油菜B-box 型锌指蛋白基因BnSTO 的克隆和表达

doi:10.11924/j.issn.1000-6850.casb16020056

中国农学通报 ›› 2016, Vol. 32 ›› Issue (36): 96-103.doi: 10.11924/j.issn.1000-6850.casb16020056

所属专题：生物技术；油料作物；园艺

油菜B-box 型锌指蛋白基因BnSTO 的克隆和表达

胡茂龙,龙卫华,高建芹,张洁夫,陈松,浦惠明

(江苏省农业科学院经济作物研究所/农业部长江下游棉花与油菜重点实验室/江苏省现代作物生产协同创新中心,南京 210014）

收稿日期:2016-02-18 修回日期:2016-12-11 接受日期:2016-04-22 出版日期:2016-12-26 发布日期:2016-12-26
通讯作者: 胡茂龙
基金资助:
国家现代农业产业技术体系岗位科学类项目“长江下游区油菜育种”(CARS-13)；农业部948项目“基于油菜农艺性状定位信息开发高通量SNP芯片引进”(2011-G23)；江苏省农业科技自主创新资金“江苏滩涂盐碱地耐盐经济作物高效种植关键技术创新与示范”[CX(13)3051]。

Cloning and Expression of BnSTO Encoding B-box-type Zinc-finger Proteins in Rapeseed

Hu Maolong, Long Weihua, Gao Jianqin, Zhang Jiefu, Chen Song, Pu Huiming

(Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed (Nanjing), Ministry of Agriculture/Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing 210014)

Received:2016-02-18 Revised:2016-12-11 Accepted:2016-04-22 Online:2016-12-26 Published:2016-12-26

摘要/Abstract

摘要： B-box型锌指蛋白参与植物多种生长发育和非生物逆境胁迫响应过程。笔者通过RT-PCR克隆得到甘蓝型油菜2个B-box型锌指蛋白基因BnSTO-1和BnSTO-2。BnSTO-1的ORF长度为723 bp，推测编码240个氨基酸，定位于A8染色体。BnSTO-2的ORF长度为726 bp，推测编码241个氨基酸，定位于C8染色体。2个基因均由3个外显子和2个内含子构成，在氨基酸水平上的相似性达到了96.68%。系统进化分析表明BnSTO与拟南芥AtSTO的亲缘关系较近，同源性较高。荧光实时定量PCR结果表明BnSTO在检测的各个组织中均有表达，其中叶和蕾中的表达量最高；高盐、干旱和高温能显著诱导BnSTO-1和BnSTO-2的表达，但出现峰值的时间不同，推测2个基因均可能参与了油菜的非生物逆境胁迫响应。

关键词: 冬小麦, 冬小麦, 抗倒伏指数, 产量, 生长调节剂

Abstract: The B-box-type zinc finger proteins play crucial roles in regulating plant growth and development, and response to abiotic stresses in plants. In this paper, two genes BnSTO-1 and BnSTO-2, encoding B-box-type zinc finger proteins, were cloned from rapeseed (Brassica napus L.) by reverse transcription PCR (RT-PCR). The gene BnSTO-1 contained an open reading frame (ORF) of 723 bp, encoding a peptide of 240 amino acid residues. It was mapped on chromosome A8. The ORF of BnSTO-2 was 726 bp in length, encoding a peptide of 241 amino acid residues. It was mapped on chromosome C8. Sequence analysis of the genomic fragment demonstrated that the two BnSTOs genes consisted of three exons separated by two introns. BnSTO-1 and BnSTO-2 shared 96.68% amino acid sequence homology. Phylogenetic analysis revealed that deduced BnSTO-1 and BnSTO-2 proteins were close to AtSTO protein in Arabidopsis. Real-time quantitative PCR showed that BnSTO-1 and BnSTO-2 were both expressed in all tissues tested, with the highest expression in leaves and buds; their expressions were significantly increased at different time in treatments of salt, drought, and high temperature, suggesting BnSTO genes were putatively associated with the responses of abiotic stresses.

中图分类号:

Q781

胡茂龙,龙卫华,高建芹,张洁夫,陈松,浦惠明. 油菜B-box 型锌指蛋白基因BnSTO 的克隆和表达[J]. 中国农学通报, 2016, 32(36): 96-103.

Hu Maolong,Long Weihua,Gao Jianqin,Zhang Jiefu,Chen Song and Pu Huiming. Cloning and Expression of BnSTO Encoding B-box-type Zinc-finger Proteins in Rapeseed[J]. Chinese Agricultural Science Bulletin, 2016, 32(36): 96-103.

参考文献

[1] Sun S J, Guo S Q, Yang X, et al. Functional analysis of a novel Cys2/His2-type zinc finger protein involved in salt tolerance in rice[J]. J Exp Bot, 2010, 61(10):2807-2818.
[2] Chen W J, Zhu T. Networks of transcription factors with roles in environmental stress response[J]. Trends Plant Sci, 2004, 9(12): 591-596.
[3] Nakashima K, Yamaguchi-Shinozaki K. Regulations involved in osmotic stress responsive and cold stress-responsive gene expression in plants[J]. Physiol Plant, 2006, 126(1):62-71.
[4] Sakamoto H, Maruyama K, Sakuma Y, et al. Arabidopsis Cys2/His2-type zinc finger proteins function as transcription repressors under drought, cold and high-salinity stress conditions[J]. Plant Physiol, 2004, 136(1): 2734-2746.
[5] Takatsuji H. Zinc-finger proteins: the classical zinc finger emerges in contemporary plant science[J]. Plant Mol Biol, 1999, 39(6):1073-1078.
[6] Huang J, Wang M M, Jiang Y, et al. Expression analysis of rice A20/AN1-type zinc finger genes and characterization of ZFP177 that contributes to temperature stress tolerance[J]. Gene, 2008, 420(2):135-144.
[7] Huang J, Wang J F, Zhang H S. Rice ZFP15 gene encoding for a novel C2H2-type zinc finger protein lacking DLN box, is regulated by spike development but not by abiotic stresses[J]. Mol Biol Rep, 2005, 32(3):177-83.
[8] Ciftci-Yilmaz S, Mittler R. The zinc finger network of plants[J].Cell Mol Life Sci,2008,65(7): 1150-1160.
[9] Wang Q M, Zeng J X, Deng K Q, et al. DBB1a, involved in gibberellin homeostasis, functions as a negative regulator of blue light-mediated hypocotyl elongation in Arabidopsis[J]. Planta, 2011,233(1):13-23.
[10] Klug A, Schwabe J. Protein motifs 5 zinc fingers[J].the FASEB J,1995, 9(8):597-604.
[11] Datta S, Hettiarachchi C, Johansson H, et al. Salt tolerance homolog, a B-Box protein in Arabidopsis that activates transcription and positively regulates light-mediated development[J]. The Plant Cell, 2007,19(10):3242-3255.
[12] Indorf M, Cordero J, Neuhaus G, et al. Salt tolerance (STO), a stress-related protein, has a major role in light signaling[J]. The Plant J, 2007, 51(4):563-574.
[13] Khanna R, Kronmiller B, Maszle D R, et al. The Arabidopsis B-box zinc finger family[J]. The Plant Cell, 2009, 21(11):3416-3420.
[14] Lippuner V, Cyert M S, Gasser C S. Two classes of plant cDNA clones differentially complement yeast calcineurin mutants and increase salt tolerance of wild type yeast[J]. J Biol Chem, 1996, 271 (22): 12859-12866.
[15] Nagaoka S, Takano T. Salt tolerance-related protein STO binds to a Myb transcription factor homologue and confers salt tolerance in Arabidopsis[J]. J Exp Bot, 2003, 391(54):2231-2237.
[16] 刘焱,邢立静,李俊华,等.水稻含有B-box锌指结构域的OsBBX25蛋白参与植物对非生物胁迫的响应[J].植物学报,2012,47(4):366-378.
[17] Huang J Y, Zhao X B, Weng X Y, et al. The rice B-box zinc finger gene family: Genomic identification, characterization,expression profiling and diurnal analysis[J]. PloS one, 2012, 7(10):e48242.
[18] 宁露云,李蓓,李佩,等.矮牵牛B-box型锌指蛋白基因PhBBX8的克隆与表达分析[J].园艺学报,2014,41(12):2437-2445.
[19] 高尔娣,陆续,沈乾,等.青蒿B-BOX型锌指蛋白基因AaBBX22的克隆及表达分析[J].上海交通大学学报:农业科学版,2013,31(5):20-25.
[20] 王汉中.中国油菜生产抗灾减灾技术手册[M].北京:中国农业科学技术出版社,2009:1-2.
[21] 童晋,詹高淼,王新发,等.油菜柠檬酸合酶基因的克隆及在逆境下的表达[J].作物学报,2009,35(1):33-40.
[22] Murray M G, Thomspon W F. Rapid isolation of high molecular weight plant DNA [J]. Nucleic Acids Research,1980,8(19):4321-4326.
[23] Niu Y, Wu G Z, Ye R, et al. Global analysis of gene expression profiles in Brassica napus developing seeds reveals a conserved lipid metabolism regulation with Arabidopsis thaliana[J]. Mol Plant, 2009, 2(5):1107-1122.
[24] Hu M L, Pu H M, Kong L N, et al. Molecular characterization and detection of a spontaneous mutation conferring imidazolinone resistance in rapeseed and its application in hybrid rapeseed production[J]. Mol breeding, 2015, 35(1): DOI 10.1007/s11032-015-0227-3.
[25] 肖钢,张宏军,彭琪,等.甘蓝型油菜油酸脱氢酶基因(fad2)多个拷贝的发现及分析[J].作物学报,2008,34(9):1563-1568.
[26] Chen X, Martin T, Crystal L S, et al. Three homologous genes encoding sn-glycerol-3-phosphate acyltransferase 4 exhibit different expression patterns and functional divergence in Brassica napus[J]. Plant Physiol, 2011, 155(2):851-865.
[27] Gangappa S N, Botto J F. The BBX family of plant transcription factors[J]. Trends in Plant Sci, 2014, 19(7):460-470.

油菜B-box 型锌指蛋白基因BnSTO 的克隆和表达

Cloning and Expression of BnSTO Encoding B-box-type Zinc-finger Proteins in Rapeseed

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