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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (19): 182-185.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

基于DNA分子标记技术研究的10个特色茶花品种遗传差异分析

王晓锋,陈根金,郑红玉,叶海仁,郑蔚虹   

  • 收稿日期:2009-07-06 修回日期:2009-07-28 出版日期:2009-10-05 发布日期:2009-10-05
  • 基金资助:

    温州特色茶花品种基因组水平遗传多样性研究;基于遗传背景研究的温州山茶花遗传关系研究及开发应用

Genetic diversities among 10 representative Camellia cultivars based on DNA molecular marker

  • Received:2009-07-06 Revised:2009-07-28 Online:2009-10-05 Published:2009-10-05

PDF (PC)

694

摘要：

采用随机扩增多态性DNA (RAPD)技术对10个温州特色茶花品种的遗传多样性进行研究。15条十聚体随机引物共检测到105个清晰扩增位点，其中多态性位点79个，多态位点百分率为77.54%。遗传相似性分析表明：10个样品遗传距离在0.2236～0.4777之间，其中样品大朱砂与快乐王遗传距离最大（0.4905），而金盘荔枝与快乐王最小（0.2236）；聚类结果显示：在遗传相似系数为 0.62时，10个茶花品种共分三组，其中样品弁天神乐、点雪分别自成一组，其余样品聚类一起。

关键词: 土地利用方式, 土地利用方式, 土壤有机碳, 机械组成

Abstract:

Abstract: Genetic diversities among 10 representative camellia cultivars in Wenzhou were investigated by RAPD technology（random amplification polymorphic DNA）. The results indicated that 105 bands were produced with 15 random primers, and the genetically polymorphic bands numbered 79 accounting for 77.54%. Genetic distance analysis represented genetic distances among 10 samples ranged from 0.2236 to 0.4777 with the biggest value between samples DaZhuS and Gay Chielftain and the lowest between JinPanLZ and Gay Chielftain. Dendrogram by UPGMA software disclosed that 3 groups were produced at the similarity coefficient of 0.62, with sample Benten-kagura and DianXue isolated and the rest clustered together.