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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 22-24.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

高效降解角蛋白菌株的分离筛选与鉴定

王政 郭士兵 姚大伟 陶艳华 杨德吉   

  • 收稿日期:2009-04-11 修回日期:2009-04-27 出版日期:2009-09-20 发布日期:2009-09-20

Isolation and Identification of Keratin-Degrading Bacillus Thuringiensis NJY1

  • Received:2009-04-11 Revised:2009-04-27 Online:2009-09-20 Published:2009-09-20

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摘要：

为从环境中分离高效降解羽毛角蛋白的细菌，采用以羽毛粉为唯一碳源和氮源的培养基筛选，结果得到一株高效降解羽毛角蛋白的NJY1（CGMCC No.2337）菌株。通过对该菌株形态特征观察，生理生化特性测定和16S rRNA分析（Genbank登陆号：EU624205），该菌株与苏云金芽孢杆菌(Bacillus thuringiensis)的相似性达100％，初步认定该菌株为苏云金芽孢杆菌。采用该菌株经过72h发酵，角蛋白降解率达72.5％；2%羽毛发酵液中可溶性蛋白含量达到25.2mg/mL。本试验为微生物发酵降解羽毛粉，生产高质量的蛋白饲料提供了优良菌株。

关键词: 光皮木瓜, 光皮木瓜, 顶空固相微萃取, GC-MS, 芳香物质

Abstract:

To obtain high efficient keratin-degraded bacteria, strain NJY1 from environment soil was isolated by feather meal as its sole source of carbon, nitrogen. Strain NJY1 was identified as Bacillus thuringiensis based on morphology observation, physicochemical characteristics and 16S rRNA sequence analysis (GenBank ID: EU624205) due to100% homology with it. The degradation rate of feather was up to 72.5% after 72 h of fermentation at 37 °C, and soluble protein concentration in fermented liquid was 25.2mg/mL. We provided a high efficient keratin-degraded bacterium to product high quality protein feed.