欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (8): 40-40.

所属专题： 生物技术； 畜牧兽医

• 目次 • 上一篇    下一篇

绵羊regakine-1基因的克隆与序列分析

乔新安,杨国宇,王艳玲,王月影,韩立强,都军霞   

  • 出版日期:2007-08-05 发布日期:2007-08-05

Molecular Cloning and Sequence Analysis of Ovine Regakine-1

Qiao Xinan, Yang Guoyu, Wang Yanling, Wang Yueying, Han Liqiang , Du Junxia   

  • Online:2007-08-05 Published:2007-08-05

PDF (PC)

634

摘要： 【研究目的】克隆并分析绵羊regakine-1基因；【方法】肠系膜淋巴结组织提取总RNA，利用设计的引物进行RT-PCR，PCR产物与pMD-19T载体连接后转化JM109感受态细胞，筛选阳性克隆、测序，并进行序列分析；【结果】克隆的绵羊regakine-1基因与牛的同源性为91%，推测的氨基酸序列信号肽为1~21aa，SCY结构域为29~87aa，结构特征与牛的相一致。【结论】克隆了绵羊regakine-1基因的ORF，并注册GenBank (AccessionEF617337)

关键词: 陕北农牧交错带, 陕北农牧交错带, 粮食安全, 植物性产品, 动物性产品

Abstract: 【Objective】To clone and analysis the ovine regakine-1.【Method】Total RNA was extracted from mesenteric lymph node and mRNA sequence of gene were amplified by RT-PCR with designed primers. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of JM109. The recombinant plasmid was identified and the sequence was analyzed.【Results】Identity analysis showed that the regakine-1 nucleotide sequence shared 91% homology with that of cattle. The signal peptide of predicted amino acid sequence is 1~21aa and the structural domain SCY is 29~87aa, revealing that the structural feature is consistent with cattle regakine-1.【Conclusion】The ovine ORF of regakine-1 gene was successfully cloned in present study and the sequence has been submitted to GenBank(Accession. EF617337).

中图分类号: