Abstract: Abstract: This article is the first to study on pharmacokinetic and eliminate of OTC in turbot after oral administration. The residue of OTC in turbot which was exposured to 80 mg/kg b.w. OTC solution was monitored continually by HPLC. Pharmacokinetic studies showed that the concentration-time course of the drug conformed to the characteritics of the two compartment open model with the first order absorption and a lagtime for the blood and the muscle, and the one compartment open model with first order absorption and a lagtime for the liver. OTC was slowly eliminated in turbot. The concerntration of OTC in the muscle was below LOD after 28d , and the concerntration of OTC in the liver was below LOD after 10d.