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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (18): 18-21.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪LXRα基因的克隆、序列分析及表达研究

于浩   

  • 收稿日期:2009-04-27 修回日期:2009-06-07 出版日期:2009-09-20 发布日期:2009-09-20

Cloning, Sequence and Express Patterns Analysis of Porcine LXRα Gene

  • Received:2009-04-27 Revised:2009-06-07 Online:2009-09-20 Published:2009-09-20

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639

摘要：

采用 RT-PCR 结合克隆测序的方法,从猪的肝脏组织中克隆出了猪基因的 Liver X Receptors α cDNA 序列，编码区长度1344bp，编码447个氨基酸，基于Pfam数据库发现存在一个锌指蛋白和核受体的配体结合区；系统发育分析发现猪的LXRα所在的哺乳动物类群非常保守，与鸡和斑马鱼所构成的类群存在较大遗传距离；在大白猪和杂种野猪的脂肪组织中检测表明LXRα基因的表达随着月龄的增加也不断增加，并日趋稳定，而杂种野猪在9月龄开始高表达，12月龄最高。

关键词: 番茄, 番茄, 营养品质, 遗传多样性

Abstract:

Cloning and sequencing the Liver X Receptors α cDNA from porcine liver tissues by RT-PCR, the coding region length was 1344bp, coding 447 Amino Acids, Zinc finger and ligand-binding domain of nuclear hormone receptor are found based on the Pfam database, phylogenetic result showed that the LXRα genes in Mammalia clade were very conserved, the genetic distance between the Mammalia clade and non-Mammalia clade including chicken and zebrafish, LXRα mRNA level were detected in the adipose tissues showed that LXRα mRNA expression increased from 3-month to 12-month, the mRNA level was the most high in 9-month in large white pig, but the LXRα mRNA expression were detected in 9 month, and the expression in 12-month was high in crossed-bred wild pig.