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中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 251-254.

所属专题: 生物技术 小麦

• 农学 农业基础科学 • 上一篇    下一篇

转反义trxs基因小麦种子萌发过程中碳代谢的变化

任江萍,李磊,王新国,王振云,尹钧   

  • 收稿日期:2008-09-04 修回日期:2008-09-18 出版日期:2008-11-05 发布日期:2008-11-05

Change of carbon metabolism in Transgenic Anti-sense Thioredoxin s Gene Wheat in the process of germination

Ren Jiangping, Li Lei, Wang Xinguo, Wang Zhenyun, Yin Jun   

  • Received:2008-09-04 Revised:2008-09-18 Online:2008-11-05 Published:2008-11-05

摘要: 为了揭示反义硫氧还蛋白基因(anti-trxs)在抗穗发芽小麦中的作用机制。以转反义 trxs 基因小麦株系为材料,对转基因株系和对照种子萌发过程中硫氧还蛋白h活性、淀粉酶活性、可溶性糖和淀粉含量进行了检测。结果表明,转基因小麦籽粒 trxh、α-淀粉酶和β-淀粉酶活性均显著低于对照;淀粉降解和可溶性糖生成速率明显下降。发芽1~5 d,转基因小麦种子trxh、α-淀粉酶和β-淀粉酶活性分别比对照下降24.5%、40.4%和23.0%;淀粉降解和可溶性糖生成速率分别比对照降低23.7%和23%。

关键词: 农村贫困地区, 农村贫困地区, 良性耦合, 补偿机制

Abstract: To clarify the function mechanism of antisense - thioredoxin s (anti-trxs) gene in transgenic wheat with pre-harvest resistant sprouting characteristic, the thioredoxin h and amylase activities, starch and soluble sugar contents in transgenic line and non-transgenic cultivar ‘Yumai 34’ were detected during seed germination stage. The results indicated that the activities of thioredoxin h, α- amylase and β-amylase were lowered significantly and the velocities of starch degradation and soluble sugar production decreased distinctly. During germinating days 1-5d, the activities of thioredoxin h, α- amylase and β-amylase were 24.5%, 40.4% and 23.1% lower than non-transgenic seeds, respectively. The velocities of starch degradation and soluble sugar production decreased by 23.7% and 23%, respectively.

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