16份黄秋葵种质的ISSR分析

摘要/Abstract

摘要： 对Owayadei（Abelmoschus esculentus L.）、Ladies finger（Abelmoschus esculentus L.）、golder kost（Abelmoschus esculentus L）、Esoyarido（Abelmoschus esculentus L.）等16份国外黄秋葵栽培种质进行ISSR分析，结果表明，选用14条引物扩增出162个DNA片段，平均每个引物可扩增出11.6条片段，其中多态性片段102个，占扩增总片段的62.96%。利用扩增结果进行遗传距离分析，构建了分子树状图，可以把16份材料划分为4个类群。

关键词: 白花曼陀罗, 白花曼陀罗, 总生物碱, 杀线活性, 作用机理

Abstract: Sixteen Citrus resources, including Owayadei（Abelmoschus esculentus L.）, Ladies finger（Abelmoschus esculentus L.）, golder kost（Abelmoschus esculentus L.）, Esoyarido（Abelmoschus esculentus L.）have been analysed by using inter-simple sequence repeat(ISSR), and the results show that 162 pattern bands results from 16 materials ISSR-PCR by amplifying 14 primers. Among which 102(62.96%) were polymorphic. The average number of DNA bands amplified by each primer was 11.6. Based on the results of amplifying, the genetic distance was analyzed, a tree diagram was constructed by using UPGMA algorithm. The 16 Okra germes were classified into 4 group.

黄捷, ,陈晓斌,叶花兰,刘国道,. 16份黄秋葵种质的ISSR分析[J]. 中国农学通报, 2008, 24(5): 0-.

Huang Jie, , Chen Xiaobin, Ye Hualan, Liu Guodao,. The ISSR Analysis of 16 Okra Resources[J]. Chinese Agricultural Science Bulletin, 2008, 24(5): 0-.

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