关键词: 玉米, 玉米, 双列杂交, 配合力, 遗传参数

Abstract: Abstract: (Object) This experiment was conducted to investigate effect of polysaccharide from portulaca oleracea on neuronal cytoskeleton microtubule protein (tau) phosphorylation. (Method) Model for neuronal cytoskeleton was established by construction of gene expression vector and transfection vehicle of pEGFP-tau-s3 in 3T3 cell. Co-transfection of pEGFP-tau-s3 and pcDNA-GSK-3β in 3T3 cell, fluorescence microscope observation, and western blotting were used in this study.(Result) Result showed that polysaccharide from Portulaca oleracea restrained effects of GSK kinase on tau phosphorylation. (Inclusion) These data suggest that Portulaca oleracea might have protection effect on neuronal cytoskeleton microtubule.