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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 116-119.

• 林学 园艺 园林 • 上一篇    下一篇

广西古蓬,水浪种源马尾松天然林的ISSR分析

李志辉,陈 艺,张冬林,杨模华,蒋 燚,丁贵杰,谭晓风   

  • 收稿日期:2009-02-24 修回日期:2009-04-17 出版日期:2009-08-20 发布日期:2009-08-20

Genetic Diversity of Pinnus massoniana Populations in Gupeng, ShuilangGuangxi

Li Zhihui, Chen Yi, Zhang Donglin, Yang Mohua ,Jiang Yi, Ding Guijie, Tan Xiaofeng   

  • Received:2009-02-24 Revised:2009-04-17 Online:2009-08-20 Published:2009-08-20

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摘要： 摘　要：　利用ISSR分子标记技术对广西古蓬和水浪两个种源区的马尾松天然林中共85个样品进行遗传多样性分析，从100个ISSR引物中共筛选出10个多态性引物，对这85个马尾松个体进行PCR-ISSR扩增,其中古蓬47个样品三个组共检测到192个位点，其中多态位点数为177个，水浪38个样品两个组共检测到168个多态位点。对两个种源马尾松采用POPgene 32 软件进行分析，结果表明：在古蓬马尾松天然林群体内,总的多态位点百分率（P）为94.35%，总的Shannon信息指数（I）为0.4278，Nei’s基因多样度指数（h）为0.2765；而水浪种源的马尾松群体内，总的多态位点百分率（P）为94.38 %，总的Shannon信息指数（I）为0.5190，Nei’s基因多样度指数（h）为0.3510；表明种水平的遗传多样性较高。古蓬马尾松组间的基因分化系数（Gst）为0.0481，水浪的为0.0363。表明马尾松的主要变异存在于组间，古蓬3个组的基因流为9.8877。水浪两个组的基因流为13.2643，表明基因流不是引起马尾松组间分化的主要因素。

关键词: 高油大豆, 高油大豆, 播期, 密度, 籽粒产量, 品质

Abstract: Abstract: Genetic diversity of Pinus massoniana Lamb. （mass pine） populations were evaluated using inter simple sequence repeat (ISSR) molecular technique. Amplification was conducted with 10 primers for 85 individuals selected from 2 populations. A total of 177 polymorphic were detected in Gupeng，The percentage of polymorphic marker (P) was 94.359%.The Shannon’s information index (I) and Nei’s gene diversity (h) were 0.4728 and 0.2765, respectively, A total of 168 polymorphic were detected in Shuilang.The percentage of polymorphic marker (P) was 94.38%.The Shannon’s information index (I) and Nei’s gene diversity (h) were 0.5190 and 0.3510,which indicated a high genetic diversity at species level. Gene differentiation coefficient among Gupeng groups was 0.0481 and 0.0363 among Shuilang,1gene flow of Gupeng groups was 9.8877, gene flow of Shuilang was 13.2643,which concluded that gene flow was not the factor for genetic differentiation among groups.