关键词: 白僵蚕, 白僵蚕, 高效毛细管电泳, 指纹图谱

Abstract:

SRAP (sequence-related amplified polymorphisms) is based on random amplification from sequences in coding sequences of ORFs which generally contain many GC bases and in promoter and intron sequences with an AATT core in 3’ region. The exon-intron markers often results with relatively high DNA polymorphism because the interval sequence length between intron and extron varies in genomes of different individuals. The SRAP marker system is a relatively recently developed DNA polymorphism technique with several advantages over other systems: simplicity, reasonable throughput rate, low cost, high reliability, easy isolation of bands for sequencing, which received concerns widely. In the last few years, it has been used in genetic diversity analysis in many plants like Brassica vegetables, potato, garlic, celery, rice, cherry, and apple. In this paper, the technical mechanism of SRAP, process of experiment protocol and its application in genetic diversity analysis of vegetable plants had been widely reviewed.