关键词: 兰花选育种, 兰花选育种, 种质资源, 传统育种, 生物技术

Abstract: The micro-encapsulation of pumpkin seed oil extracted by supercritical CO2 was studied by spray-drying method， and the structure of the product was observed by microscope as well as the products were studied by antioxidation experiment. The result indicated that the order of the effects on the micro-encapsulation efficiency for the micro-encapsulation prescription to the pumpkin seed oil from the greatest to the least was the ratio of the wall[soybean protein isolation(SPI)/malt dextrin(MD)], the ratio of the core to wall and the total soluble solid(TSS). The optimal conditions from orthogonal experimental design were as follows, SPI to MD was 2:1(W/W)， the ratio of core to wall was 1:5(W/W)， TSS was 25%(W/V). The order of the effects on the micro-encapsulation efficiency for the micro-encapsulation technological parameters from the greatest to the least were temperatures of entering wind, temperatures of out wind and homogenizing pressure. The optimal spray-drying technological parameters from orthogonal experimental design were as follows, temperatures of entering wind and out wind were 180℃ and 80℃ respectively, homogenizing pressure was 30MPa. The micro-encapsulation efficiency of the production was 90.2%, and the products of pumpkin seed oil microcapsules are in good antioxidation and microcosmic structure produced by using the optimal micro-encapsulation prescription and spray-drying technological parameters.