Abstract: Cassava was the main crop rich in starch, but with high content of the toxic cyanogenic glucosides linamarin and lotaustralin in all tissues, and cytochrome P450 gene catalyzing the biosynthesis of cyanogenic glucoside. In this study, the complete coding sequence (CDS) of N-hydroxylating cytochrome P450 (CYP79D2) gene, which catalyzing the committed enzyme in the first step in the biosynthesis of cyanogenic glucoside, was cloned by RT-PCR from cassava (Manihot esculenta Crantz). Analysis showed the cloned gene coding 541 deduced amino acids, and shared 99% homology both in nucleotide acid sequence and in amino acid sequence compared with other CYP79D2 isolated from cassava, and the gene include conserve domain in accordance with other members of the CYP79D2 gene family.