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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (9): 452-456.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

不同SDS-PAGE分离胶浓度下椰子贮藏
蛋白亚基的分离效果

郑亚军,李 艳,李新菊,郑小蔚,覃伟权   

  • 收稿日期:2008-06-06 修回日期:2008-07-02 出版日期:2008-09-05 发布日期:2008-09-05

The Effect of Coconut Storage Protein Subunits under Different Separation
Gel Concentrations of SDS-PAGE

Zheng Yajun, Li Yan, Li Xinju, Zheng Xiaowei, Qin Weiquan   

  • Received:2008-06-06 Revised:2008-07-02 Online:2008-09-05 Published:2008-09-05

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摘要： 采用SDS-PAGE浓缩胶浓度为7.5%，分离胶浓度分别为9%、10%、11%、11.5%、12%、13%、14%、15%、16%的凝胶系统，探讨不同分离胶浓度条件下椰子贮藏蛋白亚基的分离效果。结果表明，分离胶浓度对亚基电泳分辨率的影响非常明显。分离胶浓度为11%~12%时，蛋白质亚基的分辨效果很好；当分离胶浓度为11.5%时，椰子贮藏蛋白亚基的分离效果最好，可以得到9条清晰的亚基条带。

Abstract: This experiment attempted to research the separating effect of coconut storage protein subunits under different separation gel concentrations of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that the separating effect of coconut storage protein subunits could be greatly affected by the concentration change of separation gel. The good separating effect of coconut storage protein could be obtained when the separation gel concentration was 11% to 12%. The resolving effect of coconut protein subunits was best when the separation concentration was 11.5%.

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