9(1):13-18.
[21] Weinstein M, Roberts R C , Helinski D R. A region of the broad-host-range plasmid RK2 causes stable in planta inheritance of plasmids in Rhizobium meliloti cells isolated from Alfalfa root nodules. J Bacteriol, 1992, 174:7486-7489.
[22] Dunn A K , Handelsman J. A vector for promoter trapping in Bacillus cereus. Gene, 1999,226:297-305.
[23] Peter J, Gregory D, et al. Development of a transformation system for Mycosphaerella pathogens of banana: a tool for the study of host/pathogen interactions. FEMS Microbiology Lett, 2001,195:9-15.
[24] Xi C, Lanbrecht M, Vanderleyder I, et al. Bi-functional gfp-and gusA-containing mini-Tn5 transposon derivatives for combined gene expression and bacterial localization studies. J. Micro. Methods.1999,35:85-92.
[25] Errampalli D, Trevors J T, et al. Green fluorescent protein as a marker to monitor survival of phenanthrene-Mineralizing Pseudomonas sp. UG14Gr in creosote-contaminated soil. FEMS Microbiol Ecol.1998, 26:181-191.
[26] Matz M V, Fradkov F, Labas Y A, et al. Fluorescent proteins from nonbioluminescent Anthozoa species. Nature Biotechnology, 1999, 17 (10):969-973.
[27] Yanushevich Y G, Staroverov D B, Savitsky A P, et al. A strategy for the generation of non-aggregating mutants of Anthozoa fluorescent proteins. FEBS Lett, 2002,511(13):11-14.