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中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 319-323.

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

梨属植物等位酶遗传多样性研究

胡红菊,王友平,张靖国,田瑞,陈启亮,杨晓平   

  • 收稿日期:2008-08-12 修回日期:2008-09-15 出版日期:2008-11-05 发布日期:2008-11-05

Allozyme Analysis of Genetic Diversity in Pyrus spp.

Hu Hongju, Wang Youping, Zhang Jingguo, Tian Rui, Chen Qiliang, Yang Xiaoping   

  • Received:2008-08-12 Revised:2008-09-15 Online:2008-11-05 Published:2008-11-05

摘要: 利用超薄平板微型聚丙烯酰胺凝胶的等电聚焦电泳技术,对286份梨材料进行了等位酶遗传变异分析。在8个酶系中共检测到19个清晰位点和82个等位基因,19个位点均为多态位点,位点最大等位基因数为6,体现出梨丰富的遗传种质多样性;不同的居群具有特有等位基因;通过82个等位基因可以将286份材料完全区分开,表明等位酶基因型指纹可用作梨品种区分与鉴定的依据。

关键词: 广占63S, 广占63S, 籼粳型父本, 产量性状, 相关分析, 通径系数

Abstract: Allozymic variation of 286 pear materials were investigated using vertical slab polyacrylamide gel electrophoresis. 82 alleles of 19 loci in 8 enzyme systems were detected. All loci are polymorphic. The maxium number of alleles in a locus is 6. The result demonstrated a rich genetic diversity in pear germplasm. some specific alleles were detected in different population. Based on multi-locos genotype fingerprinting cultivars with82 alleles, 286 pear materials were clear identified, suggesting allozyme is a useful marker for peach cultivars identification.

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