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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (2): 100-104.

所属专题： 生物技术

• 农学 农业基础科学 • 上一篇    下一篇

冬枣花药愈伤组织的诱导及原生质体分离

刘晓光,刘孟军,宁强,彭艳芳,苗利军,秦子禹   

  • 收稿日期:2008-10-29 修回日期:1900-01-01 出版日期:2009-01-20 发布日期:2009-01-20

Studies on Callus Induction and Protoplast Isolation on Ziziphus jujuba Mill. cv. Dongzao

  • Received:2008-10-29 Revised:1900-01-01 Online:2009-01-20 Published:2009-01-20

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摘要： 【研究目的】研究冬枣花药愈伤组织的诱导，悬浮系的建立和培养及愈伤悬浮系原生质体的分离。【方法】以冬枣花药为试材，通过选择愈伤诱导培养基和原生质体分离所用酶的浓度找到最佳诱导和分离条件。【结论】使用1/2MS基本培养基附加TDZ0.2mg/L、NAA0.5 mg/L和 PVP2.0g/L，对诱导冬枣花药愈伤组织有较好效果；愈伤组织增殖采用培养基1/2MS+TDZ0.4 mg/L +NAA0.2 mg/L；悬浮细胞系培养采用1/2MS+TDZ0.4 mg/L +NAA0.2mg/L液体培养基；冬枣花药愈伤组织悬浮系原生质体分离时以0.6M甘露醇+0.1%MES+20~25 g/L纤维素酶，酶解时间为16h时得到的原生质体产量和活力较高。

关键词: 喜马拉雅狼蛛, 喜马拉雅狼蛛, 生物学, 行为学, 生态学, 青稞地

Abstract: 【OBJECTIVE】:the paper was studied the callus induction, the foundation and culture of the suspension, and isolatation of the protoplast of anther of dongzao. 【METHODS】 the anther of dongzao was used as material to find the best culture medium of foundation and cultivation of the suspension and the protoplast isolation conditions. 【RESULTS】it is good to indicate callus when the culture medium which was added TDZ0.2mg/L、NAA0.5 mg/L and PVP2.0g/L was used. The better culture medium of callus multiplication was 1/2MS+TDZ0.4 mg/L +NAA0.2 mg/L. The liquid culture medium that was 1/2MS+TDZ0.4 mg/L +NAA0.2 mg/L was used to the suspension. When the the suspension was used to isolation, the optimal condition was 0.6M Mannital +0.1%MES+20~25 g/L Cellulase “onozuka” R-10，the isolation time was 16h.